Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,913,654 T→C intergenic (‑3/+45) relA ← / ← rlmD (p)ppGpp synthetase I/GTP pyrophosphokinase/23S rRNA m(5)U1939 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,913,6540TC100.0% 243.8 / NA 69intergenic (‑3/+45)relA/rlmD(p)ppGpp synthetase I/GTP pyrophosphokinase/23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/34);  total (35/34)

CCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCT  >  NC_000913/2913535‑2913789
                                                                                                                       |                                                                                                                                       
ccAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAg                                                                                                                      >  1:224098/1‑139 (MQ=255)
          gCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGaa                                                                                                            >  2:118103/1‑139 (MQ=255)
              aGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCg                                                                                                        >  2:230511/1‑139 (MQ=255)
                    cacaCGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAt                                                                                                  <  1:230511/139‑1 (MQ=255)
                      cacGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCg                                                                                                <  1:557020/139‑1 (MQ=255)
                          aCTTCTGGCTGGTAATACCCAGACTTGCGATCCATTCTTCCGGATCAACTTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCaat                                                                                            >  1:660004/1‑138 (MQ=255)
                             tCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCct                                                                                                                                      >  2:385260/1‑94 (MQ=255)
                             tCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCct                                                                                                                                      <  1:385260/94‑1 (MQ=255)
                             tCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTAt                                                                                         >  2:960662/1‑139 (MQ=255)
                                 gctggTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTaa                                                                                     >  1:784534/1‑139 (MQ=255)
                                     gTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAt                                                                                                  >  1:1107214/1‑122 (MQ=255)
                                     gTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAt                                                                                                  <  2:1107214/122‑1 (MQ=255)
                                      tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCg                                                                                                <  2:1431522/123‑1 (MQ=255)
                                      tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCg                                                                                                >  1:1431522/1‑123 (MQ=255)
                                      tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgc                                                                                <  2:660004/139‑1 (MQ=255)
                                          aCCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCgaga                                                                            <  1:554857/139‑1 (MQ=255)
                                               gACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcg                                                                                 <  1:514789/129‑1 (MQ=255)
                                               gACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcg                                                                                 >  2:514789/1‑129 (MQ=255)
                                                    gCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                  >  1:1657045/1‑139 (MQ=255)
                                                     cGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATc                                                                 <  2:224098/139‑1 (MQ=255)
                                                          cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCgag                                                                             <  2:1105795/122‑1 (MQ=255)
                                                          cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCgag                                                                             >  1:1105795/1‑122 (MQ=255)
                                                             ttttCCGAATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAg                                                         <  2:866369/139‑1 (MQ=255)
                                                              tttCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGa                                                        <  2:1657045/139‑1 (MQ=255)
                                                                  cGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                    >  1:635961/1‑139 (MQ=255)
                                                                       cAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg                                               <  2:1066437/139‑1 (MQ=255)
                                                                                   ccTTATTGATATGTGCACTTCTTACCGCAACCATCGCCct                                                                                                                                      >  2:1163106/1‑40 (MQ=255)
                                                                                   ccTTATTGATATGTGCACTTCTTACCGCAACCATCGCCct                                                                                                                                      <  1:1163106/40‑1 (MQ=255)
                                                                                       aTTGATATGTGCACTTCTTACCGCGACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa                               >  1:1132038/1‑139 (MQ=255)
                                                                                             atGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCa                                                                                                     <  1:521445/63‑1 (MQ=255)
                                                                                             atGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCa                                                                                                     >  2:521445/1‑63 (MQ=255)
                                                                                             atGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                 >  2:836447/1‑115 (MQ=255)
                                                                                             atGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                 <  1:836447/115‑1 (MQ=255)
                                                                                              tgtgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGc                        <  2:635961/139‑1 (MQ=255)
                                                                                               gtgCACTTCTTACCGCAACCATCGCCCTCTCCttt                                                                                                                               >  1:1048269/1‑35 (MQ=255)
                                                                                               gtgCACTTCTTACCGCAACCATCGCCCTCTCCttt                                                                                                                               <  2:1048269/35‑1 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                  >  1:728307/1‑95 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                  >  2:1569859/1‑95 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                  <  1:1569859/95‑1 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                  <  2:728307/95‑1 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                    <  1:1589916/109‑1 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                    >  2:1589916/1‑109 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                           <  2:62702/118‑1 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                           >  1:62702/1‑118 (MQ=255)
                                                                                                tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGAGTGGGAACATATCCAGCATCGCCa                      >  1:1507979/1‑139 (MQ=255)
                                                                                                  cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTAcc                                                                    >  2:1404338/1‑91 (MQ=255)
                                                                                                  cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTAcc                                                                    <  1:1404338/91‑1 (MQ=255)
                                                                                                  cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCa                                                          <  1:902541/101‑1 (MQ=255)
                                                                                                  cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCa                                                          >  2:902541/1‑101 (MQ=255)
                                                                                                  cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                    >  2:518911/1‑139 (MQ=255)
                                                                                                       cttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAt                                                                                                  >  2:531081/1‑56 (MQ=255)
                                                                                                       cttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAt                                                                                                  <  1:531081/56‑1 (MQ=255)
                                                                                                       cttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                    >  2:1620947/1‑102 (MQ=255)
                                                                                                       cttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                    <  1:1620947/102‑1 (MQ=255)
                                                                                                        ttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa              <  1:118103/139‑1 (MQ=255)
                                                                                                          aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAt                                                                                                  >  1:1072316/1‑53 (MQ=255)
                                                                                                          aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAAt                                                                                                  <  2:1072316/53‑1 (MQ=255)
                                                                                                          aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                >  1:917834/1‑135 (MQ=255)
                                                                                                          aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                <  2:917834/135‑1 (MQ=255)
                                                                                                          aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa              <  2:452021/137‑1 (MQ=255)
                                                                                                          aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa              >  1:452021/1‑137 (MQ=255)
                                                                                                               aaCCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa              >  1:1423082/1‑132 (MQ=255)
                                                                                                               aaCCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa              <  2:1423082/132‑1 (MQ=255)
                                                                                                                 ccATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACatat                                  >  2:1309023/1‑110 (MQ=255)
                                                                                                                 ccATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACatat                                  <  1:1309023/110‑1 (MQ=255)
                                                                                                                 ccATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTatat     >  1:162408/1‑139 (MQ=255)
                                                                                                                    tCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                 >  1:1631940/1‑92 (MQ=255)
                                                                                                                    tCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                 <  2:1631940/92‑1 (MQ=255)
                                                                                                                    tCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCt  <  1:1604753/139‑1 (MQ=255)
                                                                                                                       |                                                                                                                                       
CCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCT  >  NC_000913/2913535‑2913789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: