Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,056,273 G→A P11P (CCG→CCA)  fau → 5‑formyltetrahydrofolate cyclo‑ligase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,056,2730GA100.0% 267.5 / NA 83P11P (CCG→CCA) fau5‑formyltetrahydrofolate cyclo‑ligase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/41);  total (42/41)

ACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACT  >  NC_000913/3056138‑3056407
                                                                                                                                       |                                                                                                                                      
aCAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaa                                                                                                                                     >  2:820755/1‑139 (MQ=255)
aCAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaa                                                                                                                                     >  1:327572/1‑139 (MQ=255)
aCAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaa                                                                                                                                     >  2:1305012/1‑139 (MQ=255)
            ggcATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaac                                                                                                                                 <  1:832859/131‑1 (MQ=255)
            ggcATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaac                                                                                                                                 >  2:832859/1‑131 (MQ=255)
              cATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAAt                                                                                                                       <  1:820755/139‑1 (MQ=255)
                          tCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                               >  1:92081/1‑119 (MQ=255)
                          tCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcag                                                                                                                               <  2:92081/119‑1 (MQ=255)
                           cccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGcc                                                                                                          >  1:1100436/1‑139 (MQ=255)
                            ccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCg                                                                                                         <  2:760573/139‑1 (MQ=255)
                                   aTCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                               <  1:1003778/126‑1 (MQ=255)
                                   aTCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                               >  2:1003778/1‑126 (MQ=255)
                                   aTCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCg                                                                                                  >  1:841621/1‑139 (MQ=255)
                                   aTCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGG‑AATGGGTCAACAAGCCGCTACCCgg                                                                                                 >  2:731412/1‑139 (MQ=255)
                                      tGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                               <  1:1179831/123‑1 (MQ=255)
                                      tGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaac                                                                                                               >  2:1179831/1‑123 (MQ=255)
                                       ggCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGG‑AATGGGTCAACAAGCCGCTACCCGgatga                                                                                             <  1:731412/139‑1 (MQ=255)
                                       ggCACCAGCCATGACGCAACCACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatg                                                                                              >  2:28300/1‑139 (MQ=255)
                                        gCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                           >  2:1386664/1‑125 (MQ=255)
                                        gCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                           <  1:1386664/125‑1 (MQ=255)
                                          aCCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGatgatg                                                                                           >  2:467055/1‑139 (MQ=255)
                                             aGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGa                                                                                                                          <  2:1667175/105‑1 (MQ=255)
                                             aGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGa                                                                                                                          >  1:1667175/1‑105 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaaca                                                                                                                                   <  2:793068/95‑1 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaaca                                                                                                                                   >  1:793068/1‑95 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                                    >  2:178248/1‑94 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                                    <  1:178248/94‑1 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatg                                                                                              >  1:1187393/1‑132 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatg                                                                                              <  2:1187393/132‑1 (MQ=255)
                                              gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACtt                                                                                       >  2:1381064/1‑139 (MQ=255)
                                                  tGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATcc                                                                                   >  1:1441693/1‑139 (MQ=255)
                                                   gACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATccc                                                                                  <  1:467055/139‑1 (MQ=255)
                                                   gACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATccc                                                                                  <  1:1305012/139‑1 (MQ=255)
                                                      gCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCg                                                                               <  1:1381064/139‑1 (MQ=255)
                                                      gCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCg                                                                               <  2:1111685/139‑1 (MQ=255)
                                                      gCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCg                                                                               <  2:841621/139‑1 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                                  <  2:640487/85‑1 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                                  >  1:640487/1‑85 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                           >  2:101659/1‑108 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGc                                                                                                           <  1:101659/108‑1 (MQ=255)
                                                         aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtg                                                                            >  1:1604020/1‑139 (MQ=255)
                                                            aCCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                         <  2:327572/139‑1 (MQ=255)
                                                            aCCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                         <  2:1100436/139‑1 (MQ=255)
                                                             ccAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAAAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggtca                                                                        >  1:986638/1‑137 (MQ=255)
                                                                    tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                                  <  1:1147214/74‑1 (MQ=255)
                                                                    tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                                  >  2:1147214/1‑74 (MQ=255)
                                                                    tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                         <  2:482936/131‑1 (MQ=255)
                                                                    tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                         >  1:482936/1‑131 (MQ=255)
                                                                        aCTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACg                                                             >  1:1187761/1‑139 (MQ=255)
                                                                          tGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGt                                                           <  2:1441693/139‑1 (MQ=255)
                                                                            aCATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                                         <  2:1187761/139‑1 (MQ=255)
                                                                                tATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                                    <  2:330959/60‑1 (MQ=255)
                                                                                tATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                                    >  1:330959/1‑60 (MQ=255)
                                                                                tATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGt                                                     >  2:494620/1‑139 (MQ=255)
                                                                                 aTCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTa                                                    <  1:1234725/139‑1 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAGCAACAGCAGGAAATgg                                                                                                                     <  2:1112202/68‑1 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAGCAACAGCAGGAAATgg                                                                                                                     >  1:1112202/1‑68 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCg                                                                                                         >  2:1214533/1‑80 (MQ=255)
                                                                                       aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCg                                                                                                         <  1:1214533/80‑1 (MQ=255)
                                                                                        cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                                    <  1:910339/52‑1 (MQ=255)
                                                                                        cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                                    >  2:910339/1‑52 (MQ=255)
                                                                                        cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGatgatg                                                                                           <  2:131047/93‑1 (MQ=255)
                                                                                        cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGatgatg                                                                                           >  1:131047/1‑93 (MQ=255)
                                                                                           gAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCtt                                          <  1:28300/139‑1 (MQ=255)
                                                                                           gAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCtt                                          >  1:219380/1‑139 (MQ=255)
                                                                                                   cAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGt                                                           >  2:1476101/1‑114 (MQ=255)
                                                                                                   cAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGt                                                           <  1:1476101/114‑1 (MQ=255)
                                                                                                           ttCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaaa                                                                                                                        <  1:1619244/45‑1 (MQ=255)
                                                                                                           ttCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaaa                                                                                                                        >  2:1619244/1‑45 (MQ=255)
                                                                                                             cGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGaca                        <  1:829961/139‑1 (MQ=255)
                                                                                                                cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACAccc                     >  2:716720/1‑139 (MQ=255)
                                                                                                                    gacgtcatGCCTTCACCCCAGAACCCCAGCGGGAAATTGGTCAACAAGCCGATACCAGCATGATGGCTTATCCCCCGGTGGTGATGGCAGATACCGTCGCAGTATTCCTCTCTTCTGCTGGCAAACTCGACACCCAGcc                 <  1:1338219/137‑1 (MQ=255)
                                                                                                                       gtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACt              <  2:1604020/139‑1 (MQ=255)
                                                                                                                       gtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACt              <  2:986638/139‑1 (MQ=255)
                                                                                                                         cgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTa                                                    <  1:1435772/99‑1 (MQ=255)
                                                                                                                         cgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGAATTATCACCCGGTGGTGATGGCACATACGGTCGCTGTa                                                    >  2:1435772/1‑99 (MQ=255)
                                                                                                                             cGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCgg                                                                              >  2:528095/1‑69 (MQ=255)
                                                                                                                             cGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCgg                                                                              <  1:528095/69‑1 (MQ=255)
                                                                                                                             cGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                                         >  2:184025/1‑90 (MQ=255)
                                                                                                                             cGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                                         <  1:184025/90‑1 (MQ=255)
                                                                                                                             cGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGa        >  2:954881/1‑139 (MQ=255)
                                                                                                                               ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgat                                                                                               <  1:1250410/50‑1 (MQ=255)
                                                                                                                               ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgat                                                                                               >  2:1250410/1‑50 (MQ=255)
                                                                                                                                   cGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAAct  >  1:812356/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACT  >  NC_000913/3056138‑3056407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑