Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,398,875 | C→T | M1M (GTG→ATG) † *368* (TAG→TAA) |
mreD ← mreC ← |
cell wall structural complex MreBCD transmembrane component MreD cell wall structural complex MreBCD transmembrane component MreC |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,398,875 | 0 | C | T | 100.0% | 194.8 / NA | 61 | M1M (GTG→ATG) † *368* (TAG→TAA) | mreD mreC | cell wall structural complex MreBCD transmembrane component MreD cell wall structural complex MreBCD transmembrane component MreC |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (36/25); total (36/25) |
CAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGC > NC_000913/3398747‑3399010 | caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTc > 2:159770/1‑139 (MQ=255) acacCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc > 1:673700/1‑125 (MQ=255) acacCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGcc < 2:673700/125‑1 (MQ=255) cccAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCa > 2:1187277/1‑139 (MQ=255) tttGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg > 2:455937/1‑139 (MQ=255) ggCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTg > 2:874571/1‑139 (MQ=255) gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg > 1:1346575/1‑131 (MQ=255) gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg < 2:1346575/131‑1 (MQ=255) gAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGcac < 1:159770/139‑1 (MQ=255) aaaCAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGcacca > 1:1162093/1‑139 (MQ=255) aGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg < 1:1187277/139‑1 (MQ=255) aGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg < 1:874571/139‑1 (MQ=255) ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggc > 2:1624511/1‑139 (MQ=255) ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggc > 2:1057315/1‑139 (MQ=255) tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGc > 2:928985/1‑113 (MQ=255) tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGc < 1:928985/113‑1 (MQ=255) ccGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa < 2:1162093/139‑1 (MQ=255) ggCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCttt > 1:463788/1‑138 (MQ=255) ggCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCttt < 2:463788/138‑1 (MQ=255) ggCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTtg > 2:114165/1‑139 (MQ=255) tGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg < 1:58350/125‑1 (MQ=255) tGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg > 2:58350/1‑125 (MQ=255) agcaACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAtt < 1:1624511/139‑1 (MQ=255) cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag > 2:968763/1‑139 (MQ=255) cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag < 1:114165/139‑1 (MQ=255) cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTAt < 2:1221320/60‑1 (MQ=255) cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTAt > 1:1221320/1‑60 (MQ=255) aaaGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag > 2:442908/1‑126 (MQ=255) aaaGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTagcag < 1:442908/126‑1 (MQ=255) agagCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgca < 1:1057315/139‑1 (MQ=255) agCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCgg > 2:624170/1‑133 (MQ=255) agCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCgg < 1:624170/133‑1 (MQ=255) gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt < 1:518786/113‑1 (MQ=255) gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt > 2:518786/1‑113 (MQ=255) gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagca > 2:1213223/1‑139 (MQ=255) gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagca > 1:539263/1‑139 (MQ=255) gCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgcagca > 1:663750/1‑139 (MQ=255) cAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg > 1:1188413/1‑85 (MQ=255) cAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg < 2:1188413/85‑1 (MQ=255) cccAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTa > 1:234935/1‑139 (MQ=255) ccAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCTGTAg < 1:968763/139‑1 (MQ=255) cGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc > 2:245342/1‑139 (MQ=255) cGTCCCTGGCTACGATAGCGCGCCATTATTGCCCTCCCCGCACCCGAGCAGGCGGTTGAGCACCACTCTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGCGCCGGCGCAGAAGGCGCacaagaagc > 1:176574/1‑131 (MQ=255) gTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt > 2:1434394/1‑98 (MQ=255) gTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt < 1:1434394/98‑1 (MQ=255) gCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTg < 1:245342/139‑1 (MQ=255) gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg < 2:1206908/42‑1 (MQ=255) gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg > 1:1206908/1‑42 (MQ=255) gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgca > 1:1572129/1‑109 (MQ=255) gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCgca < 2:1572129/109‑1 (MQ=255) gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGcc > 2:865974/1‑139 (MQ=255) aTAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCg > 2:324802/1‑139 (MQ=255) cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa > 2:401773/1‑62 (MQ=255) cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTa < 1:401773/62‑1 (MQ=255) cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGc < 2:1118394/64‑1 (MQ=255) cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGc > 1:1118394/1‑64 (MQ=255) cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtg > 2:308969/1‑139 (MQ=255) cTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtg > 2:877622/1‑139 (MQ=255) tCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgc > 1:637434/1‑139 (MQ=255) cGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgct < 2:234935/139‑1 (MQ=255) ccATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgctgc > 1:917094/1‑139 (MQ=255) | CAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGC > NC_000913/3398747‑3399010 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |