Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,401,654 G→A C133C (TGC→TGT)  nuoE ← NADH:ubiquinone oxidoreductase, chain E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,401,6540GA100.0% 219.8 / NA 68C133C (TGC→TGT) nuoENADH:ubiquinone oxidoreductase, chain E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/35);  total (33/35)

AGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAA  >  NC_000913/2401518‑2401780
                                                                                                                                        |                                                                                                                              
aGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGaca                                                                                                                              >  1:424876/1‑139 (MQ=255)
    gATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCaa                                                                                                                          <  1:343362/139‑1 (MQ=255)
                   tACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTa                                                                                                           >  1:591360/1‑139 (MQ=255)
                                              cTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCt                                                                                >  1:805365/1‑139 (MQ=255)
                                                      gTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGAt                                                                        >  2:806401/1‑139 (MQ=255)
                                                         cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcagca                                                                                                                <  2:1266754/96‑1 (MQ=255)
                                                         cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcagca                                                                                                                >  1:1266754/1‑96 (MQ=255)
                                                         cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTg                                                                               >  2:689062/1‑129 (MQ=255)
                                                         cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTg                                                                               <  1:689062/129‑1 (MQ=255)
                                                         cAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGtt                                                                     >  2:921974/1‑139 (MQ=255)
                                                                 gCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCgg                                                                                                     >  1:1165134/1‑99 (MQ=255)
                                                                 gCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCgg                                                                                                     <  2:1165134/99‑1 (MQ=255)
                                                                 gCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCtttt                                                             >  2:945119/1‑139 (MQ=255)
                                                                        gggTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAg                                                      <  1:806401/139‑1 (MQ=255)
                                                                             aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcag                                                                                                                  >  1:1032317/1‑74 (MQ=255)
                                                                             aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcag                                                                                                                  <  2:1032317/74‑1 (MQ=255)
                                                                             aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  >  2:928621/1‑106 (MQ=255)
                                                                             aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  <  1:928621/106‑1 (MQ=255)
                                                                             aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg                                                 <  1:921974/139‑1 (MQ=255)
                                                                             aGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg                                                 <  1:1308933/139‑1 (MQ=255)
                                                                                  cgcgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGa                                            <  2:424876/139‑1 (MQ=255)
                                                                                    cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTgg                                                                                                                     >  1:686533/1‑64 (MQ=255)
                                                                                    cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTgg                                                                                                                     <  2:686533/64‑1 (MQ=255)
                                                                                    cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  >  2:1316811/1‑99 (MQ=255)
                                                                                    cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  <  1:1316811/99‑1 (MQ=255)
                                                                                    cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt                                          >  1:479172/1‑139 (MQ=255)
                                                                                    cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt                                          <  2:591360/139‑1 (MQ=255)
                                                                                    cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt                                          >  1:1561403/1‑139 (MQ=255)
                                                                                     gTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACGGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATa                                         <  2:749782/139‑1 (MQ=255)
                                                                                               tCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGACAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAc                               >  2:1636941/1‑139 (MQ=255)
                                                                                                 aTCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCg                             >  2:1515468/1‑139 (MQ=255)
                                                                                                    gATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGACAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTg                          <  1:1636941/139‑1 (MQ=255)
                                                                                                         tcatGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc                                                   <  1:1099504/109‑1 (MQ=255)
                                                                                                         tcatGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc                                                   >  2:1099504/1‑109 (MQ=255)
                                                                                                             gTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa                                           >  1:1082153/1‑113 (MQ=255)
                                                                                                             gTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa                                           <  2:1082153/113‑1 (MQ=255)
                                                                                                                tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGt                            <  2:1383019/125‑1 (MQ=255)
                                                                                                                tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGt                            >  1:1383019/1‑125 (MQ=255)
                                                                                                                  gCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCAc            >  2:269101/1‑139 (MQ=255)
                                                                                                                   cccTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACg           <  1:945119/139‑1 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  <  1:575290/64‑1 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtct                                                          <  1:71976/88‑1 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTtct                                                          >  2:71976/1‑88 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc                                       >  2:211993/1‑107 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc                                       <  1:211993/107‑1 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc                              <  1:27592/116‑1 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc                              >  2:27592/1‑116 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGt       <  2:1561403/139‑1 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGt       <  1:269101/139‑1 (MQ=255)
                                                                                                                       ttATCACAGTTCCCCAGACAGCAAGATGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  >  2:575290/1‑64 (MQ=255)
                                                                                                                        tATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc                                       <  2:928382/106‑1 (MQ=255)
                                                                                                                        tATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc                                       >  1:928382/1‑106 (MQ=255)
                                                                                                                            acaGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACaa  <  2:805365/139‑1 (MQ=255)
                                                                                                                              aGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa                                           >  2:1632932/1‑96 (MQ=255)
                                                                                                                              aGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa                                           <  1:1632932/96‑1 (MQ=255)
                                                                                                                                ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc                                       <  1:1001553/98‑1 (MQ=255)
                                                                                                                                ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAcc                                       >  2:1001553/1‑98 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCTTCAAATGTCGATTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg                                                 >  2:1064406/1‑86 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  >  1:1071322/1‑53 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc                                                                                  <  2:1071322/53‑1 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc                                                   <  1:190415/84‑1 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc                                                   >  2:190415/1‑84 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGcgccg                                                 <  1:1064406/86‑1 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAc                                        <  1:1484015/95‑1 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATAc                                        >  2:1484015/1‑95 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGc          >  2:429134/1‑125 (MQ=255)
                                                                                                                                  ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGc          <  1:429134/125‑1 (MQ=255)
                                                                                                                                   cccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc                              <  2:209584/104‑1 (MQ=255)
                                                                                                                                   cccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc                              >  1:209584/1‑104 (MQ=255)
                                                                                                                                        |                                                                                                                              
AGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAA  >  NC_000913/2401518‑2401780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑