Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 219,364 T→C P77P (CCA→CCG)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913219,3640TC100.0% 187.9 / NA 52P77P (CCA→CCG) tsaAtRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/28);  total (24/28)

CTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAAT  >  NC_000913/219248‑219480
                                                                                                                    |                                                                                                                    
cTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgc                                                                                                >  2:203566/1‑139 (MQ=255)
 tGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                               <  1:1364059/139‑1 (MQ=255)
  gTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgccg                                                                                              <  2:1171556/139‑1 (MQ=255)
         tGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCACGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCa                                                                                       <  2:1176292/139‑1 (MQ=255)
             aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                   >  2:1558393/1‑139 (MQ=255)
             aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                   <  1:1558393/139‑1 (MQ=255)
                 aacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACg                                                                                                                 <  2:480346/105‑1 (MQ=255)
                 aacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACg                                                                                                                 >  1:480346/1‑105 (MQ=255)
                              tctACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                  <  1:203566/139‑1 (MQ=255)
                                  ccAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                              <  2:385660/139‑1 (MQ=255)
                                       gACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATa                                                         >  1:828051/1‑139 (MQ=255)
                                        tcATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACg                                                                                                                 <  1:986714/81‑1 (MQ=255)
                                        aCATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACg                                                                                                                 >  2:986714/1‑82 (MQ=255)
                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgccgcc                                                                                            >  1:1243278/1‑99 (MQ=255)
                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgccgcc                                                                                            <  2:1243278/99‑1 (MQ=255)
                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAg                                                                 <  2:712760/126‑1 (MQ=255)
                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAg                                                                 >  1:712760/1‑126 (MQ=255)
                                                 ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                   <  1:1031532/119‑1 (MQ=255)
                                                 ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                   >  2:1031532/1‑119 (MQ=255)
                                                              gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACg                                                                                                                 <  2:1287985/60‑1 (MQ=255)
                                                              gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACg                                                                                                                 >  1:1287985/1‑60 (MQ=255)
                                                              gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGa                                                                                                       >  1:866452/1‑70 (MQ=255)
                                                              gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGa                                                                                                       <  2:866452/70‑1 (MQ=255)
                                                              gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                               >  1:13267/1‑126 (MQ=255)
                                                              gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                               <  2:13267/126‑1 (MQ=255)
                                                              gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCcg                                  >  1:847396/1‑139 (MQ=255)
                                                               aaaGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGaaa                                                                          <  2:57612/98‑1 (MQ=255)
                                                               aaaGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGaaa                                                                          >  1:57612/1‑98 (MQ=255)
                                                               aaaGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCcgc                                 >  2:168379/1‑139 (MQ=255)
                                                                        cGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAACCGCCTTCCATCGTTTGATGAAAGACGa                                                                     <  2:1626546/94‑1 (MQ=255)
                                                                        cGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAACCGCCTTCCATCGTTTGATGAAAGACGa                                                                     >  1:1626546/1‑94 (MQ=255)
                                                                         gtgtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTc                       <  1:168379/139‑1 (MQ=255)
                                                                          tgtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                   >  1:581485/1‑94 (MQ=255)
                                                                          tgtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                   <  2:581485/94‑1 (MQ=255)
                                                                            ttGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGc                    >  1:952600/1‑139 (MQ=255)
                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaa                                                                    <  1:1167485/90‑1 (MQ=255)
                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaa                                                                    >  2:1167485/1‑90 (MQ=255)
                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAg                                                                 <  1:838124/93‑1 (MQ=255)
                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAg                                                                 >  2:838124/1‑93 (MQ=255)
                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGcc                   <  2:1078728/139‑1 (MQ=255)
                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGcc                   <  2:847396/139‑1 (MQ=255)
                                                                                              tGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAAt  <  2:631200/139‑1 (MQ=255)
                                                                                                gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                 <  2:123570/42‑1 (MQ=255)
                                                                                                gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                 >  1:123570/1‑42 (MQ=255)
                                                                                                gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                   >  2:542703/1‑72 (MQ=255)
                                                                                                gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                   <  1:542703/72‑1 (MQ=255)
                                                                                                gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGcc                   <  2:1175211/120‑1 (MQ=255)
                                                                                                gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGcc                   >  1:1175211/1‑120 (MQ=255)
                                                                                                          cgAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaa                                                                    >  2:1333967/1‑61 (MQ=255)
                                                                                                          cgAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaa                                                                    <  1:1333967/61‑1 (MQ=255)
                                                                                                               cgcgGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGaa                              <  1:912379/94‑1 (MQ=255)
                                                                                                               cgcgGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGaa                              >  2:912379/1‑94 (MQ=255)
                                                                                                                    |                                                                                                                    
CTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAAT  >  NC_000913/219248‑219480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑