Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,760,667 G→A D41N (GAC→AAC)  yfjJ → CP4‑57 prophage, uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,760,6670GA100.0% 206.3 / NA 63D41N (GAC→AAC) yfjJCP4‑57 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/31);  total (32/31)

TTGGCCAGGATACTATATGACATACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATGACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAACATGATAAGCATATTAAAACTCAACGGAAAGACT  >  NC_000913/2760531‑2760802
                                                                                                                                        |                                                                                                                                       
ttGGCCAGGATACTATATGACATACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAAc                                                                                                                                       >  1:1045555/1‑139 (MQ=255)
             tataTGACATACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAg                                                                                                                          >  2:15458/1‑139 (MQ=255)
                 tGACATACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGaaaa                                                                                                                      >  1:1461145/1‑139 (MQ=255)
                  gACATACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAat                                                                                                                     <  1:644402/139‑1 (MQ=255)
                      tACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATgg                                                                                                                 >  1:921488/1‑139 (MQ=255)
                       aCGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGa                                                                                                                >  1:1095880/1‑139 (MQ=255)
                       aCGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGa                                                                                                                >  2:953964/1‑139 (MQ=255)
                              tGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCa                                                                                                         >  2:526447/1‑139 (MQ=255)
                                        tCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAAc                                                                                               <  2:1461145/139‑1 (MQ=255)
                                         cacaTGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAAcc                                                                                              <  1:526447/139‑1 (MQ=255)
                                         cacaTGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAAcc                                                                                              <  2:921488/139‑1 (MQ=255)
                                                aTCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGtt                                                                                       <  1:15458/139‑1 (MQ=255)
                                                 tCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTg                                                                                      >  2:260938/1‑139 (MQ=255)
                                                     tataTCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGActc                                                                                  <  1:260938/139‑1 (MQ=255)
                                                            ggATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGtt                                                                                       <  1:1379705/127‑1 (MQ=255)
                                                            ggATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGtt                                                                                       >  2:1379705/1‑127 (MQ=255)
                                                               tAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCtata                                                                        >  2:1290853/1‑139 (MQ=255)
                                                                       aaCAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCttt                                                                                                   >  1:1637259/1‑104 (MQ=255)
                                                                       aaCAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCttt                                                                                                   <  2:1637259/104‑1 (MQ=255)
                                                                       aaCAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCg                                                                   >  2:115280/1‑136 (MQ=255)
                                                                       aaCAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCg                                                                   <  1:115280/136‑1 (MQ=255)
                                                                             ttATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAg                                                          <  2:1045555/139‑1 (MQ=255)
                                                                              tATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATAtct                                                                     >  2:78514/1‑127 (MQ=255)
                                                                              tATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATAtct                                                                     <  1:78514/127‑1 (MQ=255)
                                                                                ttGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATAc                                                                                                                            <  2:497677/70‑1 (MQ=255)
                                                                                ttGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATAc                                                                                                                            >  1:497677/1‑70 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACa                                                                                                                           <  2:1164200/65‑1 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACa                                                                                                                           >  1:1164200/1‑65 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGAt                                                                                                               <  1:489373/77‑1 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGAt                                                                                                               >  2:489373/1‑77 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAAcc                                                                                              >  1:1439416/1‑94 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAAcc                                                                                              <  1:1392373/94‑1 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAAcc                                                                                              >  2:1392373/1‑94 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAAcc                                                                                              <  2:1439416/94‑1 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGt                                                         <  1:1382237/131‑1 (MQ=255)
                                                                                      aCTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGt                                                         >  2:1382237/1‑131 (MQ=255)
                                                                                          ccTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCtt                                                                                                    <  2:346331/84‑1 (MQ=255)
                                                                                          ccTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCtt                                                                                                    >  1:346331/1‑84 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCttt                                                                                                   <  2:313982/77‑1 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCttt                                                                                                   >  1:313982/1‑77 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGActc                                                                                  <  2:418987/94‑1 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGActc                                                                                  <  2:993884/94‑1 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGActc                                                                                  >  1:418987/1‑94 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATAtct                                                                     >  2:834837/1‑107 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATAtct                                                                     <  1:834837/107‑1 (MQ=255)
                                                                                                  aaCATCTTAACTCAATGATTCTACGTGTTGATATGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGActc                                                                                  >  1:993884/1‑94 (MQ=255)
                                                                                                    cATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACa                                                                                                                           <  2:1596375/51‑1 (MQ=255)
                                                                                                    cATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACa                                                                                                                           >  1:1596375/1‑51 (MQ=255)
                                                                                                    cATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAaaagca                                             >  2:211514/1‑129 (MQ=255)
                                                                                                    cATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAaaagca                                             <  1:211514/129‑1 (MQ=255)
                                                                                                    cATCTTAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAAc                                   >  1:602501/1‑139 (MQ=255)
                                                                                                         tAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAACATGAt                              <  2:1095880/139‑1 (MQ=255)
                                                                                                         tAACTCAATGATTCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAACATGAt                              <  1:1290853/139‑1 (MQ=255)
                                                                                                                     tCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTg                                                                                <  1:652336/77‑1 (MQ=255)
                                                                                                                     tCTACGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTg                                                                                >  2:652336/1‑77 (MQ=255)
                                                                                                                         cGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAgg                                                                                          >  2:297224/1‑63 (MQ=255)
                                                                                                                         cGTGTTGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAgg                                                                                          <  1:297224/63‑1 (MQ=255)
                                                                                                                              tGATCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAACATGATAAGCATATTAAAACTCAACgg         <  1:953964/139‑1 (MQ=255)
                                                                                                                                aTCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTatat                                                                       <  1:1647358/75‑1 (MQ=255)
                                                                                                                                aTCTGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTatat                                                                       >  2:1647358/1‑75 (MQ=255)
                                                                                                                                   tGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGActc                                                                                  <  1:276814/61‑1 (MQ=255)
                                                                                                                                   tGCATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGActc                                                                                  >  2:276814/1‑61 (MQ=255)
                                                                                                                                     cATAACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAACATGATAAGCATATTAAAACTCAACGGAAAGACt  >  2:118218/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TTGGCCAGGATACTATATGACATACGATAGTGAATTCGGATCACATGTATCCCTATATCGGGATAGAATCAAACAGGTTATTGATGACTCCCTAAACGAACATCTTAACTCAATGATTCTACGTGTTGATCTGCATGACCCAATTGATACAGAAAATATGGATAACCCATTCTTTCAACCCAGGGTTGACTCTGGTGCTATATCTCGCTTTACCAGTGCGTTAAAAGCAAAGCTTAAACATGATAAGCATATTAAAACTCAACGGAAAGACT  >  NC_000913/2760531‑2760802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: