Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,277,726 C→T F130F (TTC→TTT yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,7260CT100.0% 249.8 / NA 76F130F (TTC→TTTyhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/36);  total (40/36)

GGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACT  >  NC_000913/3277591‑3277860
                                                                                                                                       |                                                                                                                                      
ggTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGc                                                                                                                                     >  2:576904/1‑139 (MQ=255)
ggTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGc                                                                                                                                     >  2:471866/1‑139 (MQ=255)
ggTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGc                                                                                                                                     >  2:166697/1‑139 (MQ=255)
   aaaaTTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                                  <  1:166697/139‑1 (MQ=255)
                    gTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACa                                                                                                                 <  1:471866/139‑1 (MQ=255)
                           tctcTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATcctcct                                                                                                          >  1:1027662/1‑139 (MQ=255)
                                tcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGa                                                                                                     <  1:186031/139‑1 (MQ=255)
                                     cGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                                                                                                <  2:1027662/139‑1 (MQ=255)
                                         ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAc                                                                                            >  1:1145891/1‑139 (MQ=255)
                                           aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGAc                                                                                                                  >  2:1505118/1‑115 (MQ=255)
                                           aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGAc                                                                                                                  <  1:1505118/115‑1 (MQ=255)
                                           aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                                                                                          >  1:1339424/1‑139 (MQ=255)
                                           aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                                                                                          >  1:894541/1‑139 (MQ=255)
                                            gTGAAAAAGAGAAAGTCATCATTCAGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                                                                                         >  1:1132537/1‑139 (MQ=255)
                                             tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                                                                                        <  2:894541/139‑1 (MQ=255)
                                               aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAc                                                                                      <  1:273072/139‑1 (MQ=255)
                                                       aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTaaa                                                                                                                          <  1:514671/95‑1 (MQ=255)
                                                       aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTaaa                                                                                                                          >  2:514671/1‑95 (MQ=255)
                                                       aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa                                                                              >  1:943562/1‑139 (MQ=255)
                                                               catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgaga                                                                                <  1:93007/129‑1 (MQ=255)
                                                               catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgaga                                                                                >  2:93007/1‑129 (MQ=255)
                                                                  tCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAcc                                                                   <  2:1339424/139‑1 (MQ=255)
                                                                   cTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAccc                                                                  >  1:1365500/1‑139 (MQ=255)
                                                                         tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                            <  2:1365500/139‑1 (MQ=255)
                                                                          ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGACACAGAAGAAACCCATTGATg                                                           <  2:1132537/139‑1 (MQ=255)
                                                                           gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                    <  2:724520/65‑1 (MQ=255)
                                                                           gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCa                                                                                                                                    >  1:724520/1‑65 (MQ=255)
                                                                           gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga                                                                       >  1:1109052/1‑126 (MQ=255)
                                                                           gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga                                                                       <  2:1109052/126‑1 (MQ=255)
                                                                            atgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                                                   >  1:502086/1‑113 (MQ=255)
                                                                            atgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                                                   <  2:502086/113‑1 (MQ=255)
                                                                             tgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg                                                        >  1:677669/1‑139 (MQ=255)
                                                                                aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                                  <  2:618444/62‑1 (MQ=255)
                                                                                aCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCaaa                                                                                                                                  >  1:618444/1‑62 (MQ=255)
                                                                                     gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGa                                                                                                     <  2:1282831/86‑1 (MQ=255)
                                                                                     gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGa                                                                                                     >  1:1282831/1‑86 (MQ=255)
                                                                                             tctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                                                                                         <  1:816183/90‑1 (MQ=255)
                                                                                             tctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                                                                                         >  2:816183/1‑90 (MQ=255)
                                                                                             tctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAACAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgccg                                        >  1:1184858/1‑139 (MQ=255)
                                                                                              ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                                                           <  2:1490981/119‑1 (MQ=255)
                                                                                              ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                                                           >  1:1490981/1‑119 (MQ=255)
                                                                                              ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa                                       >  1:1590449/1‑139 (MQ=255)
                                                                                               tGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga                                                                       <  2:38106/106‑1 (MQ=255)
                                                                                               tGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCAGACTGGGAAACCCTCACCCGAGAAACagaaga                                                                       >  1:38106/1‑106 (MQ=255)
                                                                                                gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGa                                                                                               >  1:609487/1‑81 (MQ=255)
                                                                                                gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGa                                                                                               <  2:609487/81‑1 (MQ=255)
                                                                                                gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc                                     >  1:1414700/1‑139 (MQ=255)
                                                                                                gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc                                     >  1:562639/1‑139 (MQ=255)
                                                                                                    aCCTACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                                 <  2:562639/139‑1 (MQ=255)
                                                                                                       tACGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCg                              >  2:702658/1‑139 (MQ=255)
                                                                                                         cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAagag                                                                                                                     <  1:1043694/50‑1 (MQ=255)
                                                                                                         cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAagag                                                                                                                     >  2:1043694/1‑50 (MQ=255)
                                                                                                         cGGTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCgtg                            >  1:868507/1‑139 (MQ=255)
                                                                                                           gTAAAAAAACAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGtt                          <  2:943562/139‑1 (MQ=255)
                                                                                                                aaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                                                                                         <  1:224415/71‑1 (MQ=255)
                                                                                                                aaaaCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                                                                                         >  2:224415/1‑71 (MQ=255)
                                                                                                                   aCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                                                   >  2:666981/1‑74 (MQ=255)
                                                                                                                   aCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                                                                                   <  1:666981/74‑1 (MQ=255)
                                                                                                                   aCAGATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCg                  <  2:677669/139‑1 (MQ=255)
                                                                                                                     aGATGCCTATACCGTATTTAGCAAAGTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCCCCGAACGCATTCGTGTTGACCAGCggg                <  2:1079878/139‑1 (MQ=255)
                                                                                                                      gATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                            >  2:1575863/1‑94 (MQ=255)
                                                                                                                      gATGCCTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                            <  1:1575863/94‑1 (MQ=255)
                                                                                                                          ccTATACCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATGGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATcc           <  1:702658/139‑1 (MQ=255)
                                                                                                                              taCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCg                  >  2:106017/1‑128 (MQ=255)
                                                                                                                              taCCGTATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCg                  <  1:106017/128‑1 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGa                                                                                               >  1:811725/1‑46 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGa                                                                                               <  2:811725/46‑1 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                     >  2:1050347/1‑72 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa                                                                     <  1:1050347/72‑1 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                                                           >  2:36088/1‑82 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg                                                           <  1:36088/82‑1 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtctc     >  2:1594770/1‑136 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtctc     <  1:1594770/136‑1 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCAc   <  1:1278917/138‑1 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCAc   >  2:1278917/1‑138 (MQ=255)
                                                                                                                                   tATTTAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACt  >  2:443688/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACT  >  NC_000913/3277591‑3277860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: