Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,785	T→C	L302P (CTG→CCG)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,785	0	T	C	100.0%	207.1 / NA	57	L302P (CTG→CCG)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

AGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCA  >  NC_000913/1931680‑1931918                                                                                                          |                                                                                                                                      aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATg                                                                                                                                  >  1:355597/1‑111 (MQ=255) aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATg                                                                                                                                  <  2:355597/111‑1 (MQ=255) aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTgg                                                                                                      >  1:1264463/1‑139 (MQ=255) aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTgg                                                                                                      >  2:1588582/1‑139 (MQ=255)              tggtgATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTAc                                                                                                                               >  1:1559935/1‑101 (MQ=255)              tggtgATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTAc                                                                                                                               <  2:1559935/101‑1 (MQ=255)                    tgaGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCgg                                                                                                          >  2:1250485/1‑116 (MQ=255)                    tgaGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCgg                                                                                                          <  1:1250485/116‑1 (MQ=255)                    tgaGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGt                                                                                   >  1:181049/1‑139 (MQ=255)                          gATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   <  1:1517958/101‑1 (MQ=255)                          gATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   >  2:1517958/1‑101 (MQ=255)                           aTTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAg                                                                            >  1:278322/1‑139 (MQ=255)                               tCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc                                                                        <  2:278322/139‑1 (MQ=255)                                  gTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGc                                                                     >  1:1596910/1‑139 (MQ=255)                                          tcCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCt                                                                                                                     <  2:31261/83‑1 (MQ=255)                                          tcCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCt                                                                                                                     >  1:31261/1‑83 (MQ=255)                                          tcCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTc                                                             >  1:145270/1‑139 (MQ=255)                                             ccTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                          <  2:1596910/139‑1 (MQ=255)                                                 gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTGGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  1:1196848/1‑139 (MQ=255)                                                 gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   >  1:498212/1‑78 (MQ=255)                                                 gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   >  1:553576/1‑78 (MQ=255)                                                 gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   <  2:498212/78‑1 (MQ=255)                                                 gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   <  2:553576/78‑1 (MQ=255)                                                 gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  2:540313/1‑139 (MQ=255)                                                  tCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                          <  1:42822/134‑1 (MQ=255)                                                  tCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                          >  2:42822/1‑134 (MQ=255)                                                       aTGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGAc                                                <  1:540313/139‑1 (MQ=255)                                                       aTGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGAc                                                <  2:1264463/139‑1 (MQ=255)                                                              cGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATAGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAg                                         <  1:1183775/139‑1 (MQ=255)                                                                ggATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc                                                                        >  2:614994/1‑106 (MQ=255)                                                                ggATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc                                                                        <  1:614994/106‑1 (MQ=255)                                                                 gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGc                                                                                                                           <  1:333415/54‑1 (MQ=255)                                                                 gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGc                                                                                                                           >  2:333415/1‑54 (MQ=255)                                                                 gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAAt                                      >  2:865985/1‑139 (MQ=255)                                                                    ggTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   <  1:373919/139‑1 (MQ=255)                                                                     gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      <  2:307555/119‑1 (MQ=255)                                                                     gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  1:307555/1‑119 (MQ=255)                                                                     gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   <  2:1543077/138‑1 (MQ=255)                                                                     gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   >  1:1543077/1‑138 (MQ=255)                                                                         cGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGtt                              <  1:865985/139‑1 (MQ=255)                                                                              aTTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATa                         <  2:145270/139‑1 (MQ=255)                                                                              aTTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATa                         <  2:1196848/139‑1 (MQ=255)                                                                                          ctctcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGa                                                                                                                 <  2:913429/39‑1 (MQ=255)                                                                                          ctctcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGa                                                                                                                 >  1:913429/1‑39 (MQ=255)                                                                                             tcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   <  1:676452/114‑1 (MQ=255)                                                                                             tcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   >  2:676452/1‑114 (MQ=255)                                                                                                   tttGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGt                                    <  1:583545/107‑1 (MQ=255)                                                                                                   tttGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGt                                    >  2:583545/1‑107 (MQ=255)                                                                                                     tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTAt                                                               >  2:422066/1‑78 (MQ=255)                                                                                                     tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTAt                                                               <  1:422066/78‑1 (MQ=255)                                                                                                     tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGt                                            <  2:187432/97‑1 (MQ=255)                                                                                                     tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGt                                            >  1:187432/1‑97 (MQ=255)                                                                                                     tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCa                  >  2:1463650/1‑123 (MQ=255)                                                                                                     tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCa                  <  1:1463650/123‑1 (MQ=255)                                                                                                     tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGACGTAGGCGCa  >  2:254330/1‑139 (MQ=255)                                                                                                      gCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGc                                                                              <  2:16137/62‑1 (MQ=255)                                                                                                      gCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGc                                                                              >  1:16137/1‑62 (MQ=255)                                                                                                       ccccGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  2:1463525/1‑85 (MQ=255)                                                                                                       ccccGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      <  1:1463525/85‑1 (MQ=255)                                                                                                          |                                                                                                                                      AGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCA  >  NC_000913/1931680‑1931918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑