Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,589,535 C→T I647I (ATC→ATT)  acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,589,5350CT100.0% 214.3 / NA 66I647I (ATC→ATT) acrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/35);  total (31/35)

AGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGAT  >  NC_000913/2589400‑2589670
                                                                                                                                       |                                                                                                                                       
aGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCCCGTTTGCCATTATTGAg                                                                                                                                      <  1:955917/139‑1 (MQ=255)
      catcatGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAg                                                                                                                                      <  2:876353/133‑1 (MQ=255)
      catcatGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAg                                                                                                                                      >  1:876353/1‑133 (MQ=255)
           tGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGaa                                                                                                                           >  2:747866/1‑139 (MQ=255)
           tGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGaa                                                                                                                           <  1:1503593/139‑1 (MQ=255)
                 tgtTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCg                                                                                                                                    <  1:1112856/124‑1 (MQ=255)
                 tgtTTGCCACCGTTGGTTCAGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCg                                                                                                                                    >  2:1112856/1‑124 (MQ=255)
                    ttGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTaa                                                                                                                  <  1:18225/139‑1 (MQ=255)
                      gCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAcc                                                                                                                >  1:1596600/1‑139 (MQ=255)
                       ccACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCa                                                                                                               <  1:747866/139‑1 (MQ=255)
                           cGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAAtt                                                                                                           >  1:1506140/1‑139 (MQ=255)
                               ggTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAaag                                                                                                       >  1:1463030/1‑139 (MQ=255)
                                     ggCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 <  1:46686/123‑1 (MQ=255)
                                     ggCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 >  2:46686/1‑123 (MQ=255)
                                     ggCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                                                 <  2:1463030/139‑1 (MQ=255)
                                          tGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGtt                                                                                            >  1:1547778/1‑139 (MQ=255)
                                           gggggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGt                                                                                                                                   <  1:892075/99‑1 (MQ=255)
                                           gggggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGt                                                                                                                                   >  2:892075/1‑99 (MQ=255)
                                           gggggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 <  2:1433215/117‑1 (MQ=255)
                                           gggggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 >  1:1433215/1‑117 (MQ=255)
                                           gggggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTa                                                                                           >  1:1226902/1‑139 (MQ=255)
                                                 aaCGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 <  2:1503248/111‑1 (MQ=255)
                                                 aaCGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 >  1:1503248/1‑111 (MQ=255)
                                                 aaCGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCca                                                                                     >  1:501601/1‑139 (MQ=255)
                                                      gCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCg                                                                                                                                    >  1:1417899/1‑87 (MQ=255)
                                                      gCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCg                                                                                                                                    <  2:1417899/87‑1 (MQ=255)
                                                      gCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCagcagc                                                                                >  2:367431/1‑139 (MQ=255)
                                                      gCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCagcagc                                                                                >  1:356850/1‑139 (MQ=255)
                                                           aCGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGcc                                                                           <  2:356850/139‑1 (MQ=255)
                                                            cGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCa                                                                          <  2:1506140/139‑1 (MQ=255)
                                                             gTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAg                                                                         <  2:1547778/139‑1 (MQ=255)
                                                                gcgcGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCaa                                                                      >  1:1241661/1‑139 (MQ=255)
                                                                   cgAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTa                                                                   <  2:38470/139‑1 (MQ=255)
                                                                      aTGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACACTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCg                                                                <  1:1066595/139‑1 (MQ=255)
                                                                        gTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGt                                                              <  2:1241661/139‑1 (MQ=255)
                                                                           tATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCtt                                                           <  1:367431/139‑1 (MQ=255)
                                                                                 ccTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 >  1:84380/1‑79 (MQ=255)
                                                                                 ccTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 <  2:84380/79‑1 (MQ=255)
                                                                                 ccTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTCGGTAGt                                                     >  1:291511/1‑139 (MQ=255)
                                                                                  cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                                                 <  2:13715/94‑1 (MQ=255)
                                                                                  cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTc                                                                                                 >  1:13715/1‑94 (MQ=255)
                                                                                  cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGcc                                                                           <  1:1479425/116‑1 (MQ=255)
                                                                                  cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGcc                                                                           >  2:1479425/1‑116 (MQ=255)
                                                                                  cTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGtt                                                    >  2:1559166/1‑139 (MQ=255)
                                                                                     aaaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTg                                                 <  1:1559166/139‑1 (MQ=255)
                                                                                     aaaGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTg                                                 <  2:1226902/139‑1 (MQ=255)
                                                                                               gCGAACGCGACAGTAAGACCGGCACCTCGTTCGCCATTATTGAGCGTGCAACGaa                                                                                                                           <  1:238650/55‑1 (MQ=255)
                                                                                               gCGAACGCGACAGTAAGACCGGCACCTCGTTCGCCATTATTGAGCGTGCAACGaa                                                                                                                           >  2:238650/1‑55 (MQ=255)
                                                                                                  aaCGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAaag                                                                                                       >  1:470726/1‑72 (MQ=255)
                                                                                                  aaCGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAaag                                                                                                       <  2:470726/72‑1 (MQ=255)
                                                                                                  aaCGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTg                                                 >  2:303893/1‑126 (MQ=255)
                                                                                                  aaCGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTg                                                 <  1:303893/126‑1 (MQ=255)
                                                                                                   aCGCGACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATg                                   >  1:1499441/1‑139 (MQ=255)
                                                                                                    cgcgACAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATgg                                  <  2:501601/139‑1 (MQ=255)
                                                                                                         cAGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTg                             <  2:1297429/139‑1 (MQ=255)
                                                                                                          aGTAAGACCGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGc                            <  2:1575726/139‑1 (MQ=255)
                                                                                                                  cGGCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGCCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCAc                    >  1:404137/1‑139 (MQ=255)
                                                                                                                   ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGata                                     <  1:751023/121‑1 (MQ=255)
                                                                                                                   ggCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGata                                     >  2:751023/1‑121 (MQ=255)
                                                                                                                    gCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGtt                                                                                            <  1:1076121/65‑1 (MQ=255)
                                                                                                                    gCACCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGtt                                                                                            >  2:1076121/1‑65 (MQ=255)
                                                                                                                      aCCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 <  1:1241817/42‑1 (MQ=255)
                                                                                                                      aCCTCGTTTGCCATTATTGAGCGTGCAACGAAGGCGTTTAAc                                                                                                                 >  2:1241817/1‑42 (MQ=255)
                                                                                                                                   ttATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTa                                                                                           >  2:1666233/1‑51 (MQ=255)
                                                                                                                                   ttATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTa                                                                                           <  1:1666233/51‑1 (MQ=255)
                                                                                                                                    tATTGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGAt  <  2:404137/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGCAGCCCGCCAGCAATTAGCGGTCTTGGTAGTTCTGCAGGTTTTGATATGGAGTTGCAGGACCACGCTGGAGCGGGTCACGAT  >  NC_000913/2589400‑2589670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑