Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	291,324	C→T	C8Y (TGT→TAT)	insA ←	IS1 repressor TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	291,324	0	C	T	100.0%	51.8 / NA	18	C8Y (TGT→TAT)	insA	IS1 repressor TnpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

GTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATA  >  NC_000913/291276‑291452                                                 |                                                                                                                                 gTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGccc                                        >  1:335323/1‑139 (MQ=255)  tGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCg                                       <  1:1285391/139‑1 (MQ=255)     ttttGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGAt                                    <  1:592722/139‑1 (MQ=255)      tttGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATg                                   <  1:1517985/139‑1 (MQ=255)                cGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGACTTTTCAGCCCGGATGTTTCACGTAc                         <  1:1282181/139‑1 (MQ=255)                  caccacCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCt                       <  2:458617/139‑1 (MQ=255)                       ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGcc                                         <  2:845742/116‑1 (MQ=255)                       ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGcc                                         >  1:845742/1‑116 (MQ=255)                       ccccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTc                  <  1:1163550/139‑1 (MQ=255)                        cccGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCg                 >  1:1383168/1‑139 (MQ=255)                           gTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAat              >  2:1149024/1‑139 (MQ=255)                                tAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTg         <  2:326166/139‑1 (MQ=255)                                  gCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGc       >  2:1586890/1‑139 (MQ=255)                                      aaCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTg         <  1:1550918/133‑1 (MQ=255)                                      aaCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTg         >  2:1550918/1‑133 (MQ=255)                                      aaCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGAt   <  1:1586890/139‑1 (MQ=255)                                      aaCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGAt   >  1:125431/1‑139 (MQ=255)                                       aCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATa  <  2:1383168/139‑1 (MQ=255)                                                 |                                                                                                                                 GTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATA  >  NC_000913/291276‑291452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑