Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,218,365	C→T	E238K (GAG→AAG)	aer ←	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,218,365	0	C	T	100.0%	166.4 / NA	51	E238K (GAG→AAG)	aer	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/26);  total (25/26)

GGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCA  >  NC_000913/3218243‑3218481                                                                                                                           |                                                                                                                     ggTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGa                                                                                                      <  1:498578/139‑1 (MQ=255)     ccTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                  <  1:1407888/139‑1 (MQ=255)         tCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAac                                                                                              >  2:1495202/1‑139 (MQ=255)                cGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTa                                                                                       >  1:1156268/1‑139 (MQ=255)                          ccATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGa                                                                                                            >  2:20554/1‑108 (MQ=255)                          ccATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGa                                                                                                            <  1:20554/108‑1 (MQ=255)                            aTTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATg                                                                                                    >  2:1432356/1‑114 (MQ=255)                            aTTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATg                                                                                                    <  1:1432356/114‑1 (MQ=255)                                      cTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCa                                                                 >  2:703030/1‑139 (MQ=255)                                         gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                  <  2:340516/103‑1 (MQ=255)                                         gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                  >  1:340516/1‑103 (MQ=255)                                         gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCgg                                                                             <  2:468744/124‑1 (MQ=255)                                         gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCgg                                                                             >  1:468744/1‑124 (MQ=255)                                                   ttGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGc                                                    <  1:1495202/139‑1 (MQ=255)                                                     gagaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAg                                                                                                       >  1:1581515/1‑86 (MQ=255)                                                     gagaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAg                                                                                                       <  2:1581515/86‑1 (MQ=255)                                                     gagaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                  <  2:415615/91‑1 (MQ=255)                                                     gagaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                  >  1:415615/1‑91 (MQ=255)                                                      agaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGa                                                                                                            <  2:448394/80‑1 (MQ=255)                                                      agaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGa                                                                                                            >  1:448394/1‑80 (MQ=255)                                                      agaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAAc                                                 >  1:1426400/1‑139 (MQ=255)                                                           tCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATggg                                                     >  1:1294951/1‑130 (MQ=255)                                                           tCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATggg                                                     <  2:1294951/130‑1 (MQ=255)                                                                    aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                  <  1:1458245/76‑1 (MQ=255)                                                                    aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                  >  2:1458245/1‑76 (MQ=255)                                                                    aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCaa                                                                                               <  2:1087796/79‑1 (MQ=255)                                                                    aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCaa                                                                                               >  1:1087796/1‑79 (MQ=255)                                                                     gCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcg                                  <  1:703030/139‑1 (MQ=255)                                                                          cGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCgg                                                                             >  2:439495/1‑91 (MQ=255)                                                                          cGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCgg                                                                             <  1:439495/91‑1 (MQ=255)                                                                               caTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGc                        >  1:1233035/1‑139 (MQ=255)                                                                                   aGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCAtt                    <  2:1109930/139‑1 (MQ=255)                                                                                    ggCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTATGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTc                   >  2:771769/1‑139 (MQ=255)                                                                                     gCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACTTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGc                                                    >  1:955890/1‑105 (MQ=255)                                                                                     gCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGc                                                    <  2:955890/105‑1 (MQ=255)                                                                                            tGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTATGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAgg           <  1:771769/139‑1 (MQ=255)                                                                                            tGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAgg           <  2:1233035/139‑1 (MQ=255)                                                                                              cccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGgcg         <  2:1156268/139‑1 (MQ=255)                                                                                               ccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGc                                                             <  2:211657/86‑1 (MQ=255)                                                                                               ccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGc                                                             <  2:441567/86‑1 (MQ=255)                                                                                               ccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGc                                                             >  1:441567/1‑86 (MQ=255)                                                                                               ccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGc                                                             >  1:211657/1‑86 (MQ=255)                                                                                                   ccGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGc    >  2:801767/1‑139 (MQ=255)                                                                                                        cGTAATGTCAGCCCC‑GCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                   <  2:1008377/86‑1 (MQ=255)                                                                                                        cGTAATGTCAGCCCC‑GCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                   >  1:1008377/1‑86 (MQ=255)                                                                                                                 aGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc                                                                           >  1:1445975/1‑54 (MQ=255)                                                                                                                 aGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc                                                                           <  2:1445975/54‑1 (MQ=255)                                                                                                                 aGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCca  <  1:744465/127‑1 (MQ=255)                                                                                                                 aGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCca  >  2:744465/1‑127 (MQ=255)                                                                                                                   ccgcAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTg     <  2:412759/119‑1 (MQ=255)                                                                                                                   ccgcAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTg     >  1:412759/4‑122 (MQ=255)                                                                                                                           |                                                                                                                     GGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCA  >  NC_000913/3218243‑3218481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑