Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,139,759	C→T	*618* (TAG→TAA)	asmA ←	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,139,759	0	C	T	100.0%	211.9 / NA	66	*618* (TAG→TAA)	asmA	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/33);  total (33/33)

AGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGA  >  NC_000913/2139634‑2139876                                                                                                                              |                                                                                                                      aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                          <  2:398003/139‑1 (MQ=255) aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                          >  2:1424008/1‑139 (MQ=255) aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                          >  2:737534/1‑139 (MQ=255) aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                          >  1:398003/1‑139 (MQ=255)   gAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGc                                                                                                        <  2:638925/139‑1 (MQ=255)                 cGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATc                                                                                          >  1:1170215/1‑139 (MQ=255)                    aaCAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                       <  1:300691/139‑1 (MQ=255)                         ccTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                     <  1:814506/120‑1 (MQ=255)                         ccTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                     >  2:814506/1‑120 (MQ=255)                               aTCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            <  2:1061669/139‑1 (MQ=255)                               aTCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            <  1:737534/139‑1 (MQ=255)                                  ttACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTCCTACACATCTTTGCCATTACGGGAAt                                                                         <  1:829770/139‑1 (MQ=255)                                   tACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATc                                                                        >  2:402679/1‑139 (MQ=255)                                            aGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                          <  2:286560/96‑1 (MQ=255)                                            aGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                          >  1:286560/1‑96 (MQ=255)                                               atatAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTc                                                            >  2:1124271/1‑139 (MQ=255)                                                        tgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACg                                                                              <  2:1079417/112‑1 (MQ=255)                                                        tgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACg                                                                              >  1:1079417/1‑112 (MQ=255)                                                        tgCCGCCACGAACGCCAGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            <  2:1668749/114‑1 (MQ=255)                                                        tgCCGCCACGAACGCCAGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            >  1:1668749/1‑114 (MQ=255)                                                          ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAActtctt                                                                                                <  2:1473867/92‑1 (MQ=255)                                                          ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAActtctt                                                                                                >  1:1473867/1‑92 (MQ=255)                                                          ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                       >  2:207969/1‑101 (MQ=255)                                                          ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                       <  1:207969/101‑1 (MQ=255)                                                          ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAt                                                 >  1:351102/1‑139 (MQ=255)                                                             ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTcaca                                                                                            <  2:1644089/93‑1 (MQ=255)                                                             ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTcaca                                                                                            >  1:1644089/1‑93 (MQ=255)                                                             ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            <  2:916147/109‑1 (MQ=255)                                                             ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            <  2:1554997/109‑1 (MQ=255)                                                             ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            >  1:1554997/1‑109 (MQ=255)                                                             ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            >  1:916147/1‑109 (MQ=255)                                                             ccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCa                                              >  2:1471221/1‑139 (MQ=255)                                                              cACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                <  2:516043/104‑1 (MQ=255)                                                              cACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                >  1:516043/1‑104 (MQ=255)                                                                  aaCGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCGTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGa                                         >  2:1043005/1‑139 (MQ=255)                                                                      ccTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGtt                                     <  1:1471221/139‑1 (MQ=255)                                                                       cTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGa                                                                           <  1:156361/100‑1 (MQ=255)                                                                       cTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGa                                                                           >  2:156361/1‑100 (MQ=255)                                                                          aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGc                                 >  2:802562/1‑139 (MQ=255)                                                                          aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTATTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGc                                 >  2:219784/1‑139 (MQ=255)                                                                           tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                <  1:1124271/139‑1 (MQ=255)                                                                           tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                <  2:351102/139‑1 (MQ=255)                                                                               aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTATTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGt                            <  1:219784/139‑1 (MQ=255)                                                                                cGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           <  2:1170215/139‑1 (MQ=255)                                                                                    ttACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGt                       >  2:839317/1‑139 (MQ=255)                                                                                     tACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTGCGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           >  2:212643/1‑134 (MQ=255)                                                                                     tACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTGCGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           <  1:212643/134‑1 (MQ=255)                                                                                             ttATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACttcttc                                                                                               >  1:1361319/1‑58 (MQ=255)                                                                                             ttATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACttcttc                                                                                               <  2:1361319/58‑1 (MQ=255)                                                                                               aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACCTCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGTCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg            <  1:839317/139‑1 (MQ=255)                                                                                               aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                <  2:1560037/119‑1 (MQ=255)                                                                                               aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                >  1:1560037/1‑119 (MQ=255)                                                                                                 cAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgct          <  1:1043005/139‑2 (MQ=255)                                                                                                    gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           <  2:634368/119‑1 (MQ=255)                                                                                                    gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           >  2:1072947/1‑119 (MQ=255)                                                                                                    gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           >  1:634368/1‑119 (MQ=255)                                                                                                    gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                           <  1:1072947/119‑1 (MQ=255)                                                                                                    gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTa       >  2:913918/1‑139 (MQ=255)                                                                                                     ccTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGc                                                                                     <  2:393819/60‑1 (MQ=255)                                                                                                     ccTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGc                                                                                     >  1:393819/1‑60 (MQ=255)                                                                                                        aCAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTg              >  2:207934/1‑128 (MQ=255)                                                                                                        aCAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTg              <  1:207934/128‑1 (MQ=255)                                                                                                         cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa  <  2:1301247/139‑1 (MQ=255)                                                                                                         cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa  >  1:1301247/1‑139 (MQ=255)                                                                                                                      cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            <  2:596832/52‑1 (MQ=255)                                                                                                                      cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                            >  1:596832/1‑52 (MQ=255)                                                                                                                              |                                                                                                                      AGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGA  >  NC_000913/2139634‑2139876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑