Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,980,963	G→A	S407L (TCG→TTG)	araE ←	arabinose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,980,963	0	G	A	100.0%	107.0 / NA	34	S407L (TCG→TTG)	araE	arabinose transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

GACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATAC  >  NC_000913/2980827‑2981080                                                                                                                                         |                                                                                                                      gACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAac                                                                                                                     >  1:529051/1‑139 (MQ=255)   cATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAacac                                                                                                                   <  2:1197291/139‑1 (MQ=255)        ttGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGt                                                                                                              <  1:1538015/139‑1 (MQ=255)                                      cccACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAAt                                                                                <  2:529051/139‑1 (MQ=255)                                      cccACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAAt                                                                                >  1:40856/1‑139 (MQ=255)                                                             aGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAAtt                                                         >  1:729504/1‑139 (MQ=255)                                                                    agCCAGAACGTACTGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACAcc                                                                                                                  <  2:303709/75‑1 (MQ=255)                                                                    agCCAGAACGTACTGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACAcc                                                                                                                  >  1:303709/1‑75 (MQ=255)                                                                         gAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAgg                                             >  1:660789/1‑139 (MQ=255)                                                                          aaCGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCGGTTCGtggtgg                                                                                                     <  2:879192/82‑1 (MQ=255)                                                                          aaCGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGtggtgg                                                                                                     >  1:879192/1‑82 (MQ=255)                                                                            cGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATc                                          <  2:660789/139‑1 (MQ=255)                                                                               aCCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCcac                                       >  1:624108/1‑139 (MQ=255)                                                                               aCCGGCAGCGCCAAGGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCACGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCcac                                       >  2:1017018/1‑139 (MQ=255)                                                                                         ccAATGCTTTCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTggcg                             <  1:1017018/139‑1 (MQ=255)                                                                                                 aTCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCa                                                      <  1:1147375/106‑1 (MQ=255)                                                                                                 aTCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCa                                                      >  2:1147375/1‑106 (MQ=255)                                                                                                  tCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGca                                                 <  2:1500927/110‑1 (MQ=255)                                                                                                  tCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGca                                                 >  1:1500927/1‑110 (MQ=255)                                                                                                          tgtCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATaa            >  2:500281/1‑139 (MQ=255)                                                                                                           gtCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg                                                                >  1:144427/1‑86 (MQ=255)                                                                                                           gtCAGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg                                                                <  2:144427/86‑1 (MQ=255)                                                                                                              aGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg        <  1:500281/139‑1 (MQ=255)                                                                                                              aGGAAGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg        <  2:40856/139‑1 (MQ=255)                                                                                                                  aGGTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAAt    <  2:624108/139‑1 (MQ=255)                                                                                                                    gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATcg                                                                              >  2:226106/1‑63 (MQ=255)                                                                                                                    gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATcg                                                                              <  1:226106/63‑1 (MQ=255)                                                                                                                    gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg                                                                >  2:844658/1‑77 (MQ=255)                                                                                                                    gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCgg                                                                <  1:844658/77‑1 (MQ=255)                                                                                                                    gTCGCGCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATac  >  2:1457175/1‑139 (MQ=255)                                                                                                                      cgcgCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCaga                                                    <  2:1195513/87‑1 (MQ=255)                                                                                                                      cgcgCCGATAATCATATTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCaga                                                    >  1:1195513/1‑87 (MQ=255)                                                                                                                                     atTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg        <  1:685507/116‑1 (MQ=255)                                                                                                                                     atTCAACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCgg        >  2:685507/1‑116 (MQ=255)                                                                                                                                         |                                                                                                                      GACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACGTACCGGCAGCGCCAATGCTATCAAGCAGTGTCAGGAAGGTCGCGCCGATAATCATATTCGACACCCAGTTCGTGGTGGTCGAACAGGTAATACCGAAATCGCGGCATTTCAGCGGCTGAATTTCAGAGCACAGGATCCACACCACTGGCGCGGCGCTCATCGCATAACCGGCAATAC  >  NC_000913/2980827‑2981080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑