Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,591,603	G→A	*119* (TAG→TAA)	yffB →	putative ArsC family reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,591,603	0	G	A	100.0%	213.2 / NA	67	*119* (TAG→TAA)	yffB	putative ArsC family reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/36);  total (31/36)

CTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCATG  >  NC_000913/2591479‑2591735                                                                                                                             |                                                                                                                                     ctGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGccc                                                                                                                        <  1:1172728/139‑1 (MQ=255)    cggcggCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGt                                                                                                                     >  2:294246/1‑139 (MQ=255)          cATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               <  2:1141631/139‑1 (MQ=255)          cATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               <  1:1270864/139‑1 (MQ=255)            ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAt                                                                                                                  <  2:1655103/134‑1 (MQ=255)            ttAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTAt                                                                                                                  >  1:1655103/1‑134 (MQ=255)                  aCTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGacac                                                                                                       <  1:294246/139‑1 (MQ=255)                          gCCTGCAATCATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCtt                                                                                               <  1:984399/139‑1 (MQ=255)                           ccTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               >  2:1553729/1‑122 (MQ=255)                           ccTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               <  1:1553729/122‑1 (MQ=255)                            cTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTAt                                                                                             >  1:211033/1‑139 (MQ=255)                             tGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTAtt                                                                                            >  1:1517792/1‑139 (MQ=255)                                     tCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGccc                                                                                    <  2:211033/139‑1 (MQ=255)                                     tCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCAGGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGccc                                                                                    >  1:1012096/1‑139 (MQ=255)                                         aCGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTcc                                                                                <  2:610925/139‑1 (MQ=255)                                         aCGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTcc                                                                                <  2:1012096/139‑1 (MQ=255)                                         aCGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTcc                                                                                <  1:430135/139‑1 (MQ=255)                                                      tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccgc                                                                                      >  2:1275044/1‑120 (MQ=255)                                                      tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgccgc                                                                                      <  1:1275044/120‑1 (MQ=255)                                                      tGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtgatg                                                                   >  2:418515/1‑139 (MQ=255)                                                        cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               <  1:144861/93‑1 (MQ=255)                                                        cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               >  2:144861/1‑93 (MQ=255)                                                        cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                                     <  2:1383757/135‑1 (MQ=255)                                                        cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                                     >  1:819984/1‑135 (MQ=255)                                                        cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                                     <  2:819984/135‑1 (MQ=255)                                                        cGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                                     >  1:1383757/1‑135 (MQ=255)                                                             ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgcc                                                                                        >  1:426104/1‑111 (MQ=255)                                                             ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTcgcc                                                                                        <  2:426104/111‑1 (MQ=255)                                                             ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                          <  1:743331/125‑1 (MQ=255)                                                             ccGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTAACAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                          >  2:743331/1‑125 (MQ=255)                                                                gTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATg                                                         <  1:38891/139‑1 (MQ=255)                                                                  aaGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGcc                                                       >  2:3728/1‑139 (MQ=255)                                                                       tATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCt                                                  <  1:97554/139‑1 (MQ=255)                                                                         tgctgcTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                          <  2:177167/113‑1 (MQ=255)                                                                         tgctgcTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                          >  1:177167/1‑113 (MQ=255)                                                                          gctgctGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTTCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTttg                                               <  2:191078/139‑1 (MQ=255)                                                                           ctgctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTcc                                                                                >  1:471029/1‑105 (MQ=255)                                                                           ctgctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTcc                                                                                <  2:471029/105‑1 (MQ=255)                                                                              ctgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGttga                                           >  1:1345531/1‑139 (MQ=255)                                                                               tgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               <  2:908538/70‑1 (MQ=255)                                                                               tgGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               >  1:908538/1‑70 (MQ=255)                                                                                   tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               <  1:283171/66‑1 (MQ=255)                                                                                   tttCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGa                                                                                                               >  2:283171/1‑66 (MQ=255)                                                                                             tCCAGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAgg                            >  2:1003631/1‑139 (MQ=255)                                                                                                aGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                          >  2:1418009/1‑90 (MQ=255)                                                                                                aGTTATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGt                                                                          <  1:1418009/90‑1 (MQ=255)                                                                                                  ttATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATc                       <  1:3728/139‑1 (MQ=255)                                                                                                   tATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATg                                                         <  2:1322489/104‑1 (MQ=255)                                                                                                   tATCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATg                                                         >  1:1322489/1‑104 (MQ=255)                                                                                                     tCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGccc                                                                                                                        <  2:122888/39‑1 (MQ=255)                                                                                                     tCAGCAATTTTTCCATGAGGTGTAATCTATGTCGTGccc                                                                                                                        >  1:122888/1‑39 (MQ=255)                                                                                                       aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTccc                                                                               <  2:421372/78‑1 (MQ=255)                                                                                                       aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTccc                                                                               >  1:421372/1‑78 (MQ=255)                                                                                                       aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGttt                  >  1:1566602/1‑139 (MQ=255)                                                                                                       aGCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGttt                  >  1:314718/1‑139 (MQ=255)                                                                                                        gCAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGtttt                 <  2:1345531/139‑1 (MQ=255)                                                                                                         cAATTTTTCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTa                <  2:1517792/139‑1 (MQ=255)                                                                                                            tttttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                                     >  1:844760/1‑67 (MQ=255)                                                                                                            tttttCCATGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                                     <  2:844760/67‑1 (MQ=255)                                                                                                                   aTGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCtga                                                                     <  1:1128829/76‑1 (MQ=255)                                                                                                                   aTGAGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATGCGCCGCCCTTCCCTGAGTCCtga                                                                     >  2:1128829/1‑76 (MQ=255)                                                                                                                      aGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCtt                                                 <  2:620994/93‑1 (MQ=255)                                                                                                                      aGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCtt                                                 >  1:620994/1‑93 (MQ=255)                                                                                                                      aGGTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTAGAACGCAt   >  2:991050/1‑139 (MQ=255)                                                                                                                       ggTGTAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCATg  <  1:418515/139‑1 (MQ=255)                                                                                                                        gtgtAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                                     >  2:1371144/1‑55 (MQ=255)                                                                                                                        gtgtAATCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCgccgcc                                                                                     <  1:1371144/55‑1 (MQ=255)                                                                                                                             |                                                                                                                                     CTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGATGCAGGATGCCAGGCTTTGTTGATTGAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCATG  >  NC_000913/2591479‑2591735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑