Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,013	T→C	R188R (CGT→CGC)	yeiP →	elongation factor P‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,013	0	T	C	100.0%	226.8 / NA	63	R188R (CGT→CGC)	yeiP	elongation factor P‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/30);  total (33/30)

GGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCC  >  NC_000913/2265886‑2266146                                                                                                                                |                                                                                                                                      gggCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACt                                                                                                                            >  2:771951/1‑139 (MQ=255)      tCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCCGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTAAGc                                                                                                                       >  2:1077703/1‑139 (MQ=255)                  cGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                           <  1:771951/139‑1 (MQ=255)                    tAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAg                                                                                                         >  1:289190/1‑139 (MQ=255)                            cGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                                 <  2:289190/139‑1 (MQ=255)                              gCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGc                                                                                                                       >  2:39558/1‑115 (MQ=255)                              gCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGc                                                                                                                       <  1:39558/115‑1 (MQ=255)                                              gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCAt                                                                                                                  >  2:184284/1‑104 (MQ=255)                                              gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCAt                                                                                                                  <  1:184284/104‑1 (MQ=255)                                              gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                               >  1:1256613/1‑139 (MQ=255)                                              gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                               >  1:1472680/1‑139 (MQ=255)                                                 tggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAtt                                                                            <  2:1256613/139‑1 (MQ=255)                                                   gtGATTCAGGTACCAGAATACTTAAGCCCCGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATtta                                                                          <  1:1077703/139‑1 (MQ=255)                                                        tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                     >  2:862145/1‑139 (MQ=255)                                                           ggTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                                  >  2:198107/1‑139 (MQ=255)                                                                    aTACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATttat                                                                         >  1:1140766/1‑123 (MQ=255)                                                                    aTACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATttat                                                                         <  2:1140766/123‑1 (MQ=255)                                                                       cTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                                 <  2:851617/96‑1 (MQ=255)                                                                       cTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                                 >  1:851617/1‑96 (MQ=255)                                                                             cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                >  2:1173623/1‑75 (MQ=255)                                                                             cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                <  1:1173623/75‑1 (MQ=255)                                                                             cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                              <  2:908718/109‑1 (MQ=255)                                                                             cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                              >  1:908718/1‑109 (MQ=255)                                                                             cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCtt                                                >  1:624534/1‑139 (MQ=255)                                                                              ccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTc                                               <  1:862145/139‑1 (MQ=255)                                                                               cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGa                                                                                                                                  <  2:647547/55‑1 (MQ=255)                                                                               cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGa                                                                                                                                  >  1:647547/1‑55 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                <  2:286160/71‑1 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                >  1:286160/1‑71 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       >  2:448577/1‑80 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       >  1:492559/1‑80 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       <  2:492559/80‑1 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       <  1:448577/80‑1 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                                  <  1:273403/117‑1 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                                  >  2:273403/1‑117 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                                  >  1:68671/1‑133 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                                  <  2:68671/133‑1 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                            >  2:702728/1‑139 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                            >  2:1212092/1‑139 (MQ=255)                                                                                 ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                            >  2:1302105/1‑139 (MQ=255)                                                                                  gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       >  1:1376284/1‑79 (MQ=255)                                                                                  gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       <  2:1376284/79‑1 (MQ=255)                                                                                  gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                              >  2:995766/1‑104 (MQ=255)                                                                                  gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAACGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                              <  1:995766/104‑1 (MQ=255)                                                                                   cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                          <  1:198107/139‑1 (MQ=255)                                                                                   cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                          <  2:624534/139‑1 (MQ=255)                                                                                                   cATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                          <  2:225831/123‑1 (MQ=255)                                                                                                   cATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                          >  1:225831/1‑123 (MQ=255)                                                                                                        cagggaaCGCCGTTTTATGGGGCG‑CCCGAATTACTTTCGGCGCAAGGAAAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCa                    <  1:1027022/135‑1 (MQ=255)                                                                                                          aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCa                                                                                                                         >  2:199552/1‑37 (MQ=255)                                                                                                          aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCa                                                                                                                         <  1:199552/37‑1 (MQ=255)                                                                                                          aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       >  2:1591039/1‑55 (MQ=255)                                                                                                          aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                       <  1:1591039/55‑1 (MQ=255)                                                                                                           agaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                      <  1:464540/55‑1 (MQ=255)                                                                                                           agaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                      >  2:464540/1‑55 (MQ=255)                                                                                                               cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                           <  2:1586045/46‑1 (MQ=255)                                                                                                               cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                           >  1:1586045/1‑46 (MQ=255)                                                                                                               cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGAt                                                                                                     >  1:1631265/1‑52 (MQ=255)                                                                                                               cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGAt                                                                                                     <  2:1631265/52‑1 (MQ=255)                                                                                                               cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCtttt                                                                                  >  1:782250/1‑71 (MQ=255)                                                                                                               cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCtttt                                                                                  <  2:782250/71‑1 (MQ=255)                                                                                                                      atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTtgattgat       <  1:1212092/139‑1 (MQ=255)                                                                                                                       tatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAA‑GGATTGCTCCGGGTTTCCCTTTTTTAAATTTTTATAACAATTCTCTATTTTGGGCTTCCTTAACCCCCTACGCCCTTAATTTTCCCTCGttgatttcttt     >  2:1027022/1‑134 (MQ=255)                                                                                                                           gggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGccc  <  1:702728/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                      GGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCC  >  NC_000913/2265886‑2266146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑