Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,699	C→T	*434* (TAG→TAA)	rlmD ←	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,699	0	C	T	100.0%	223.1 / NA	69	*434* (TAG→TAA)	rlmD	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/37);  total (32/37)

TCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAG  >  NC_000913/2913564‑2913829                                                                                                                                        |                                                                                                                                   tCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTAt                                                                                                                                 >  2:960662/1‑139 (MQ=255)     gctggTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTaa                                                                                                                             >  1:784534/1‑139 (MQ=255)          tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgc                                                                                                                        <  2:660004/139‑1 (MQ=255)              aCCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCgaga                                                                                                                    <  1:554857/139‑1 (MQ=255)                   gACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcg                                                                                                                         >  2:514789/1‑129 (MQ=255)                   gACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcg                                                                                                                         <  1:514789/129‑1 (MQ=255)                        gCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                          >  1:1657045/1‑139 (MQ=255)                         cGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATc                                                                                                         <  2:224098/139‑1 (MQ=255)                              cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCgag                                                                                                                     >  1:1105795/1‑122 (MQ=255)                              cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCgag                                                                                                                     <  2:1105795/122‑1 (MQ=255)                                 ttttCCGAATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAg                                                                                                 <  2:866369/139‑1 (MQ=255)                                  tttCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGa                                                                                                <  2:1657045/139‑1 (MQ=255)                                      cGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                                                            >  1:635961/1‑139 (MQ=255)                                           cAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg                                                                                       <  2:1066437/139‑1 (MQ=255)                                                           aTTGATATGTGCACTTCTTACCGCGACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa                                                                       >  1:1132038/1‑139 (MQ=255)                                                                 atGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                         >  2:836447/1‑115 (MQ=255)                                                                 atGTGCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                         <  1:836447/115‑1 (MQ=255)                                                                  tgtgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGc                                                                <  2:635961/139‑1 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                          <  2:728307/95‑1 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                          >  1:728307/1‑95 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                          >  2:1569859/1‑95 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                          <  1:1569859/95‑1 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                                                            <  1:1589916/109‑1 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                                                            >  2:1589916/1‑109 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                                                                   <  2:62702/118‑1 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                                                                   >  1:62702/1‑118 (MQ=255)                                                                    tgCACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGAGTGGGAACATATCCAGCATCGCCa                                                              >  1:1507979/1‑139 (MQ=255)                                                                      cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTAcc                                                                                                            <  1:1404338/91‑1 (MQ=255)                                                                      cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTAcc                                                                                                            >  2:1404338/1‑91 (MQ=255)                                                                      cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCa                                                                                                  <  1:902541/101‑1 (MQ=255)                                                                      cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCa                                                                                                  >  2:902541/1‑101 (MQ=255)                                                                      cACTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                            >  2:518911/1‑139 (MQ=255)                                                                           cttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                                                            >  2:1620947/1‑102 (MQ=255)                                                                           cttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc                                                                                            <  1:1620947/102‑1 (MQ=255)                                                                            ttACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      <  1:118103/139‑1 (MQ=255)                                                                              aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                        <  2:917834/135‑1 (MQ=255)                                                                              aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                        >  1:917834/1‑135 (MQ=255)                                                                              aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      >  1:452021/1‑137 (MQ=255)                                                                              aCCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      <  2:452021/137‑1 (MQ=255)                                                                                   aaCCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      <  2:1423082/132‑1 (MQ=255)                                                                                   aaCCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      >  1:1423082/1‑132 (MQ=255)                                                                                     ccATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACatat                                                                          >  2:1309023/1‑110 (MQ=255)                                                                                     ccATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACatat                                                                          <  1:1309023/110‑1 (MQ=255)                                                                                     ccATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTatat                                             >  1:162408/1‑139 (MQ=255)                                                                                        tCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                         >  1:1631940/1‑92 (MQ=255)                                                                                        tCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                         <  2:1631940/92‑1 (MQ=255)                                                                                        tCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCt                                          <  1:1604753/139‑1 (MQ=255)                                                                                               ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGa                                                                                >  1:1583125/1‑94 (MQ=255)                                                                                               ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGa                                                                                <  2:1583125/94‑1 (MQ=255)                                                                                               ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc                                            >  1:879188/1‑130 (MQ=255)                                                                                               ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc                                            <  2:879188/130‑1 (MQ=255)                                                                                                tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                          <  1:1036026/67‑1 (MQ=255)                                                                                                tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                          >  2:1036026/1‑67 (MQ=255)                                                                                                     tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      >  1:892426/1‑114 (MQ=255)                                                                                                     tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      <  2:892426/114‑1 (MQ=255)                                                                                                            cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGaa                                                                                                                   >  1:1032330/1‑46 (MQ=255)                                                                                                            cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGaa                                                                                                                   <  2:1032330/46‑1 (MQ=255)                                                                                                            cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTggg                                                                                 >  2:30904/1‑80 (MQ=255)                                                                                                            cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTggg                                                                                 <  1:30904/80‑1 (MQ=255)                                                                                                            cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCt                      >  1:1426124/1‑139 (MQ=255)                                                                                                               aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                        >  2:1328995/1‑102 (MQ=255)                                                                                                               aCCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                        <  1:1328995/102‑1 (MQ=255)                                                                                                                   gCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      >  1:856141/1‑100 (MQ=255)                                                                                                                   gCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                      <  2:856141/100‑1 (MQ=255)                                                                                                                     cGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGc             <  2:1426124/139‑1 (MQ=255)                                                                                                                               aaaTCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCa   <  2:784534/139‑1 (MQ=255)                                                                                                                               aaaTCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCa   <  1:960662/139‑1 (MQ=255)                                                                                                                               aaaTCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCa   <  2:1507979/139‑1 (MQ=255)                                                                                                                                aaTCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCAGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAg  <  1:518911/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TCTGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAG  >  NC_000913/2913564‑2913829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑