Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,104,494 C→T V6I (GTC→ATC) 
*197* (TAG→TAA) 		 wbbK ←
wbbJ ←		 lipopolysaccharide biosynthesis protein
putative lipopolysaccharide biosynthesis O‑acetyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,104,4940CT100.0% 233.9 / NA 73V6I (GTC→ATC) 
*197* (TAG→TAA) 		wbbK
wbbJ		lipopolysaccharide biosynthesis protein
putative lipopolysaccharide biosynthesis O‑acetyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/39);  total (34/39)

TTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCAT  >  NC_000913/2104363‑2104621
                                                                                                                                   |                                                                                                                               
ttctttTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCtt                                                                                                                          <  2:671609/139‑1 (MQ=255)
  ctttTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCtttt                                                                                                                        >  2:35580/1‑139 (MQ=255)
      tAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                                    <  1:673507/139‑1 (MQ=255)
                    aaTGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCa                                                                                                      >  2:152884/1‑139 (MQ=255)
                      tGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTc                                                                                                                      <  2:1393404/121‑1 (MQ=255)
                      tGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTc                                                                                                                      >  1:1393404/1‑121 (MQ=255)
                              tGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTAtt                                                                                            >  2:416960/1‑139 (MQ=255)
                                cGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                   <  2:279675/130‑1 (MQ=255)
                                cGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                   >  1:279675/1‑130 (MQ=255)
                                  aTAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                                    <  2:145013/111‑1 (MQ=255)
                                  aTAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                                    >  1:145013/1‑111 (MQ=255)
                                   tAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAttt                                                                                                             >  1:504037/1‑117 (MQ=255)
                                   tAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAttt                                                                                                             <  2:504037/117‑1 (MQ=255)
                                   tAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                               <  2:342882/115‑1 (MQ=255)
                                   tAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                               >  1:342882/1‑115 (MQ=255)
                                                   tttATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                       <  1:416960/139‑1 (MQ=255)
                                                     tattattaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                     <  1:35580/139‑1 (MQ=255)
                                                          ttaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTcc                                                                                                                     >  1:885795/1‑86 (MQ=255)
                                                          ttaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTcc                                                                                                                     <  2:885795/86‑1 (MQ=255)
                                                          ttaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGc                                                                <  1:169570/139‑1 (MQ=255)
                                                          ttaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGc                                                                <  1:778174/139‑1 (MQ=255)
                                                            aGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCaa                                                              <  1:152884/139‑1 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAAGGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                   <  2:1126198/97‑1 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                               <  1:49125/85‑1 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                               <  2:1422936/85‑1 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                               >  2:49125/1‑85 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAt                                                                                                               >  1:1422936/1‑85 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                   >  1:1126198/1‑97 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGc                                                                            <  2:572196/120‑1 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGc                                                                            >  1:572196/1‑120 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                     >  2:293472/1‑127 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                     <  1:293472/127‑1 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGc                                                                >  1:126503/1‑132 (MQ=255)
                                                                 ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGc                                                                <  2:126503/132‑1 (MQ=255)
                                                                  tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAtt                                                                                                              >  2:362910/1‑85 (MQ=255)
                                                                  tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAtt                                                                                                              <  1:362910/85‑1 (MQ=255)
                                                                  tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                          >  2:898930/1‑89 (MQ=255)
                                                                  tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                          <  1:898930/89‑1 (MQ=255)
                                                                  tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAg                                                        >  2:65564/1‑139 (MQ=255)
                                                                  tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAg                                                        >  2:1123506/1‑139 (MQ=255)
                                                                   gcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATTCTTTTTccc                                                                                                                    <  1:595079/78‑1 (MQ=255)
                                                                   gcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                                    >  2:595079/1‑78 (MQ=255)
                                                                   gcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGt                                                       >  1:500030/1‑139 (MQ=255)
                                                                    ccAAAAATTTTTTCATAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                         >  1:1118505/1‑136 (MQ=255)
                                                                    ccAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTCGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTCTTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                         <  2:1118505/136‑1 (MQ=255)
                                                                        aaaTTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATg                                                                                                                             <  2:1300196/64‑1 (MQ=255)
                                                                        aaaTTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATg                                                                                                                             >  1:1300196/1‑64 (MQ=255)
                                                                                tCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATa                                          <  1:1123506/139‑1 (MQ=255)
                                                                                 cAAAATGGTAAATGGACCGCCAGTGGTTAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTTTGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAg                                         <  1:963457/139‑1 (MQ=255)
                                                                                   aaaTGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGaa                                       <  1:65564/139‑1 (MQ=255)
                                                                                        gTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACctct                                  <  2:500030/139‑1 (MQ=255)
                                                                                                aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                                     <  1:1130218/64‑1 (MQ=255)
                                                                                                aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                                     >  2:1130218/1‑64 (MQ=255)
                                                                                                aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                       >  2:836120/1‑94 (MQ=255)
                                                                                                aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                       <  1:836120/94‑1 (MQ=255)
                                                                                                aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAg                          >  2:759408/1‑139 (MQ=255)
                                                                                                  cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAtt                                                                                                              >  2:1519468/1‑53 (MQ=255)
                                                                                                  cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAtt                                                                                                              <  1:1519468/53‑1 (MQ=255)
                                                                                                  cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                         >  1:567662/1‑90 (MQ=255)
                                                                                                  cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTgg                                                                         <  2:567662/90‑1 (MQ=255)
                                                                                                  cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                       <  2:1218494/92‑1 (MQ=255)
                                                                                                  cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                       >  1:1218494/1‑92 (MQ=255)
                                                                                                  cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGaa                        >  1:1402186/1‑139 (MQ=255)
                                                                                                  cGCCAGTGATAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGaa                        >  2:204693/1‑139 (MQ=255)
                                                                                                                ttGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTAc          <  2:1402186/139‑1 (MQ=255)
                                                                                                                 tGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACg         <  2:530679/139‑1 (MQ=255)
                                                                                                                  gACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGa        <  1:759408/139‑1 (MQ=255)
                                                                                                                   aCCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                     >  2:503081/1‑77 (MQ=255)
                                                                                                                   aCCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACt                                                                     <  1:503081/77‑1 (MQ=255)
                                                                                                                   aCCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGc                                                                <  2:934085/82‑1 (MQ=255)
                                                                                                                   aCCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGc                                                                >  1:934085/1‑82 (MQ=255)
                                                                                                                    ccGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACt      >  2:142939/1‑139 (MQ=255)
                                                                                                                        aGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCAt  <  1:204693/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                               
TTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCAT  >  NC_000913/2104363‑2104621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑