Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,503,952	G→A	*262* (TAG→TAA)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,503,952	0	G	A	100.0%	192.2 / NA	60	*262* (TAG→TAA)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/31);  total (29/31)

GGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACT  >  NC_000913/3503827‑3504083                                                                                                                              |                                                                                                                                    ggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGc                                                                                                                        <  1:183977/139‑1 (MQ=255)      cGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAg                                                                                                                   <  2:382740/139‑1 (MQ=255)           aTTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCt                                                                                                              >  2:1497994/1‑139 (MQ=255)             tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTc                                                                                                            >  2:289851/1‑139 (MQ=255)                ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                         <  2:316971/139‑1 (MQ=255)                     tGCCGGATTCCTTTTCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAAc                                                                                                    >  2:1355270/1‑139 (MQ=255)                     tGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAAc                                                                                                    >  1:69317/1‑139 (MQ=255)                                       cTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTg                                                                                                 <  2:601046/124‑1 (MQ=255)                                       cTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTg                                                                                                 >  1:601046/1‑124 (MQ=255)                                        tGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaa                                                                                 <  1:289851/139‑1 (MQ=255)                                            cTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             <  2:69317/139‑1 (MQ=255)                                            cTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             <  2:496773/139‑1 (MQ=255)                                            cTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             <  1:1497994/139‑1 (MQ=255)                                                     tGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                      <  1:1256418/105‑1 (MQ=255)                                                     tGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                      >  2:1256418/1‑105 (MQ=255)                                                              cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             >  1:1271809/1‑121 (MQ=255)                                                              cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             <  2:1271809/121‑1 (MQ=255)                                                                 aGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAg                                                              <  2:1632578/133‑1 (MQ=255)                                                                 aGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAg                                                              >  1:1632578/1‑133 (MQ=255)                                                                             aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                               >  2:951116/1‑104 (MQ=255)                                                                             aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                               <  1:951116/104‑1 (MQ=255)                                                                             aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa                                                            >  1:1336468/1‑123 (MQ=255)                                                                             aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa                                                            <  2:1336468/123‑1 (MQ=255)                                                                               ggAACGGCGTGCGCGGCGAAAACCATTCATTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCa                                                                                                           >  1:978422/1‑74 (MQ=255)                                                                               ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGt                                                                                                                            >  1:1340594/1‑57 (MQ=255)                                                                               ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGt                                                                                                                            <  2:1340594/57‑1 (MQ=255)                                                                               ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCa                                                                                                           <  2:978422/74‑1 (MQ=255)                                                                                 aaCGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             <  1:652675/102‑1 (MQ=255)                                                                                 aaCGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             >  2:652675/1‑102 (MQ=255)                                                                                      cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACg                                                                                                                             <  1:477883/49‑1 (MQ=255)                                                                                      cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACg                                                                                                                             >  2:477883/1‑49 (MQ=255)                                                                                      cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCtt                                                                                                             <  1:997651/65‑1 (MQ=255)                                                                                      cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCtt                                                                                                             >  2:997651/1‑65 (MQ=255)                                                                                         gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGt                                       <  2:878316/132‑1 (MQ=255)                                                                                         gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGt                                       >  1:878316/1‑132 (MQ=255)                                                                                          cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                    <  1:429131/134‑1 (MQ=255)                                                                                          cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                    >  2:429131/1‑134 (MQ=255)                                                                                               cgAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaa                                                                                 <  2:1518438/84‑1 (MQ=255)                                                                                               cgAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaa                                                                                 >  1:1518438/1‑84 (MQ=255)                                                                                                             tACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTc                                                                                                            >  2:1102220/1‑43 (MQ=255)                                                                                                             tACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTc                                                                                                            <  1:1102220/43‑1 (MQ=255)                                                                                                             tACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                            >  2:1624654/1‑75 (MQ=255)                                                                                                             tACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                            <  1:1624654/75‑1 (MQ=255)                                                                                                              aCCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCtt                                                                                                             >  2:620907/1‑41 (MQ=255)                                                                                                              aCCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCtt                                                                                                             <  1:620907/41‑1 (MQ=255)                                                                                                                cACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                      <  1:995261/126‑1 (MQ=255)                                                                                                                cACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                      >  2:995261/1‑126 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCaa                                                                                                     <  1:912041/40‑1 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCaa                                                                                                     >  2:912041/1‑40 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGc                                                                                      <  2:82488/55‑1 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGc                                                                                      >  1:82488/1‑55 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             >  2:878694/1‑64 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                             <  1:878694/64‑1 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                       >  2:175674/1‑86 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                       <  1:175674/86‑1 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                       >  1:998937/1‑86 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                       <  2:998937/86‑1 (MQ=255)                                                                                                                       ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAct  >  1:214629/1‑139 (MQ=255)                                                                                                                         tGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                               >  2:791835/1‑60 (MQ=255)                                                                                                                         tGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                               <  1:791835/60‑1 (MQ=255)                                                                                                                              |                                                                                                                                    GGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACT  >  NC_000913/3503827‑3504083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑