Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,398,875 C→T M1M (GTG→ATG) †
*368* (TAG→TAA
mreD ←
mreC ←
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,398,8750CT100.0% 194.8 / NA 61M1M (GTG→ATG) †
*368* (TAG→TAA
mreD
mreC
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/25);  total (36/25)

CAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGC  >  NC_000913/3398747‑3399010
                                                                                                                                |                                                                                                                                       
caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTc                                                                                                                               >  2:159770/1‑139 (MQ=255)
           acacCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc                                                                                                                                  >  1:673700/1‑125 (MQ=255)
           acacCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc                                                                                                                                  <  2:673700/125‑1 (MQ=255)
              cccAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCa                                                                                                                 >  2:1187277/1‑139 (MQ=255)
                   tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                            >  2:455937/1‑139 (MQ=255)
                      ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTg                                                                                                         >  2:874571/1‑139 (MQ=255)
                           gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                            >  1:1346575/1‑131 (MQ=255)
                           gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                            <  2:1346575/131‑1 (MQ=255)
                           gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGcac                                                                                                    <  1:159770/139‑1 (MQ=255)
                             aaaCAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGcacca                                                                                                  >  1:1162093/1‑139 (MQ=255)
                                     aGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg                                                                                          <  1:1187277/139‑1 (MQ=255)
                                     aGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg                                                                                          <  1:874571/139‑1 (MQ=255)
                                      ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggc                                                                                         >  2:1624511/1‑139 (MQ=255)
                                      ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggc                                                                                         >  2:1057315/1‑139 (MQ=255)
                                           tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGc                                                                                                              >  2:928985/1‑113 (MQ=255)
                                           tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGc                                                                                                              <  1:928985/113‑1 (MQ=255)
                                            ccGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                                   <  2:1162093/139‑1 (MQ=255)
                                                    ggCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCttt                                                                            >  1:463788/1‑138 (MQ=255)
                                                    ggCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCttt                                                                            <  2:463788/138‑1 (MQ=255)
                                                    ggCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTtg                                                                           >  2:114165/1‑139 (MQ=255)
                                                        tGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg                                                                                     <  1:58350/125‑1 (MQ=255)
                                                        tGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg                                                                                     >  2:58350/1‑125 (MQ=255)
                                                                agcaACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAtt                                                               <  1:1624511/139‑1 (MQ=255)
                                                                     cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag                                                          >  2:968763/1‑139 (MQ=255)
                                                                     cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag                                                          <  1:114165/139‑1 (MQ=255)
                                                                        cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTAt                                                                                                                                      <  2:1221320/60‑1 (MQ=255)
                                                                        cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTAt                                                                                                                                      >  1:1221320/1‑60 (MQ=255)
                                                                                  aaaGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag                                                          >  2:442908/1‑126 (MQ=255)
                                                                                  aaaGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag                                                          <  1:442908/126‑1 (MQ=255)
                                                                                      agagCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgca                                         <  1:1057315/139‑1 (MQ=255)
                                                                                        agCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCgg                                             >  2:624170/1‑133 (MQ=255)
                                                                                        agCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCgg                                             <  1:624170/133‑1 (MQ=255)
                                                                                         gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt                                                                <  1:518786/113‑1 (MQ=255)
                                                                                         gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt                                                                >  2:518786/1‑113 (MQ=255)
                                                                                         gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagca                                      >  2:1213223/1‑139 (MQ=255)
                                                                                         gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagca                                      >  1:539263/1‑139 (MQ=255)
                                                                                         gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagca                                      >  1:663750/1‑139 (MQ=255)
                                                                                           cAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg                                                                                          >  1:1188413/1‑85 (MQ=255)
                                                                                           cAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg                                                                                          <  2:1188413/85‑1 (MQ=255)
                                                                                                  cccAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTa                             >  1:234935/1‑139 (MQ=255)
                                                                                                   ccAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCTGTAg                            <  1:968763/139‑1 (MQ=255)
                                                                                                       cGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc                        >  2:245342/1‑139 (MQ=255)
                                                                                                       cGTCCCTGGCTACGATAGCGCGCCATTATTGCCCTCCCCGCACCCGAGCAGGCGGTTGAGCACCACTCTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGCGCCGGCGCAGAAGGCGCacaagaagc                        >  1:176574/1‑131 (MQ=255)
                                                                                                        gTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt                                                                >  2:1434394/1‑98 (MQ=255)
                                                                                                        gTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt                                                                <  1:1434394/98‑1 (MQ=255)
                                                                                                               gCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTg                <  1:245342/139‑1 (MQ=255)
                                                                                                                    gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                            <  2:1206908/42‑1 (MQ=255)
                                                                                                                    gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                                            >  1:1206908/1‑42 (MQ=255)
                                                                                                                    gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgca                                         >  1:1572129/1‑109 (MQ=255)
                                                                                                                    gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgca                                         <  2:1572129/109‑1 (MQ=255)
                                                                                                                    gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGcc           >  2:865974/1‑139 (MQ=255)
                                                                                                                     aTAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCg          >  2:324802/1‑139 (MQ=255)
                                                                                                                         cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                                   >  2:401773/1‑62 (MQ=255)
                                                                                                                         cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa                                                                                   <  1:401773/62‑1 (MQ=255)
                                                                                                                         cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGc                                                                                 <  2:1118394/64‑1 (MQ=255)
                                                                                                                         cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGc                                                                                 >  1:1118394/1‑64 (MQ=255)
                                                                                                                         cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtg      >  2:308969/1‑139 (MQ=255)
                                                                                                                         cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtg      >  2:877622/1‑139 (MQ=255)
                                                                                                                          tCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgc     >  1:637434/1‑139 (MQ=255)
                                                                                                                           cGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgct    <  2:234935/139‑1 (MQ=255)
                                                                                                                             ccATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgctgc  >  1:917094/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                       
CAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGC  >  NC_000913/3398747‑3399010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: