Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,411	C→T	*394* (TAG→TAA)	yqeF ←	short chain acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,411	0	C	T	100.0%	156.3 / NA	49	*394* (TAG→TAA)	yqeF	short chain acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/24);  total (25/24)

AGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAG  >  NC_000913/2984275‑2984538                                                                                                                                         |                                                                                                                                aGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTa                                                                                                                               >  2:552637/1‑139 (MQ=255) aGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTa                                                                                                                               >  1:98375/1‑139 (MQ=255)        tgtCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCa                                                                                                                        >  1:1465849/1‑139 (MQ=255)              tCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCa                                                                                                                  <  2:727980/139‑1 (MQ=255)                    atatGTCATGAGAATGGAAAAATGAGGGAGCCGAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                            >  1:238740/1‑139 (MQ=255)                    atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                            >  2:108423/1‑139 (MQ=255)                          cATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                      >  2:749066/1‑139 (MQ=255)                            tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCcac                                                                                                    >  1:1034089/1‑139 (MQ=255)                                aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACAccc                                                                                                >  2:656416/1‑139 (MQ=255)                                  tGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTg                                                                                              >  2:689042/1‑139 (MQ=255)                                  tGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTg                                                                                              >  2:1298841/1‑139 (MQ=255)                                     aaaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                           <  2:1034089/139‑1 (MQ=255)                                       aaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcccc                                                                                         <  2:98375/139‑1 (MQ=255)                                          tGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCa                                                                                                           >  2:179900/1‑118 (MQ=255)                                          tGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCa                                                                                                           <  1:179900/118‑1 (MQ=255)                                               gAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATa                                                                                 <  1:108423/139‑1 (MQ=255)                                                   ccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACaaa                                                                             >  1:1343734/1‑139 (MQ=255)                                                     aGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                           <  2:238740/139‑1 (MQ=255)                                                          atgtatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGc                                                                      <  2:1465849/139‑1 (MQ=255)                                                            gtatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                           <  2:1106940/116‑1 (MQ=255)                                                            gtatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                           >  1:1106940/1‑116 (MQ=255)                                                                 tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGt                                                                                                                          >  1:182516/1‑80 (MQ=255)                                                                 tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGt                                                                                                                          <  2:182516/80‑1 (MQ=255)                                                                 tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                             <  2:972123/93‑1 (MQ=255)                                                                 tCGAATTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCATAGTTATTCGTCACGTTCAATGGt                                                                                                             >  1:972123/1‑93 (MQ=255)                                                                   gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                             <  1:749066/139‑1 (MQ=255)                                                                    aCTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg                                                            <  1:656416/139‑1 (MQ=255)                                                                     cTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGc                                                           <  1:1298841/139‑1 (MQ=255)                                                                               atcatTATTCAACAGATTAATGACGGCAGATATTTTTCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg                                                 <  1:689042/139‑1 (MQ=255)                                                                                       tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                                                                    >  1:267566/1‑112 (MQ=255)                                                                                       tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                                                                    <  2:267566/112‑1 (MQ=255)                                                                                              aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                  <  1:1245391/139‑1 (MQ=255)                                                                                              aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                  >  2:1245391/1‑139 (MQ=255)                                                                                              aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                  >  1:1477018/1‑139 (MQ=255)                                                                                                tAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGaa                                <  1:552637/139‑1 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                     >  2:1544456/1‑62 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                     <  1:1544456/62‑1 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgccg                                                                                    >  2:851058/1‑79 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgccg                                                                                    <  1:851058/79‑1 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                    >  2:931374/1‑127 (MQ=255)                                                                                                        gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                    <  1:931374/127‑1 (MQ=255)                                                                                                                       tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCaa         >  1:1368471/1‑139 (MQ=255)                                                                                                                         aggaggAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAAcc       >  1:432943/1‑139 (MQ=255)                                                                                                                              gaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg  <  2:1477018/139‑1 (MQ=255)                                                                                                                                 aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                           >  1:1254184/1‑63 (MQ=255)                                                                                                                                 aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                           <  2:1254184/63‑1 (MQ=255)                                                                                                                                 aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                  >  1:1247190/1‑104 (MQ=255)                                                                                                                                 aTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                  <  2:1247190/104‑1 (MQ=255)                                                                                                                                      gagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                     <  1:1455479/80‑1 (MQ=255)                                                                                                                                         |                                                                                                                                AGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAG  >  NC_000913/2984275‑2984538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑