Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,471,105 G→A pseudogene (291/291 nt)
P138L (CCT→CTT) 
tfaS →
yfdK ←
pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related
CPS‑53 (KpLE1) prophage, conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,471,1050GA100.0% 158.4 / NA 50pseudogene (291/291 nt)
P138L (CCT→CTT) 
tfaS
yfdK
pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related
CPS‑53 (KpLE1) prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

CTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACT  >  NC_000913/2470978‑2471238
                                                                                                                               |                                                                                                                                     
cTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAata                                                                                                                            >  1:309445/1‑139 (MQ=255)
 tGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAatat                                                                                                                           >  2:953138/1‑139 (MQ=255)
             gATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTgg                                                                                                               <  1:953138/139‑1 (MQ=255)
               tGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGaa                                                                                                             >  1:1217738/1‑139 (MQ=255)
                    gcagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATc                                                                                                        >  1:252888/1‑139 (MQ=255)
                          aCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAg                                                                                                  <  2:408313/139‑1 (MQ=255)
                            cTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCt                                                                                                >  2:43032/1‑139 (MQ=255)
                              cccGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTcc                                                                                              >  1:813652/1‑139 (MQ=255)
                                cGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                            <  2:866374/139‑1 (MQ=255)
                                 gACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                           <  2:813652/139‑1 (MQ=255)
                                     tAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGt                                                                                       <  2:309445/139‑1 (MQ=255)
                                             tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCGGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                     <  1:1184649/117‑1 (MQ=255)
                                             tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                                     >  2:1184649/1‑117 (MQ=255)
                                             tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATaa                                                                               >  2:1429958/1‑139 (MQ=255)
                                             tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATaa                                                                               >  1:989888/1‑139 (MQ=255)
                                               cTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATc                                                                             <  1:1429958/139‑1 (MQ=255)
                                                tGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATcc                                                                            <  1:43032/139‑1 (MQ=255)
                                                  gATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                          <  1:952839/139‑1 (MQ=255)
                                                      aCATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCcac                                                                      <  2:252888/139‑1 (MQ=255)
                                                       cATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                         <  2:1293959/103‑1 (MQ=38)
                                                              gcgGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATtata                                                              >  2:91079/1‑139 (MQ=255)
                                                                    aCGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                        <  1:1347620/123‑1 (MQ=255)
                                                                    aCGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                        >  2:1347620/1‑123 (MQ=255)
                                                                         aaCAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGtt                                                   <  2:1217738/139‑1 (MQ=255)
                                                                                aCCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCAcc                                            >  1:1643126/1‑139 (MQ=255)
                                                                                     cACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCa                                       >  1:1096829/1‑139 (MQ=255)
                                                                                        cgcgCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAgac                                    <  2:989888/139‑1 (MQ=255)
                                                                                          cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                           >  2:39436/1‑82 (MQ=255)
                                                                                          cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                           <  1:39436/82‑1 (MQ=255)
                                                                                          cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                        <  1:155779/101‑1 (MQ=255)
                                                                                          cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                        >  2:155779/1‑101 (MQ=255)
                                                                                          cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAgacga                                  >  2:531157/1‑139 (MQ=255)
                                                                                            ccGGATGTCATCTGGCCTGAACTGCCGTAGGAGTAAGCCATTCAATATCTGGCTCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                          <  1:1644577/97‑1 (MQ=255)
                                                                                            ccGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                          >  2:1644577/1‑97 (MQ=255)
                                                                                                aTGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATa                            <  1:91079/139‑1 (MQ=255)
                                                                                                         ggCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCg                        <  1:671478/134‑1 (MQ=255)
                                                                                                         ggCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCg                        >  2:671478/1‑134 (MQ=255)
                                                                                                           ccTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTAcc                 >  2:999036/1‑139 (MQ=255)
                                                                                                            cTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCa                <  1:999036/139‑1 (MQ=255)
                                                                                                             tGAACTGCCGGAGGAGTAAGCCATTCAATATCTGgcg                                                                                                                     <  1:1096785/37‑1 (MQ=14)
                                                                                                             tGAACTGCCGGAGGAGTAAGCCATTCAATATCTGgcg                                                                                                                     >  2:1096785/1‑37 (MQ=255)
                                                                                                             tGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGa                                                                                  >  1:1188781/1‑72 (MQ=255)
                                                                                                             tGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGa                                                                                  <  2:1188781/72‑1 (MQ=255)
                                                                                                             tGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                        <  1:510993/82‑1 (MQ=255)
                                                                                                             tGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                        >  2:510993/1‑82 (MQ=255)
                                                                                                                    ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                          >  1:565011/1‑73 (MQ=255)
                                                                                                                    ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                          <  2:565011/73‑1 (MQ=255)
                                                                                                                    ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTg        >  2:5980/1‑139 (MQ=255)
                                                                                                                          gagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACt  <  2:1643126/139‑1 (MQ=255)
                                                                                                                            gTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGa                                                                                  >  1:1492479/1‑57 (MQ=255)
                                                                                                                               |                                                                                                                                     
CTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACT  >  NC_000913/2470978‑2471238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: