Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,547,986	C→T	*695* (TAG→TAA)	malQ ←	4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,547,986	0	C	T	100.0%	186.3 / NA	58	*695* (TAG→TAA)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/30);  total (28/30)

GGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTG  >  NC_000913/3547866‑3548118                                                                                                                         |                                                                                                                                     ggTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCa                                                                                                                    >  1:93970/1‑139 (MQ=255)   tGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGc                                                                                                                  <  2:93970/139‑1 (MQ=255)    gATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGct                                                                                                                 <  1:1484734/139‑1 (MQ=255)             acacTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                        <  2:281544/139‑1 (MQ=255)               actgactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCt                                                                                                      >  1:1087741/1‑139 (MQ=255)                 tgactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGt                                                                                                    >  2:87502/1‑139 (MQ=255)                 tgactgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGt                                                                                                    >  2:1584355/1‑139 (MQ=255)                   actgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt                                                                                                                           >  1:258550/1‑114 (MQ=255)                   actgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt                                                                                                                           <  2:258550/114‑1 (MQ=255)                        cGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATc                                                                                             >  1:679725/1‑139 (MQ=255)                             gcggcgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                        <  2:679725/139‑1 (MQ=255)                              cggcgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAg                                                                                       <  2:1634495/139‑1 (MQ=255)                               ggcgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGc                                                                                      <  1:1584355/139‑1 (MQ=255)                                 cgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                      >  2:132025/1‑105 (MQ=255)                                 cgTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                      <  1:132025/105‑1 (MQ=255)                                     aCCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTg                                                                                >  2:495068/1‑139 (MQ=255)                                           ttATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACg                                                                          <  2:1087741/139‑1 (MQ=255)                                             aTCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGcc                                                                        <  1:1642064/139‑1 (MQ=255)                                                          ttCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAAc                                                           <  1:87502/139‑1 (MQ=255)                                                               gATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGa                                                      >  2:747122/1‑139 (MQ=255)                                                                    aTTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCaa                                                 >  2:509985/1‑139 (MQ=255)                                                                             ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGtt                                                                              >  1:1130283/1‑101 (MQ=255)                                                                             ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGtt                                                                              <  2:1130283/101‑1 (MQ=255)                                                                             ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACg                                                                          >  1:654843/1‑105 (MQ=255)                                                                             ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACg                                                                          <  2:654843/105‑1 (MQ=255)                                                                             ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                        >  2:845757/1‑139 (MQ=255)                                                                             ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                        >  2:1477458/1‑139 (MQ=255)                                                                              cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgc                                                                                                             >  2:387581/1‑69 (MQ=255)                                                                              cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgc                                                                                                             <  1:387581/69‑1 (MQ=255)                                                                                 aTAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTg                                                                                                              <  1:599896/65‑1 (MQ=255)                                                                                 aTAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTg                                                                                                              >  2:599896/1‑65 (MQ=255)                                                                                      aCGCGTCAAGCATCGCATCCTGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGAAAAGCttac                               >  1:983500/1‑137 (MQ=255)                                                                                          gTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                        >  2:1503844/1‑78 (MQ=255)                                                                                          gTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                        <  1:1503844/78‑1 (MQ=255)                                                                                            cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                         <  1:495068/139‑1 (MQ=255)                                                                                                   cgcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGttttt                  <  1:792506/139‑1 (MQ=255)                                                                                                    gcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcatca                                                                    <  2:1598237/88‑1 (MQ=255)                                                                                                    gcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcatca                                                                    >  1:1598237/1‑88 (MQ=255)                                                                                                     catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                         >  1:530756/1‑130 (MQ=255)                                                                                                     catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                         <  2:530756/130‑1 (MQ=255)                                                                                                     catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTtat                >  2:1216568/1‑139 (MQ=255)                                                                                                        ccGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCa                                                                            >  2:1391515/1‑76 (MQ=255)                                                                                                        ccGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCa                                                                            <  1:1391515/76‑1 (MQ=255)                                                                                                        ccGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcatca                                                                    >  1:585373/1‑84 (MQ=255)                                                                                                        ccGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcatca                                                                    <  2:585373/84‑1 (MQ=255)                                                                                                         cGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgc                                                                                                             <  1:1424793/42‑1 (MQ=255)                                                                                                         cGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgc                                                                                                             >  2:1424793/1‑42 (MQ=255)                                                                                                            cATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                         >  2:697108/1‑123 (MQ=255)                                                                                                            cATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                         <  1:697108/123‑1 (MQ=255)                                                                                                              tCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                         <  1:734229/121‑1 (MQ=255)                                                                                                              tCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                         >  2:734229/1‑121 (MQ=255)                                                                                                               cAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACt      <  1:1477458/139‑1 (MQ=255)                                                                                                                  ccGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGt   <  1:509985/139‑1 (MQ=255)                                                                                                                   cGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTg  <  1:845757/139‑1 (MQ=255)                                                                                                                     cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGcc                                                                        <  1:891113/67‑1 (MQ=255)                                                                                                                     cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGcc                                                                        >  2:891113/1‑67 (MQ=255)                                                                                                                     cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                          >  2:177969/1‑113 (MQ=255)                                                                                                                     cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                          <  1:177969/113‑1 (MQ=255)                                                                                                                         |                                                                                                                                     GGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTG  >  NC_000913/3547866‑3548118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑