Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,334,336 C→T pseudogene (67/4605 nt)
*208* (TAG→TAA
yfaS ←
yfaT ←
pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein
DUF1175 family protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,334,3360CT100.0% 173.6 / NA 54pseudogene (67/4605 nt)
*208* (TAG→TAA
yfaS
yfaT
pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein
DUF1175 family protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/28);  total (26/28)

CTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGCTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTC  >  NC_000913/2334209‑2334458
                                                                                                                               |                                                                                                                          
ctgctAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAg                                                                                                                 >  2:793149/1‑139 (MQ=255)
        aCTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTa                                                                                                         <  1:793149/139‑1 (MQ=255)
             tgTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGa                                                                                                    >  1:380594/1‑139 (MQ=255)
              gTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGAt                                                                                                   >  2:709686/1‑139 (MQ=255)
                 agcaagcaAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGa                                                                                                                >  2:1030557/1‑123 (MQ=255)
                 agcaagcaAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTACCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGa                                                                                                                <  1:1030557/123‑1 (MQ=255)
                       caaAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCaa                                                                                          <  2:1420243/139‑1 (MQ=255)
                                  ttCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGa                                                                               <  2:380594/139‑1 (MQ=255)
                                    cccccGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCa                                                                                           <  2:1139922/125‑1 (MQ=255)
                                    cccccGGCAGGCGGCGCATAGTTGCTGCAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCGACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCa                                                                                           >  1:1139922/1‑125 (MQ=255)
                                        cGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGaa                                                                         <  1:238068/139‑1 (MQ=255)
                                        cGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGaa                                                                         >  2:361347/1‑139 (MQ=255)
                                               cggcgCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCACTGAAATTGGGATTg                                                                            >  1:368994/1‑129 (MQ=255)
                                               cggcgCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTg                                                                            <  2:368994/129‑1 (MQ=255)
                                                     aTAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGaa                                                                         <  1:1349101/126‑1 (MQ=255)
                                                     aTAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGaa                                                                         >  2:1349101/1‑126 (MQ=255)
                                                        gTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATc                                                         <  2:1134032/139‑1 (MQ=255)
                                                                aaggaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATc                                                         <  1:171722/131‑1 (MQ=255)
                                                                aaggaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATc                                                         >  2:171722/1‑131 (MQ=255)
                                                                 aggaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCt                                                >  2:1357197/1‑139 (MQ=255)
                                                                   gaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCa                                                                                                                  <  2:1543723/71‑1 (MQ=255)
                                                                   gaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCa                                                                                                                  >  1:1543723/1‑71 (MQ=255)
                                                                   gaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTc                                              <  2:1346913/139‑1 (MQ=255)
                                                                   gaagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTc                                              <  2:769162/139‑1 (MQ=255)
                                                                    aagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGaa                                                                         <  2:1013618/111‑1 (MQ=255)
                                                                    aagCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGaa                                                                         >  1:1013618/1‑111 (MQ=255)
                                                                      gCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGCAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCAt                                           >  1:49561/1‑139 (MQ=255)
                                                                         aaTCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             <  2:971265/134‑1 (MQ=255)
                                                                         aaTCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             >  1:971265/1‑134 (MQ=255)
                                                                          atcatcAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTaaa                                                                                                       <  1:745475/75‑1 (MQ=255)
                                                                          atcatcAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTaaa                                                                                                       >  2:745475/1‑75 (MQ=255)
                                                                            catcaGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATACATCGGGTGTCCTTCCATGTCATa                                     <  1:727666/139‑1 (MQ=255)
                                                                              tcaGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAAtt                                                                                   <  1:780142/91‑1 (MQ=255)
                                                                              tcaGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAAtt                                                                                   >  2:780142/1‑91 (MQ=255)
                                                                                    ttAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGttgttg                             <  2:88993/139‑1 (MQ=255)
                                                                                     tAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             <  1:1572500/122‑1 (MQ=255)
                                                                                     tAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             >  2:1572500/1‑122 (MQ=255)
                                                                                            ccccGTTCCCACAAGAGACAGGGACAACCACGCCGTTTTCGCCGCCGAAAATTTAAAAAATAAAAGCCAAAGAAAAAGGGGTTGGAATCGTTGGGTAACCATCGGGTGTCCTTCCCTTTTCTAAGTTGTTGCAgactga                     >  1:88993/1‑139 (MQ=255)
                                                                                            cccTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCaa                                                                                          <  2:1237431/70‑1 (MQ=255)
                                                                                            cccTGTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCaa                                                                                          >  1:1237431/1‑70 (MQ=255)
                                                                                              cTGTTCACACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAgactgact                   <  1:709686/139‑1 (MQ=255)
                                                                                                gTTCCCACAAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTgc                 >  1:370400/1‑139 (MQ=255)
                                                                                                      acaAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCAt           <  1:1357197/139‑1 (MQ=255)
                                                                                                      acaAGAGACAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCAt           >  2:385492/1‑139 (MQ=255)
                                                                                                           agaCAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             <  2:1359087/100‑1 (MQ=255)
                                                                                                           agaCAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             >  1:1359087/1‑100 (MQ=255)
                                                                                                             aCAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTCTCCATCGGGTGTCCTTCCATGTCATAAg                                   >  1:1011414/1‑108 (MQ=255)
                                                                                                             aCAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAg                                   <  2:1011414/108‑1 (MQ=255)
                                                                                                              cAGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGt   <  1:361347/139‑1 (MQ=255)
                                                                                                               aGGGACAAGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTc  >  1:1201687/1‑139 (MQ=255)
                                                                                                                     aaGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTc                                        >  2:881102/1‑95 (MQ=255)
                                                                                                                     aaGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTc                                        <  1:881102/95‑1 (MQ=255)
                                                                                                                      aGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             <  2:606671/89‑1 (MQ=255)
                                                                                                                      aGCACGCTGTTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTcc                                             >  1:606671/1‑89 (MQ=255)
                                                                                                                            ctgttATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAg                                   >  2:588510/1‑93 (MQ=255)
                                                                                                                            ctgttATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAg                                   <  1:588510/93‑1 (MQ=255)
                                                                                                                            ctgttATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAgactgac                    <  2:47268/108‑1 (MQ=255)
                                                                                                                            ctgttATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAgactgac                    >  1:47268/1‑108 (MQ=255)
                                                                                                                               |                                                                                                                          
CTGCTAAAACTGCTGTCAGCAAGCAAAAAGAATGTTCCCCCGGCAGGCGGCGCATAGTTGCTGGAAGGAAGCGAATCATCAGCATTAGCAAGCCCTGTTCCCACAAGAGACAGGGACAAGCACGCTGCTATCGCGCCAGAAAATTTAAACGATAAATGCCAATGAAATTGGGATTGGAATCGTTGGGTATCCATCGGGTGTCCTTCCATGTCATAAGTTGTTGCAGACTGACTGCGCGCATTCCGTTGTC  >  NC_000913/2334209‑2334458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: