Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,574,135 G→A D57D (GAC→GAT)  eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,574,1350GA100.0% 130.4 / NA 42D57D (GAC→GAT) eutTcobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

TGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGT  >  NC_000913/2574019‑2574263
                                                                                                                    |                                                                                                                                
tGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                            <  1:1271247/139‑1 (MQ=255)
        cGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaaca                                                                                                                            >  2:1474384/1‑115 (MQ=255)
        cGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaaca                                                                                                                            <  1:1474384/115‑1 (MQ=255)
         gTATCCGGCTTTTTTGACATCGGCTGGCGACACAGTTCGCAGCCCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGATCATCATCAACAAACAGGCGGCCCTGCTCGTCAATaa                                                                                                   >  1:1164273/1‑139 (MQ=255)
                 cTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaac                                                                                                                             <  1:1136990/105‑1 (MQ=255)
                 cTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaac                                                                                                                             >  2:1136990/1‑105 (MQ=255)
                           aCCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggc                                                                                 <  1:1573655/139‑1 (MQ=255)
                           aCCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATggc                                                                                 >  1:222233/1‑139 (MQ=255)
                                   gCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTcc                                                                         >  2:245397/1‑139 (MQ=255)
                                      acacAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                      <  2:222233/139‑1 (MQ=255)
                                            ttCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTcc                                                                <  1:245397/139‑1 (MQ=255)
                                                    aCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCaga                                                        >  1:119277/1‑139 (MQ=255)
                                                    aCGCCTGCGGATGTTCATCGCTACTGGGCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCaga                                                        >  1:998257/1‑139 (MQ=255)
                                                     cGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                              <  2:1085200/84‑1 (MQ=255)
                                                     cGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                              >  1:1085200/1‑84 (MQ=255)
                                                      gCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAgg                                                      >  1:1151267/1‑139 (MQ=255)
                                                                 ttCATCGCTACTGGTCAGCCCATGAACGGGCTGCTGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGa                                           <  2:1164273/139‑1 (MQ=255)
                                                                  tCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                              >  2:603417/1‑71 (MQ=255)
                                                                  tCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                              <  1:603417/71‑1 (MQ=255)
                                                                           cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCag                                                         >  1:12217/1‑115 (MQ=255)
                                                                           cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCag                                                         <  2:12217/115‑1 (MQ=255)
                                                                           cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg                                                     <  1:950359/119‑1 (MQ=255)
                                                                           cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg                                                     >  2:123260/1‑119 (MQ=255)
                                                                           cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg                                                     >  2:950359/1‑119 (MQ=255)
                                                                           cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg                                                     <  1:123260/119‑1 (MQ=255)
                                                                           cTGGTCAGCCCATGAACGGGCTGCGGCGGCTGCTGTCCACCATCAACAAACAGGCGGCCCTGCTCGTCAATAAGCGTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                 <  1:978771/139‑1 (MQ=255)
                                                                                   cccATGAACGGGCTGCGGCTGTTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCa                                  >  1:407170/1‑130 (MQ=255)
                                                                                   cccATGAACGGGCTGCGGCTGTTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCa                                  <  2:407170/130‑1 (MQ=255)
                                                                                   cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg                                                                                                                      <  1:7480/46‑1 (MQ=255)
                                                                                   cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg                                                                                                                      >  2:7480/1‑46 (MQ=255)
                                                                                   cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCttt                                                                           >  1:42234/1‑89 (MQ=255)
                                                                                   cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCttt                                                                           <  2:42234/89‑1 (MQ=255)
                                                                                        gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCg                                    <  1:541641/123‑1 (MQ=255)
                                                                                        gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCg                                    >  2:541641/1‑123 (MQ=255)
                                                                                             ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGa                        <  2:384086/130‑1 (MQ=255)
                                                                                             ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGa                        >  1:384086/1‑130 (MQ=255)
                                                                                                        ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                           <  1:376169/36‑1 (MQ=255)
                                                                                                        ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                           >  2:376169/1‑36 (MQ=255)
                                                                                                          gctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGt  <  2:998257/139‑1 (MQ=255)
                                                                                                          gctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGt  <  2:119277/139‑1 (MQ=255)
                                                                                                             gTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTcc                                                                         <  2:961682/65‑1 (MQ=255)
                                                                                                             gTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTcc                                                                         >  1:961682/1‑65 (MQ=255)
                                                                                                                catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGAtc                      <  2:616845/113‑1 (MQ=255)
                                                                                                                catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGAtc                      >  1:616845/1‑113 (MQ=255)
                                                                                                                    |                                                                                                                                
TGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGT  >  NC_000913/2574019‑2574263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑