Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,012	A→G	S200G (AGT→GGT)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,012	0	A	G	100.0%	245.4 / NA	67	S200G (AGT→GGT)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/32);  total (35/32)

TGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGA  >  NC_000913/3268881‑3269142                                                                                                                                    |                                                                                                                                   tGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAac                                                                                                                             >  2:1513893/1‑139 (MQ=255)  ggACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAaca                                                                                                                            >  2:1516301/1‑139 (MQ=255)         cGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACg                                                                                                                     >  2:779739/1‑139 (MQ=255)              tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTCAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                <  1:971649/139‑1 (MQ=255)                                           aTAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAA‑TTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                >  1:1001040/1‑109 (MQ=255)                                           aTAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAA‑TTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                <  2:1001040/109‑1 (MQ=255)                                             aaaCATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                 <  2:57392/139‑1 (MQ=255)                                                     ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                <  1:1039710/100‑1 (MQ=255)                                                     ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTta                                                                         >  2:780539/1‑139 (MQ=255)                                                     ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTta                                                                         >  1:517251/1‑139 (MQ=255)                                                     ggCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGGTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                >  2:1039710/1‑100 (MQ=255)                                                       cTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAt                                                                                                              <  2:65107/100‑1 (MQ=255)                                                       cTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAt                                                                                                              >  1:65107/1‑100 (MQ=255)                                                       cTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                       >  2:259954/1‑139 (MQ=255)                                                        tAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaata                                                                      <  1:780539/139‑1 (MQ=255)                                                          aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                          >  1:1377882/1‑101 (MQ=255)                                                          aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTcc                                                                                                          <  2:1377882/101‑1 (MQ=255)                                                          aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                 <  1:344646/126‑1 (MQ=255)                                                          aTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                 >  2:344646/1‑126 (MQ=255)                                                           tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                             <  1:1111476/129‑1 (MQ=255)                                                           tACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                             >  2:1111476/1‑129 (MQ=255)                                                            aCTCTTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                  <  2:1076688/139‑1 (MQ=255)                                                                ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCca                                                                                                                  >  2:1552635/1‑87 (MQ=255)                                                                ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCca                                                                                                                  <  1:1552635/87‑1 (MQ=255)                                                                ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAg                                                              >  2:278542/1‑139 (MQ=255)                                                                ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAg                                                              >  2:150227/1‑139 (MQ=255)                                                                 tttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                             <  1:1516301/139‑1 (MQ=255)                                                                 tttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                             <  1:278542/139‑1 (MQ=255)                                                                     aaTCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGata                                                         >  1:1532410/1‑139 (MQ=255)                                                                          ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                >  2:947996/1‑79 (MQ=255)                                                                          ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                <  1:947996/79‑1 (MQ=255)                                                                          ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTg                                                                              <  2:304498/113‑1 (MQ=255)                                                                          ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTg                                                                              >  1:304498/1‑113 (MQ=255)                                                                          ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGtt                                                                            <  1:180333/115‑1 (MQ=255)                                                                          ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGtt                                                                            >  2:180333/1‑115 (MQ=255)                                                                                  tGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                <  1:1404372/71‑1 (MQ=255)                                                                                  tGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                >  2:1404372/1‑71 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                <  1:858507/69‑1 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                >  2:858507/1‑69 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTa                                                                                           >  2:1479301/1‑90 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTa                                                                                           <  1:1479301/90‑1 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACga                                                                                 >  2:748835/1‑99 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                 <  1:748835/100‑1 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                  <  1:359524/115‑1 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCa                                                                  >  2:359524/1‑115 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGaaa                                          >  1:549461/1‑139 (MQ=255)                                                                                    aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGaaa                                          >  2:856749/1‑139 (MQ=255)                                                                                         aacaaATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGtt                                                                            <  2:1431321/100‑1 (MQ=255)                                                                                         aacaaATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGtt                                                                            >  1:1431321/1‑100 (MQ=255)                                                                                                       aTGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                       >  2:796662/1‑139 (MQ=255)                                                                                                         gCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgtt                     >  1:1061972/1‑139 (MQ=255)                                                                                                          ccAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAtctc                                                                                                           <  2:366408/52‑1 (MQ=255)                                                                                                          ccAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAAtctc                                                                                                           >  1:366408/1‑52 (MQ=255)                                                                                                          ccAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgttg                    >  1:295834/1‑139 (MQ=255)                                                                                                                tGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa              <  2:1532410/139‑1 (MQ=255)                                                                                                                  tttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGatat                                                        <  1:1070345/95‑1 (MQ=255)                                                                                                                  tttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGatat                                                        >  2:1070345/1‑95 (MQ=255)                                                                                                                  tttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATg            <  1:779739/139‑1 (MQ=255)                                                                                                                   ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                 <  1:1632126/69‑1 (MQ=255)                                                                                                                   ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                 >  2:1632126/1‑69 (MQ=255)                                                                                                                   ttCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGa           <  2:295834/139‑1 (MQ=255)                                                                                                                    tCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                <  1:20026/37‑1 (MQ=255)                                                                                                                    tCAGGTTAATTTTGTTGGTTCAACACAAAACGCcaca                                                                                                                >  2:20026/1‑37 (MQ=255)                                                                                                                       ggTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCt       <  2:1061972/139‑1 (MQ=255)                                                                                                                         ttAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTgg     <  2:445461/139‑1 (MQ=255)                                                                                                                           aaTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGgag   >  1:608818/1‑139 (MQ=255)                                                                                                                            aTTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGgaga  <  2:549461/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   TGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGA  >  NC_000913/3268881‑3269142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑