Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,019	T→C	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA → arnD →	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,019	0	T	C	100.0%	179.7 / NA	50	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA arnD	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

AGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAATA  >  NC_000913/2369886‑2370150                                                                                                                                      |                                                                                                                                    agagCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCa                                                                                                                                >  1:1344300/1‑139 (MQ=255) agagCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCa                                                                                                                                >  1:1396756/1‑139 (MQ=255)      aTCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTa                                                                                                                           <  2:1362443/139‑1 (MQ=255)        cGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAgg                                                                                                                         <  2:1396756/139‑1 (MQ=255)          tAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCt                                                                                                                       >  2:435028/1‑139 (MQ=255)                gAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCa                                                                                                                 <  1:219111/139‑1 (MQ=255)                              cccACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGa                                                                                                             >  1:1016542/1‑129 (MQ=255)                              cccACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGa                                                                                                             <  2:1016542/129‑1 (MQ=255)                                cACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCtt                                                                                                 >  2:1388121/1‑139 (MQ=255)                                       gCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                          <  1:1388121/139‑1 (MQ=255)                                        ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTa                                                                                                                     <  2:526914/111‑1 (MQ=255)                                        ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTa                                                                                                                     >  1:526914/1‑111 (MQ=255)                                        ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCa                                                                                         >  1:1427925/1‑139 (MQ=255)                                                 ggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAg                                                                                <  2:1344300/139‑1 (MQ=255)                                                 ggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAg                                                                                <  1:435028/139‑1 (MQ=255)                                                  gAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAgg                                                                               >  1:778557/1‑139 (MQ=255)                                                           aTTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                             >  1:1135764/1‑84 (MQ=255)                                                           aTTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGCATAAACCATCACGACCTAAg                                                                                                                             <  2:1135764/84‑1 (MQ=255)                                                                              tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                   >  2:312387/1‑139 (MQ=255)                                                                              tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                   <  2:1427925/139‑1 (MQ=255)                                                                               cgacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATc                                                         <  1:724583/132‑1 (MQ=255)                                                                               cgacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATc                                                         >  2:724583/1‑132 (MQ=255)                                                                                     aaCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCata                                            <  1:312387/139‑1 (MQ=255)                                                                                     aaCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCata                                            >  1:79059/1‑139 (MQ=255)                                                                                     aaCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCata                                            <  2:663303/139‑1 (MQ=255)                                                                                     aaCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCata                                            <  2:778557/139‑1 (MQ=255)                                                                                            gATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                   <  1:484465/125‑1 (MQ=255)                                                                                            gATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                   >  2:484465/1‑125 (MQ=255)                                                                                                      tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                          >  1:1475045/1‑76 (MQ=255)                                                                                                      tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                          <  2:1475045/76‑1 (MQ=255)                                                                                                                tGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCg                 <  2:79059/139‑1 (MQ=255)                                                                                                                       aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCGGGCCAGCATTTTTTTCAGCg                 >  2:835230/1‑132 (MQ=255)                                                                                                                       aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCGGGCCAGCATTTTTTTCAGCg                 <  1:835230/132‑1 (MQ=255)                                                                                                                        cGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTa                                                  >  2:1048105/1‑98 (MQ=255)                                                                                                                        cGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTa                                                  <  1:1048105/98‑1 (MQ=255)                                                                                                                            tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTctgctg                                                               <  2:283970/81‑1 (MQ=255)                                                                                                                            tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTctgctg                                                               >  1:283970/1‑81 (MQ=255)                                                                                                                            tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGa                                                     <  1:599925/91‑1 (MQ=255)                                                                                                                            tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGa                                                     >  2:599925/1‑91 (MQ=255)                                                                                                                             aaaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACTTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACaa    >  1:1473450/1‑139 (MQ=255)                                                                                                                              aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATa                                                                                                     <  2:152470/41‑1 (MQ=255)                                                                                                                              aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATa                                                                                                     >  1:152470/1‑41 (MQ=255)                                                                                                                              aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                   <  2:368660/91‑1 (MQ=255)                                                                                                                              aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                   >  1:368660/1‑91 (MQ=255)                                                                                                                              aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGc                                  <  1:585220/108‑1 (MQ=255)                                                                                                                              aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGc                                  >  2:585220/1‑108 (MQ=255)                                                                                                                               aCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                 >  2:23605/1‑92 (MQ=255)                                                                                                                               aCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                 <  1:23605/92‑1 (MQ=255)                                                                                                                               aCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAata  >  1:264180/1‑139 (MQ=255)                                                                                                                               aCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAata  >  2:122871/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    AGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAATA  >  NC_000913/2369886‑2370150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑