Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,228 C→T *274* (TAG→TAA yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2280CT100.0% 87.4 / NA 28*274* (TAG→TAAyfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/16);  total (12/16)

TGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACC  >  NC_000913/2449097‑2449354
                                                                                                                                   |                                                                                                                              
tGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACt                                                                                                                         >  1:512954/1‑139 (MQ=255)
    gcggcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAtt                                                                                                                     <  1:1533101/139‑1 (MQ=255)
     cggcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTc                                                                                                                    >  1:1113075/1‑139 (MQ=255)
                        tgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGc                                                                                                 <  2:1303292/139‑1 (MQ=255)
                                        cTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                 <  2:1113075/139‑1 (MQ=255)
                                                    ttGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATActgct                                                                     <  1:1339970/139‑1 (MQ=255)
                                                       cccTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                  <  2:902593/139‑1 (MQ=255)
                                                       cccTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                  <  2:854412/139‑1 (MQ=255)
                                                            aggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCaa                                                             <  2:512954/139‑1 (MQ=255)
                                                              gagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAaca                                                           >  2:894909/1‑139 (MQ=255)
                                                                gTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                         <  2:125345/139‑1 (MQ=255)
                                                                                        ggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCa                                                                                                                   >  1:1290346/1‑57 (MQ=255)
                                                                                        ggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCa                                                                                                                   <  2:1290346/57‑1 (MQ=255)
                                                                                                   gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAAc                                                                                                                          >  1:150625/1‑39 (MQ=255)
                                                                                                   gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAAc                                                                                                                          <  2:150625/39‑1 (MQ=255)
                                                                                                                       tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa                                                                          >  1:790224/1‑67 (MQ=255)
                                                                                                                       tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa                                                                          <  2:790224/67‑1 (MQ=255)
                                                                                                                       tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                  >  1:1654836/1‑75 (MQ=255)
                                                                                                                       tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                  <  2:1654836/75‑1 (MQ=255)
                                                                                                                       tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGAcc  >  1:25333/1‑139 (MQ=255)
                                                                                                                        gtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGAc                                                   >  2:647104/1‑89 (MQ=255)
                                                                                                                        gtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGAc                                                   <  1:647104/89‑1 (MQ=255)
                                                                                                                           tCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                         >  2:1618574/1‑80 (MQ=255)
                                                                                                                           tCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                         <  1:1618574/80‑1 (MQ=255)
                                                                                                                            cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa                                                                            >  2:585720/1‑60 (MQ=255)
                                                                                                                            cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa                                                                            <  1:585720/60‑1 (MQ=255)
                                                                                                                                gggTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa                                                                            >  2:1612375/1‑56 (MQ=255)
                                                                                                                                gggTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa                                                                            <  1:1612375/56‑1 (MQ=255)
                                                                                                                                   |                                                                                                                              
TGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACC  >  NC_000913/2449097‑2449354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: