Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,019	G→A	R620C (CGT→TGT)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,019	0	G	A	100.0%	80.8 / NA	26	R620C (CGT→TGT)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

CACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGC  >  NC_000913/2317893‑2318145                                                                                                                               |                                                                                                                               cacaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATa                                                                                                                    >  2:330297/1‑139 (MQ=255)                        gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCa                                                                                             >  1:1514919/1‑139 (MQ=255)                                       cGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCgg                                                                              <  2:1514919/139‑1 (MQ=255)                                          tGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCTAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAt                                                                           >  1:1014505/1‑139 (MQ=255)                                                                agaagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACt                                                     <  2:825140/139‑1 (MQ=255)                                                                         cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGtt                                            >  1:646200/1‑139 (MQ=255)                                                                                 cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                       <  2:539798/72‑1 (MQ=255)                                                                                 cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                       >  1:539798/1‑72 (MQ=255)                                                                                   caccacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGa                                                                                                                              <  1:1660283/47‑1 (MQ=255)                                                                                      cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCAt                                                                                                            <  2:445778/62‑1 (MQ=255)                                                                                      cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCAt                                                                                                            >  1:445778/1‑62 (MQ=255)                                                                                      cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCg                                                                         >  2:831349/1‑97 (MQ=255)                                                                                      cacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCg                                                                         <  1:831349/97‑1 (MQ=255)                                                                                              tCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                       >  2:943938/1‑59 (MQ=255)                                                                                              tCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                       <  1:943938/59‑1 (MQ=255)                                                                                                     tATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                  <  2:1205008/105‑1 (MQ=255)                                                                                                     tATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                  >  1:1205008/1‑105 (MQ=255)                                                                                                      aTCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                    <  2:1344979/54‑1 (MQ=255)                                                                                                      aTCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                    >  1:1344979/1‑54 (MQ=255)                                                                                                                 ggTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaa                                                                                            <  2:1253377/51‑1 (MQ=255)                                                                                                                 ggTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaa                                                                                            >  1:1253377/1‑51 (MQ=255)                                                                                                                   tATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                       >  2:1324145/1‑38 (MQ=255)                                                                                                                   tATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                       <  1:1324145/38‑1 (MQ=255)                                                                                                                   tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                               <  2:1004436/46‑1 (MQ=255)                                                                                                                   tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGc  >  1:955595/1‑139 (MQ=255)                                                                                                                   tATCGCGAACACAGATAGAGAAATAATCGCCATCCGCGCGAACATg                                                                                               >  1:1004436/1‑46 (MQ=38)                                                                                                                               |                                                                                                                               CACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGC  >  NC_000913/2317893‑2318145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑