Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,536,496 T→C Q30R (CAG→CGG)  ompR ← response regulator in two‑component regulatory system with EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,536,4960TC100.0% 272.3 / NA 77Q30R (CAG→CGG) ompRresponse regulator in two‑component regulatory system with EnvZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/36);  total (41/36)

ACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTAAGCGTGTATTC  >  NC_000913/3536369‑3536629
                                                                                                                               |                                                                                                                                     
aCTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCt                                                                                                                               <  2:1540308/136‑1 (MQ=255)
aCTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCt                                                                                                                               >  1:1540308/1‑136 (MQ=255)
 cTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           <  1:206569/139‑1 (MQ=255)
                             cATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacg                                                                                               <  1:1595787/139‑1 (MQ=255)
                              aTCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacgc                                                                                              >  2:1598800/1‑139 (MQ=255)
                               tCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           >  1:103757/1‑109 (MQ=255)
                               tCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           <  2:103757/109‑1 (MQ=255)
                               tCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacgca                                                                                             >  2:666547/1‑139 (MQ=255)
                               tCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacgca                                                                                             <  1:815394/139‑1 (MQ=255)
                                         gTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtc                                                                                   >  1:257811/1‑139 (MQ=255)
                                           aaCATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           >  2:1221705/1‑97 (MQ=255)
                                           aaCATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           <  1:1221705/97‑1 (MQ=255)
                                             cATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg                                                                                         <  1:1284053/129‑1 (MQ=255)
                                             cATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg                                                                                         >  2:1284053/1‑129 (MQ=255)
                                             cATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCa                                                                               >  2:554312/1‑139 (MQ=255)
                                             cATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCa                                                                               >  2:264339/1‑139 (MQ=255)
                                                  aaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCgg                                                                                                                                     <  1:1414337/80‑1 (MQ=255)
                                                  aaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           >  1:460880/1‑90 (MQ=255)
                                                  aaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           <  2:460880/90‑1 (MQ=255)
                                                  aaTCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGac                                                                          <  2:257811/139‑1 (MQ=255)
                                                       aGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGaa                                                                                                                                   <  2:225101/77‑1 (MQ=255)
                                                       aGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGaa                                                                                                                                   >  1:225101/1‑77 (MQ=255)
                                                        gTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAg                                                                    >  2:1331208/1‑139 (MQ=255)
                                                        gTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAg                                                                    >  1:70057/1‑139 (MQ=255)
                                                             aTAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCt                                                               <  1:666547/139‑1 (MQ=255)
                                                                  aTGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAg                                                          <  2:70057/139‑1 (MQ=255)
                                                                     gAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacg                                                                                               >  2:1037390/1‑99 (MQ=255)
                                                                     gAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacg                                                                                               <  1:1037390/99‑1 (MQ=255)
                                                                      aaaGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCg                                                                            <  1:492543/117‑1 (MQ=255)
                                                                      aaaGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCg                                                                            >  2:492543/1‑117 (MQ=255)
                                                                      aaaGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTtct                                                      >  2:1490225/1‑139 (MQ=255)
                                                                        aGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTctct                                                    >  2:1513060/1‑139 (MQ=255)
                                                                        aGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTctct                                                    <  2:333854/139‑1 (MQ=255)
                                                                          aTTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCTAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTg                                                  >  1:217242/1‑139 (MQ=255)
                                                                          aTTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGc                                                                                                                                 >  2:113935/1‑60 (MQ=255)
                                                                          aTTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGc                                                                                                                                 <  1:113935/60‑1 (MQ=255)
                                                                           ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATg                                                                                     <  2:1050009/103‑1 (MQ=255)
                                                                           ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATg                                                                                     >  1:1050009/1‑103 (MQ=255)
                                                                           ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGc                                                 <  2:616974/139‑1 (MQ=255)
                                                                           ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGc                                                 <  1:554312/139‑1 (MQ=255)
                                                                             cACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCAt                                               >  2:1449133/1‑139 (MQ=255)
                                                                                    cagcagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCGCCAGAATCTTGTAGTTCTCTTGc                                                 <  2:56795/130‑1 (MQ=255)
                                                                                    cagcagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCGCCAGAATCTTGTAGTTCTCTTGc                                                 >  1:56795/1‑130 (MQ=255)
                                                                                     agcagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           <  1:934101/55‑1 (MQ=255)
                                                                                     agcagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGtt                                                                                                                           >  2:934101/1‑55 (MQ=255)
                                                                                            gATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGc                                                 >  1:410453/1‑122 (MQ=255)
                                                                                            gATCCATCTGTTCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGc                                                 <  2:410453/122‑1 (MQ=255)
                                                                                               ccATCTGATCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAgg                             >  2:87849/1‑139 (MQ=255)
                                                                                                      ttCTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGcaa                      <  1:1490225/139‑1 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAGGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTAGTCATGGACCACCAGACTCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGcaaca                    >  1:1028933/1‑139 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAg                                                          >  2:999077/1‑101 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAg                                                          <  1:999077/101‑1 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGtt                                                        <  1:1565976/103‑1 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGtt                                                        >  2:1565976/1‑103 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCAt                                               >  2:1291556/1‑112 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCAt                                               <  1:1291556/112‑1 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGcaaca                    >  1:810658/1‑139 (MQ=255)
                                                                                                        cTGCATTAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACACAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGcaaca                    <  2:696546/139‑1 (MQ=255)
                                                                                                              tAGCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGt              <  1:264339/139‑1 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGCAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGACGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACACTTTg               <  1:971023/136‑1 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGac                                                                          <  2:1370118/77‑1 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGac                                                                          >  1:1370118/1‑77 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAg                                                          >  1:222967/1‑93 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAg                                                          <  2:222967/93‑1 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCAttgtttg                                         >  1:75372/1‑110 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCAttgtttg                                         <  2:75372/110‑1 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTg               >  2:971023/1‑136 (MQ=255)
                                                                                                                gCGACGCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTaa            >  2:654379/1‑139 (MQ=255)
                                                                                                                     gCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTa             >  1:21043/1‑133 (MQ=255)
                                                                                                                     gCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTa             <  2:21043/133‑1 (MQ=255)
                                                                                                                     gCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTAAGCgtg       >  2:707283/1‑139 (MQ=255)
                                                                                                                     gCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTAAGCgtg       >  2:882851/1‑139 (MQ=255)
                                                                                                                     gCTTCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTAAGCgtg       >  2:195627/1‑139 (MQ=255)
                                                                                                                        tCGAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTAAGCGTGTAt    <  1:1598800/139‑1 (MQ=255)
                                                                                                                          gAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAg                                                          >  1:1280299/1‑83 (MQ=255)
                                                                                                                          gAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAg                                                          <  2:1280299/83‑1 (MQ=255)
                                                                                                                          gAACCCGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTAAGCGTGTATTc  >  2:1498965/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                     
ACTACGAAGACGTCGGCAAATCGACAAGCCATCTTCACCAGGTAACATTAAATCCAGTACCATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATCTTGTAGTTCTCTTGCATTGTTTGTACTCCCAAAGGTTCGCAACAATTTGTAAGCGTGTATTC  >  NC_000913/3536369‑3536629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑