Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,439,222 G→A P300L (CCA→CTA)  yfcJ ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,439,2220GA100.0% 87.9 / NA 29P300L (CCA→CTA) yfcJputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

CGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCA  >  NC_000913/2439088‑2439347
                                                                                                                                      |                                                                                                                             
cggcgTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGc                                                                                                                           >  2:1246842/1‑139 (MQ=255)
   cgTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTg                                                                                                                        >  1:40542/1‑139 (MQ=255)
                 gCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGcaaca                                                                                                          <  1:1246842/139‑1 (MQ=255)
                          cgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCg                                                                                                 >  2:580114/1‑139 (MQ=255)
                          cgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCg                                                                                                 <  2:574335/139‑1 (MQ=255)
                                                         ccACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGcc                                                                                                      <  1:50921/103‑1 (MQ=255)
                                                         ccACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGcc                                                                                                      >  2:50921/1‑103 (MQ=255)
                                                             gCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAg                                                                                       <  1:1591615/114‑1 (MQ=255)
                                                             gCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAg                                                                                       >  2:1591615/1‑114 (MQ=255)
                                                                 aGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGc                                                                                                                       >  1:50710/1‑78 (MQ=255)
                                                                 aGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGc                                                                                                                       <  2:50710/78‑1 (MQ=255)
                                                                   cgcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGcc                                                                                                                      <  2:536864/77‑1 (MQ=255)
                                                                   cgcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGcc                                                                                                                      >  1:536864/1‑77 (MQ=255)
                                                                    gcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCaa                                                       >  2:872210/1‑139 (MQ=255)
                                                                    gcAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCaa                                                       <  1:872210/139‑1 (MQ=255)
                                                                       ggAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCa                                                                                                                     >  2:1173363/1‑74 (MQ=255)
                                                                       ggAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCa                                                                                                                     <  1:1173363/74‑1 (MQ=255)
                                                                                  cGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGcaa                                                                                                            <  1:618436/72‑1 (MQ=255)
                                                                                  cGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGcaa                                                                                                            >  2:618436/1‑72 (MQ=255)
                                                                                  cGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATc                                         <  2:301710/139‑1 (MQ=255)
                                                                                              tCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGTAACAAGCCCa                                                                                                    >  2:266435/1‑68 (MQ=255)
                                                                                              tCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGTAACAAGCCCa                                                                                                    <  1:266435/68‑1 (MQ=255)
                                                                                                  gTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACa                                                                                         >  2:419432/1‑75 (MQ=255)
                                                                                                  gTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACa                                                                                         <  1:419432/75‑1 (MQ=255)
                                                                                                          cgcgCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCagagag                                                                                >  2:1291896/1‑76 (MQ=255)
                                                                                                          cgcgCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCagagag                                                                                <  1:1291896/76‑1 (MQ=255)
                                                                                                          cgcgCCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACg                 >  1:257093/1‑139 (MQ=255)
                                                                                                            cgcCCGCTAATGCGACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGAc               >  2:955778/1‑139 (MQ=255)
                                                                                                                         gACCCATGCACCTAGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCTCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCTCGACAAATGCGCCGCCa  <  1:955778/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                             
CGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCGCATCACGACAAATGCGCCGCCA  >  NC_000913/2439088‑2439347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: