Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,171,398	G→A	*289* (TAG→TAA)	insF1 →	IS3 transposase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,171,398	0	G	A	100.0%	98.9 / NA	32	*289* (TAG→TAA)	insF1	IS3 transposase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/13);  total (19/13)

TTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTT  >  NC_000913/2171303‑2171533                                                                                                |                                                                                                                                        tttAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGt                                                                                              >  2:1585247/1‑139 (MQ=255) tttAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGt                                                                                              >  1:658751/1‑139 (MQ=255)  ttAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTc                                                                                                <  1:115679/136‑1 (MQ=38)  ttAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTc                                                                                                >  2:115679/1‑136 (MQ=255)           tCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCAtttt                                                                                    >  2:491165/1‑139 (MQ=255)           tCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCATGGATCAtttt                                                                                    >  2:1084036/1‑139 (MQ=255)              aaTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCAtt                                                                                      >  2:774442/1‑134 (MQ=255)              aaTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCAtt                                                                                      <  1:774442/134‑1 (MQ=255)              aaTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGAc                                                                                 >  2:728298/1‑139 (MQ=255)                    aTTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTgccgc                                                                           >  2:1429615/1‑139 (MQ=255)                         aaTCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTgcg                                                                      <  1:1084036/139‑1 (MQ=255)                             ggTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCAt                                                                                                      <  2:583175/103‑1 (MQ=18)                             ggTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCAt                                                                                                      >  1:583175/1‑103 (MQ=18)                              gTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTAtt                                                                 >  1:746023/1‑139 (MQ=255)                                  cggcggcACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCt                                                                    >  1:1460582/1‑132 (MQ=255)                                  cggcggcACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCt                                                                    <  2:1460582/132‑1 (MQ=255)                                     cggcACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTc                                                                                                >  1:151722/1‑101 (MQ=255)                                     cggcACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTc                                                                                                <  2:151722/101‑1 (MQ=255)                                                             aGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTgcg                                                                      <  1:58907/103‑1 (MQ=255)                                                             aGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTgcg                                                                      >  2:58907/1‑103 (MQ=255)                                                                         tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGt                                                                                                         >  1:188463/1‑56 (MQ=255)                                                                         tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGt                                                                                                         <  2:188463/56‑1 (MQ=255)                                                                         tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAAcc                      >  1:665113/1‑139 (MQ=255)                                                                         tttGAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAAcc                      >  2:666409/1‑139 (MQ=255)                                                                         tttGAAAAAAAGAACCTCGCTTAAGGCTGTGTCCATATTAAGTGGGTAGGAACAGTGCTTCCTTCGTTGATCATTTTTACCGCCGCCTGCGCTATTCTGTTTTTTTGCGCGCTGGCGGGCTGTAATCGCTCTTTAAAcc                      >  1:1430094/1‑139 (MQ=255)                                                                            gAAAACAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGa                   <  2:1430094/139‑1 (MQ=255)                                                                             aaaaCAAGAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGAc                  <  1:491165/139‑1 (MQ=255)                                                                                    gAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGa                                                                                           >  2:1525369/1‑59 (MQ=255)                                                                                    gAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGa                                                                                           <  1:1525369/59‑1 (MQ=255)                                                                                    gAACCTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTgg           >  1:525101/1‑139 (MQ=255)                                                                                       ccTCGCTTAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGtc        <  2:525101/139‑1 (MQ=255)                                                                                             ttAAGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGtt  <  2:658751/139‑1 (MQ=255)                                                                                                |                                                                                                                                        TTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGTCTCGGTT  >  NC_000913/2171303‑2171533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑