Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,842,455	C→T	*445* (TAG→TAA)	adeQ ←	adenine permease, high affinity, adenine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,842,455	0	C	T	100.0%	237.9 / NA	73	*445* (TAG→TAA)	adeQ	adenine permease, high affinity, adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/37);  total (36/37)

ATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGT  >  NC_000913/3842320‑3842586                                                                                                                                        |                                                                                                                                    aTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa                                                                                                                                  <  1:866128/139‑1 (MQ=255)      ttttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACATACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac                                                                                                                             <  1:1053876/139‑1 (MQ=255)       tttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa                                                                                                                                  >  2:1522393/1‑133 (MQ=255)       tttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTaa                                                                                                                                  <  1:1522393/133‑1 (MQ=255)        ttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                           <  1:586373/139‑1 (MQ=255)          cTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGa                                                                                                                         >  1:851021/1‑139 (MQ=255)                                gAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt                                                                                                                  <  2:417640/124‑1 (MQ=255)                                gAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt                                                                                                                  >  1:417640/1‑124 (MQ=255)                                  aaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                                 <  1:547579/139‑1 (MQ=255)                                         ccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                          <  2:58809/139‑1 (MQ=255)                                               acacTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                    <  1:743960/139‑1 (MQ=255)                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                >  1:646922/1‑139 (MQ=255)                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                <  2:646922/139‑1 (MQ=255)                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                >  2:1428107/1‑139 (MQ=255)                                                         cAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGtt                                                                          >  2:9149/1‑139 (MQ=255)                                                           gCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                        >  2:1358865/1‑107 (MQ=255)                                                           gCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                        <  1:1358865/107‑1 (MQ=255)                                                                        tATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcg                                                           >  1:790531/1‑139 (MQ=255)                                                                          tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         >  1:1314926/1‑139 (MQ=255)                                                                          tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         <  1:1428107/139‑1 (MQ=255)                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTg                                                    <  2:1314926/139‑1 (MQ=255)                                                                                  aTGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt                                                                                                                  <  2:785585/74‑1 (MQ=255)                                                                                  aTGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt                                                                                                                  >  1:785585/1‑74 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                           >  1:793078/1‑63 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccacca                                                                                                                           <  2:793078/63‑1 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac                                                                                                                             >  1:869088/1‑61 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac                                                                                                                             <  2:869088/61‑1 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         >  1:89570/1‑129 (MQ=255)                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         <  2:89570/129‑1 (MQ=255)                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCg                                                                                              <  2:543139/87‑1 (MQ=255)                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCg                                                                                              >  1:543139/1‑87 (MQ=255)                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                <  2:544400/101‑1 (MQ=255)                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                >  1:544400/1‑101 (MQ=255)                                                                                            cTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                >  1:1654163/1‑98 (MQ=255)                                                                                            cTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                <  2:1654163/98‑1 (MQ=255)                                                                                                gTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACtt                                             <  1:102169/129‑1 (MQ=255)                                                                                                gTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACtt                                             >  2:102169/1‑129 (MQ=255)                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac                                                                                                                             <  1:516841/48‑1 (MQ=255)                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATccac                                                                                                                             >  2:516841/1‑48 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCt                                                                                                                  >  2:564846/1‑56 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                >  2:26629/1‑90 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                <  1:26629/90‑1 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc                                                          >  1:1111492/1‑112 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc                                                          <  2:1111492/112‑1 (MQ=255)                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAAGCt                                                                                                                  <  1:564846/56‑1 (MQ=255)                                                                                                         aCTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGGATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGAcct                          >  1:1661855/1‑137 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                     <  2:1118974/124‑1 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                     >  1:1118974/1‑124 (MQ=255)                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAg                      >  2:1000543/1‑139 (MQ=255)                                                                                                               gATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc                    <  2:790531/139‑1 (MQ=255)                                                                                                                  atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                          >  2:61778/1‑114 (MQ=255)                                                                                                                  atCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                          <  1:61778/114‑1 (MQ=255)                                                                                                                    cTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTg               >  1:575094/1‑139 (MQ=255)                                                                                                                     tGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc              >  2:247037/1‑139 (MQ=255)                                                                                                                      ggAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGGGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa             <  1:808696/139‑1 (MQ=255)                                                                                                                       gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                >  1:1658746/1‑71 (MQ=255)                                                                                                                       gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                <  2:1658746/71‑1 (MQ=255)                                                                                                                       gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCAt                                                 >  2:832704/1‑102 (MQ=255)                                                                                                                       gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCAt                                                 <  1:832704/102‑1 (MQ=255)                                                                                                                       gAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa            >  1:228080/1‑139 (MQ=255)                                                                                                                            ccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc                                                          <  2:542903/88‑1 (MQ=255)                                                                                                                            ccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc                                                          >  1:542903/1‑88 (MQ=255)                                                                                                                            ccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         <  2:301211/89‑1 (MQ=255)                                                                                                                            ccTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         >  1:301211/1‑89 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                            >  2:1446445/1‑53 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                            <  1:1446445/53‑1 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgc                                                            >  2:1461408/1‑85 (MQ=255)                                                                                                                             cTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgc                                                            <  1:1461408/85‑1 (MQ=255)                                                                                                                               cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                >  1:664266/1‑63 (MQ=255)                                                                                                                               cGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                <  2:664266/63‑1 (MQ=255)                                                                                                                                 ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         <  1:973734/84‑1 (MQ=255)                                                                                                                                 ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                         >  2:973734/1‑84 (MQ=255)                                                                                                                                 ccAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGt  <  2:228080/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    ATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGT  >  NC_000913/3842320‑3842586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑