Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,478,802	C→T	*602* (TAG→TAA)	kefB ←	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,478,802	0	C	T	100.0%	174.2 / NA	55	*602* (TAG→TAA)	kefB	potassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/27);  total (28/27)

TGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGC  >  NC_000913/3478674‑3478918                                                                                                                                 |                                                                                                                     tGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAAtt                                                                                                            <  2:787543/139‑1 (MQ=255)            aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTAc                                                                                                                   >  1:1652218/1‑121 (MQ=255)            aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTAc                                                                                                                   <  2:1652218/121‑1 (MQ=255)            aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCaaa                                                                                                              >  1:361001/1‑126 (MQ=255)            aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCaaa                                                                                                              <  2:361001/126‑1 (MQ=255)            aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCg                                                                                                 >  1:440629/1‑139 (MQ=255)                  ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGc                                                                                           >  2:1299740/1‑139 (MQ=255)                    caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTg                                                                                         >  2:1163572/1‑139 (MQ=255)                    caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTg                                                                                         >  1:330599/1‑139 (MQ=255)                           atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcgt                                                                                  >  2:1142124/1‑139 (MQ=255)                             caatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGt                                                                                                >  2:1190809/1‑123 (MQ=255)                             caatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGt                                                                                                <  1:1190809/123‑1 (MQ=255)                             caatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                <  2:440629/139‑1 (MQ=255)                                tattatTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                             <  1:1163572/139‑1 (MQ=255)                                    attTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGc                                                                         >  2:607770/1‑139 (MQ=255)                                     ttttCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTc                                                                                                                 >  1:1662274/1‑98 (MQ=255)                                     ttttCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTc                                                                                                                 <  2:1662274/98‑1 (MQ=255)                                     ttttCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAg                                                                                                    >  1:1038446/1‑111 (MQ=255)                                     ttttCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAg                                                                                                    <  2:1038446/111‑1 (MQ=255)                                        tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTc                                                                                                                 >  1:1571181/1‑95 (MQ=255)                                        tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTc                                                                                                                 <  2:1571181/95‑1 (MQ=255)                                        tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGc                                                                                                   >  2:891527/1‑109 (MQ=255)                                        tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGc                                                                                                   <  1:891527/109‑1 (MQ=255)                                        tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                >  2:1618986/1‑128 (MQ=255)                                        tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                <  1:1618986/128‑1 (MQ=255)                                          gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtc                                                                                    >  2:1406447/1‑122 (MQ=255)                                          gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtc                                                                                    <  1:1406447/122‑1 (MQ=255)                                          gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCa                                                                   >  1:104480/1‑139 (MQ=255)                                                     ccATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                                        <  2:104480/139‑1 (MQ=255)                                                     ccATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                                        <  2:330599/139‑1 (MQ=255)                                                      cATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGa                                                            <  2:1446959/134‑1 (MQ=255)                                                      cATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGa                                                            >  1:1446959/1‑134 (MQ=255)                                                        ttGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAtctc                                                     >  1:155470/1‑139 (MQ=255)                                                          gAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCa                                                                        >  1:754119/1‑118 (MQ=255)                                                          gAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCa                                                                        <  2:754119/118‑1 (MQ=255)                                                             tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCa                                                >  2:494693/1‑139 (MQ=255)                                                                       gACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTgg                                                             >  1:278982/1‑116 (MQ=255)                                                                       gACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTgg                                                             <  2:278982/116‑1 (MQ=255)                                                                                 gCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                                    <  2:140327/131‑1 (MQ=255)                                                                                 gCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                                    >  1:140327/1‑131 (MQ=255)                                                                                       tGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCg                                      <  1:923799/123‑1 (MQ=255)                                                                                       tGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCg                                      >  2:923799/1‑123 (MQ=255)                                                                                       tGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga                      >  2:294329/1‑139 (MQ=255)                                                                                           cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGaaa                  >  1:365775/1‑139 (MQ=255)                                                                                               cAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                             >  2:1434734/1‑76 (MQ=255)                                                                                               cAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACGCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                             <  1:1434734/76‑1 (MQ=255)                                                                                                      aCGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTa       <  2:45249/139‑1 (MQ=255)                                                                                                       cGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTAt      <  1:155356/139‑1 (MQ=255)                                                                                                           ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc  <  2:172651/139‑1 (MQ=255)                                                                                                           ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc  <  2:155470/139‑1 (MQ=255)                                                                                                           ttCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc  <  1:1299740/139‑1 (MQ=255)                                                                                                            tCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGa                                                            <  1:1601096/80‑1 (MQ=255)                                                                                                            tCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGa                                                            >  2:1601096/1‑80 (MQ=255)                                                                                                                   gCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc  <  2:168556/131‑1 (MQ=255)                                                                                                                   gCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc  >  1:168556/1‑131 (MQ=255)                                                                                                                                 |                                                                                                                     TGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGC  >  NC_000913/3478674‑3478918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑