Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,697,915	C→T	*212* (TAG→TAA)	pgpC ←	phosphatidylglycerophosphatase C, membrane bound

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,697,915	0	C	T	100.0%	190.9 / NA	59	*212* (TAG→TAA)	pgpC	phosphatidylglycerophosphatase C, membrane bound
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

CCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCG  >  NC_000913/2697788‑2698043                                                                                                                                |                                                                                                                                 cccAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTg                                                                                                                       <  1:842993/139‑1 (MQ=255)           tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTg                                                                                                                       >  2:1656877/1‑129 (MQ=255)           tttCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTg                                                                                                                       <  1:1656877/129‑1 (MQ=255)              cGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcg                                                                                                          <  2:59049/139‑1 (MQ=255)                ccAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                        <  1:1119997/139‑1 (MQ=255)                        aGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCGGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATGATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa                                                                                                <  1:297555/139‑1 (MQ=255)                             gTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGc                                                                                           >  2:786136/1‑139 (MQ=255)                              tGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                        <  2:570089/125‑1 (MQ=255)                              tGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                        >  1:570089/1‑125 (MQ=255)                                    aTCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGAt                                                                                    >  2:768046/1‑139 (MQ=255)                                    aTCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGAt                                                                                    <  1:786136/139‑1 (MQ=255)                                          tATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGAc                                                                              >  2:975366/1‑139 (MQ=255)                                            ttCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGc                                                                                           >  1:777045/1‑124 (MQ=255)                                            ttCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGc                                                                                           <  2:777045/124‑1 (MQ=255)                                               gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                                  >  2:1301844/1‑114 (MQ=255)                                               gTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGt                                                                                                  <  1:1301844/114‑1 (MQ=255)                                                tGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAAt                                                                        >  2:347467/1‑139 (MQ=255)                                                tGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAAt                                                                        >  1:292551/1‑139 (MQ=255)                                                  gCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATaa                                                                      >  1:906451/1‑139 (MQ=255)                                                   cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCa                                                                                                                           <  2:1387952/85‑1 (MQ=255)                                                   cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCa                                                                                                                           >  1:1387952/1‑85 (MQ=255)                                                   cTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATaaa                                                                     >  2:354386/1‑139 (MQ=255)                                                    tAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                        >  2:378723/1‑103 (MQ=255)                                                    tAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACcgcg                                                                                                        <  1:378723/103‑1 (MQ=255)                                                      aaTTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAagca                                                                  <  1:347467/139‑1 (MQ=255)                                                      aaTTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAagca                                                                  <  2:906451/139‑1 (MQ=255)                                                             cTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                           <  2:1643267/139‑1 (MQ=255)                                                                       aaaGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTa                                                 >  2:752763/1‑139 (MQ=255)                                                                          gAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGTATTGTCCTGATTACTg                                              <  1:354386/139‑1 (MQ=255)                                                                          gAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTg                                              <  1:768046/139‑1 (MQ=255)                                                                             aaCTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGt                                                                                                                         <  1:621722/61‑1 (MQ=255)                                                                             aaCTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGt                                                                                                                         >  2:621722/1‑61 (MQ=255)                                                                                   ggTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTg                                              >  1:1668377/1‑130 (MQ=255)                                                                                   ggTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTg                                              <  2:1668377/130‑1 (MQ=255)                                                                                   ggTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAg                                     >  2:1271869/1‑139 (MQ=255)                                                                                              cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCtat                                       <  1:1138648/126‑1 (MQ=255)                                                                                              cgcgGCGCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCtat                                       >  2:1138648/1‑126 (MQ=255)                                                                                               gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa                                                                                                >  2:1370991/1‑68 (MQ=255)                                                                                               gcggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTa                                                                                                <  1:1370991/68‑1 (MQ=255)                                                                                                cggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                           <  2:936367/104‑1 (MQ=255)                                                                                                cggcgCATTATACACGGACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGAtt                                                           >  1:936367/1‑104 (MQ=255)                                                                                                               ggACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGcc                                   >  2:472702/1‑113 (MQ=255)                                                                                                               ggACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGcc                                   <  1:472702/113‑1 (MQ=255)                                                                                                                gACGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGAt        >  1:491811/1‑139 (MQ=255)                                                                                                                 aCGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCt                                         <  2:73640/105‑1 (MQ=255)                                                                                                                 aCGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCt                                         >  1:73640/1‑105 (MQ=255)                                                                                                                  cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGGAATAAagcagc                                                                >  2:976231/1‑81 (MQ=255)                                                                                                                  cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGt                                                         >  2:381946/1‑88 (MQ=255)                                                                                                                  cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGt                                                         <  1:381946/88‑1 (MQ=255)                                                                                                                  cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGc                                    >  2:670752/1‑109 (MQ=255)                                                                                                                  cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGc                                    <  2:1356093/109‑1 (MQ=255)                                                                                                                  cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGc                                    <  1:670752/109‑1 (MQ=255)                                                                                                                  cGCTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGc                                    >  1:1356093/1‑109 (MQ=255)                                                                                                                  cGCTATGCTTTACCTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGGAATAAagcagc                                                                <  1:976231/81‑1 (MQ=255)                                                                                                                    cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                             <  2:1283159/82‑1 (MQ=255)                                                                                                                    cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGa                                                             >  1:1283159/1‑82 (MQ=255)                                                                                                                    cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTg    <  1:975366/139‑1 (MQ=255)                                                                                                                    cTATGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTg    <  1:752763/139‑1 (MQ=255)                                                                                                                      aTGCTTTACTTTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTgcg  >  2:1613727/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                 CCCAGGCACGTTTCGCCAGCGTCAGCGCGTGACGCATCCAGTATTCGTGGCTAAATTCGACTTCAGACAAAAAGAAAACTCCGGTTATAAAAGCGCGGCGCATTATACACGGACGCTATGCTTTACTCTATTCCAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCG  >  NC_000913/2697788‑2698043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑