Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,090,046	G→A	*17* (TAG→TAA)	hisL →	his operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,090,046	0	G	A	100.0%	249.8 / NA	76	*17* (TAG→TAA)	hisL	his operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/40);  total (36/40)

ATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089916‑2090180                                                                                                                                   |                                                                                                                                       ataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAg                                                                                                                                <  1:702585/139‑1 (MQ=255)      aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  1:326062/139‑1 (MQ=255)        ggCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                         >  2:825659/2‑139 (MQ=255)        aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                         >  2:12918/1‑139 (MQ=255)        aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           >  1:1033226/1‑137 (MQ=255)        aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  2:1033226/137‑1 (MQ=255)         gCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg                                                                                                                        <  2:837487/139‑1 (MQ=255)          cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                       >  2:731601/1‑139 (MQ=255)          cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                       >  2:946403/1‑139 (MQ=255)                 tttCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  1:731601/139‑1 (MQ=255)                     tAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          >  1:1004368/1‑125 (MQ=255)                     tAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          <  2:1004368/125‑1 (MQ=255)                        cGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                   <  2:640348/113‑1 (MQ=255)                        cGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                   >  1:640348/1‑113 (MQ=255)                         gTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         <  1:141745/138‑1 (MQ=255)                         gTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         >  2:141745/1‑138 (MQ=255)                         gTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                        <  1:946403/139‑1 (MQ=255)                           gAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  2:1493892/139‑1 (MQ=255)                           gAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  1:693502/139‑1 (MQ=255)                            aaaGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  1:50648/117‑1 (MQ=255)                            aaaGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           >  2:50648/1‑117 (MQ=255)                                   tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCg                                                                                                                             >  2:1393169/1‑108 (MQ=255)                                   tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCg                                                                                                                             <  1:1393169/108‑1 (MQ=255)                                   tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  2:585520/139‑1 (MQ=255)                                   tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  1:12918/139‑1 (MQ=255)                                   tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  1:725760/139‑1 (MQ=255)                                     tAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAg                                                                                                                                >  2:1400885/1‑103 (MQ=255)                                     tAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAg                                                                                                                                <  1:1400885/103‑1 (MQ=255)                                           aaaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCAt                                                                                      <  1:1493208/139‑1 (MQ=255)                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               <  1:517712/97‑1 (MQ=255)                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               >  2:517712/1‑97 (MQ=255)                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  1:1021254/1‑112 (MQ=255)                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  2:1021254/112‑1 (MQ=255)                                            aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                     >  1:1025867/1‑139 (MQ=255)                                             aaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      >  2:379674/1‑121 (MQ=255)                                             aaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  1:379674/121‑1 (MQ=255)                                                       ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                     <  1:1307071/128‑1 (MQ=255)                                                       ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                     >  2:1307071/1‑128 (MQ=255)                                                          aaTAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAg                                                                       >  2:950063/1‑139 (MQ=255)                                                                cATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               <  1:1069106/77‑1 (MQ=255)                                                                cATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               >  2:1069106/1‑77 (MQ=255)                                                                 aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           >  1:206724/1‑80 (MQ=255)                                                                 aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  2:206724/80‑1 (MQ=255)                                                                         agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                     <  2:978769/110‑1 (MQ=255)                                                                         agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATg                                                                                     >  1:978769/1‑110 (MQ=255)                                                                           aCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           >  1:1588061/1‑70 (MQ=255)                                                                           aCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  2:1588061/70‑1 (MQ=255)                                                                                     cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           <  1:1314145/76‑1 (MQ=255)                                                                                     cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           >  2:1314145/1‑76 (MQ=255)                                                                                       cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         >  1:380224/1‑76 (MQ=255)                                                                                       cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         <  2:380224/76‑1 (MQ=255)                                                                                       cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   >  2:565629/1‑98 (MQ=255)                                                                                       cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   <  1:565629/98‑1 (MQ=255)                                                                                                tttAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  1:950063/139‑1 (MQ=255)                                                                                                         caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               >  1:73064/1‑52 (MQ=255)                                                                                                         caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               <  2:73064/52‑1 (MQ=255)                                                                                                         caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTc                                                                                                 <  2:120790/66‑1 (MQ=255)                                                                                                         caccatcatcaccatcatCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTc                                                                                                 >  1:120790/1‑66 (MQ=255)                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGAtgtgtg                                                                                                                      <  1:629967/44‑1 (MQ=255)                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGAtgtgtg                                                                                                                      >  2:629967/1‑44 (MQ=255)                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  1:1098063/129‑1 (MQ=255)                                                                                                          accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 >  2:1098063/1‑129 (MQ=255)                                                                                                              tcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGt                   >  2:1141071/1‑139 (MQ=255)                                                                                                                       tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACa                                                                                                            >  2:663891/1‑41 (MQ=255)                                                                                                                       tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACa                                                                                                            <  1:663891/41‑1 (MQ=255)                                                                                                                        catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  2:789455/46‑1 (MQ=255)                                                                                                                        catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      >  1:789455/1‑46 (MQ=255)                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           >  2:56723/1‑39 (MQ=255)                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           <  1:56723/39‑1 (MQ=255)                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa              >  2:583503/1‑132 (MQ=255)                                                                                                                          tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa              <  1:583503/132‑1 (MQ=255)                                                                                                                           ccTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                >  1:469969/1‑97 (MQ=255)                                                                                                                           ccTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                <  2:469969/97‑1 (MQ=255)                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt        <  1:247107/133‑1 (MQ=255)                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt        >  2:247107/1‑133 (MQ=255)                                                                                                                               aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga  >  1:1582219/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       ATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089916‑2090180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑