Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,997,689 C→T intergenic (‑89/+3) insC1 ← / ← ygeQ IS2 repressor TnpA/pseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,997,6890CT100.0% 294.1 / NA 93intergenic (‑89/+3)insC1/ygeQIS2 repressor TnpA/pseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/43);  total (50/43)

GTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAAT  >  NC_000913/2997557‑2997819
                                                                                                                                    |                                                                                                                                  
gtgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACt                                                                                                                              >  2:1627502/1‑139 (MQ=11)
 tgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACtt                                                                                                                             >  1:1045739/1‑139 (MQ=14)
           tCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttct                                                                                                                   >  2:1331385/1‑139 (MQ=255)
             tGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttcttc                                                                                                                 <  2:1045739/139‑1 (MQ=255)
                   tctcCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTg                                                                                                                            <  1:1498523/122‑1 (MQ=17)
                   tctcCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTg                                                                                                                            >  2:1498523/1‑122 (MQ=17)
                               aGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGaaa                                                                                               >  2:605578/1‑139 (MQ=255)
                                   atcaatcaTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGAtt                                                                                           >  2:1615272/1‑139 (MQ=255)
                                    tcaatcaTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGAttt                                                                                          >  1:1107721/1‑139 (MQ=255)
                                              gTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                <  1:1331385/139‑1 (MQ=255)
                                                      aTGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                >  1:23423/1‑131 (MQ=255)
                                                      aTGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                <  2:23423/131‑1 (MQ=255)
                                                      aTGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTa                                                                        >  1:566935/1‑139 (MQ=255)
                                                       tGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTaa                                                                       >  2:1420762/1‑139 (MQ=255)
                                                       tGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTaa                                                                       >  2:671290/1‑139 (MQ=255)
                                                        gACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAg                                                                      <  1:671290/139‑1 (MQ=255)
                                                        gACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAg                                                                      <  1:1420762/139‑1 (MQ=255)
                                                              tCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         >  2:1067041/1‑114 (MQ=255)
                                                              tCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         <  1:1067041/114‑1 (MQ=255)
                                                              tCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACa                                                                           <  1:1062895/128‑1 (MQ=255)
                                                              tCTAAAAACTAGTATTAAGACTATCACTTATTTAAGAGATATTGGTTTTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCATCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACa                                                                           >  2:1062895/1‑128 (MQ=255)
                                                                   aaaCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCAt                                                           <  2:566935/139‑1 (MQ=255)
                                                                        aGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGGGATTCAGGGGGCCAGTTTAAACTTGCTCTTttcttc                                                                                                                 >  2:930191/1‑80 (MQ=255)
                                                                        aGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGGGATTCAGGGGGCCAGTTTAAACTTGCTCTTttcttc                                                                                                                 <  1:930191/80‑1 (MQ=255)
                                                                               aGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGgtgt                                                                                                           >  2:467237/1‑79 (MQ=255)
                                                                               aGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGgtgt                                                                                                           <  1:467237/79‑1 (MQ=255)
                                                                               aGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         >  1:1407925/1‑97 (MQ=255)
                                                                               aGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         <  2:1407925/97‑1 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTg                                                                                                                            >  2:1276225/1‑57 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTg                                                                                                                            <  1:1276225/57‑1 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGa                                                                                                 >  1:102831/1‑84 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGa                                                                                                 <  2:102831/84‑1 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAg                                                                 >  2:352743/1‑116 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCa                                                            >  2:460843/1‑121 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCa                                                            <  1:460843/121‑1 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCgg                                                        <  2:133663/125‑1 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCgg                                                        >  1:133663/1‑125 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGt                                          >  2:143212/1‑139 (MQ=255)
                                                                                    aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTATTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAg                                                                 <  1:352743/116‑1 (MQ=255)
                                                                                     tCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTg                                                                                                               <  1:491808/69‑1 (MQ=255)
                                                                                     tCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTg                                                                                                               >  2:491808/1‑69 (MQ=255)
                                                                                      cACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCtt                                                     <  1:1390978/126‑1 (MQ=255)
                                                                                      cACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCtt                                                     >  2:1390978/1‑126 (MQ=255)
                                                                                               aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCtt                                                                                                                       >  1:620074/1‑51 (MQ=255)
                                                                                               aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCtt                                                                                                                       <  2:620074/51‑1 (MQ=255)
                                                                                               aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         >  2:19219/1‑81 (MQ=255)
                                                                                               aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         <  1:19219/81‑1 (MQ=255)
                                                                                                aGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTtct                                                   <  1:1127833/118‑1 (MQ=255)
                                                                                                aGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTtct                                                   >  2:1127833/1‑118 (MQ=255)
                                                                                                aGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTcc                              >  1:623290/1‑139 (MQ=255)
                                                                                                 gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttcttc                                                                                                                 <  2:127139/55‑1 (MQ=255)
                                                                                                 gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTttcttc                                                                                                                 >  1:127139/1‑55 (MQ=255)
                                                                                                 gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                <  2:698672/88‑1 (MQ=255)
                                                                                                 gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                >  1:698672/1‑88 (MQ=255)
                                                                                                   gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTa                                                                                                        >  2:512328/1‑62 (MQ=255)
                                                                                                   gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTa                                                                                                        <  1:512328/62‑1 (MQ=255)
                                                                                                   gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGa                                                                                                 >  2:204301/1‑69 (MQ=255)
                                                                                                   gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGa                                                                                                 <  1:204301/69‑1 (MQ=255)
                                                                                                    atatTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGAt                          <  1:143212/139‑1 (MQ=255)
                                                                                                     tatTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTtct                                                   >  1:1133258/1‑113 (MQ=255)
                                                                                                     tatTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTtct                                                   <  2:1133258/113‑1 (MQ=255)
                                                                                                      atTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTa                                                                                       >  2:641166/1‑76 (MQ=255)
                                                                                                      atTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTa                                                                                       <  1:641166/76‑1 (MQ=255)
                                                                                                      atTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATaa                        >  2:1460139/1‑139 (MQ=255)
                                                                                                      atTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCACTTttcttc                                                                                                                 >  2:1201469/1‑50 (MQ=17)
                                                                                                      atTGGTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCACTTttcttc                                                                                                                 <  1:1201469/50‑1 (MQ=17)
                                                                                                       ttGGTTGTCTGGAGATTCAAGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAAc                       >  2:1113509/1‑139 (MQ=255)
                                                                                                          gTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTg                                                                                                                            <  1:616152/35‑1 (MQ=14)
                                                                                                          gTTGTCTGGAGATTCAGGGGGCCAGTTTAAACTTg                                                                                                                            >  2:616152/1‑35 (MQ=255)
                                                                                                           ttGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTgg                   <  1:1627502/139‑1 (MQ=255)
                                                                                                            tGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                >  2:1554987/1‑77 (MQ=255)
                                                                                                            tGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                <  1:1554987/77‑1 (MQ=255)
                                                                                                            tGTCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTggg                  >  2:986919/1‑139 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCttt                                                                                                   <  2:296490/56‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCttt                                                                                                   >  1:296490/1‑56 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         >  1:746604/1‑66 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTg                                                                                         <  2:746604/66‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg                                                                                     <  2:1657536/70‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg                                                                                     >  2:51510/1‑70 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg                                                                                     >  1:1657536/1‑70 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAgg                                                                                     <  1:51510/70‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                >  1:1145341/1‑75 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGataaa                                                                                <  2:1145341/75‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAg                                                                      >  2:771030/1‑85 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAg                                                                      <  1:771030/85‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGa                           <  2:625742/128‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGa                           <  2:629980/128‑1 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGa                           >  1:625742/1‑128 (MQ=255)
                                                                                                              tCTGGAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGa                           >  1:629980/1‑128 (MQ=255)
                                                                                                                 ggAGATTCAGGGGGCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATtc             <  1:1113509/139‑1 (MQ=255)
                                                                                                                            gggCCAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAAt  >  2:1376265/1‑139 (MQ=255)
                                                                                                                                cAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAg                                                                                              >  1:917956/1‑43 (MQ=255)
                                                                                                                                cAGTTTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAg                                                                                              <  2:917956/43‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
GTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTGTTTATCTTTGAAAGATTTGTAGGATAAATAACATTAAGTAAAGCCGCATCGGCTTCTCTGTAATGTTTATCTTTTTCCAGATAACCTGGGGATTCTCCCCCTAAAT  >  NC_000913/2997557‑2997819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: