Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,853,155 G→A noncoding (103/140 nt) istR ← sRNA antisense regulators IstR‑1 and IstR‑2, affectr tisB, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,853,1550GA98.0% 323.5 / ‑2.5 102noncoding (103/140 nt)istRsRNA antisense regulators IstR‑1 and IstR‑2, affectr tisB, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (51/49);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 102 total aligned reads displayed.

TTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTGCGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTT  >  NC_000913/3853021‑3853288
                                                                                                                                      |                                                                                                                                     
tttATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGc                                                                                                                                   >  2:719536/1‑139 (MQ=255)
         gCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCg                                                                                                                          >  1:138753/1‑139 (MQ=255)
          cacaGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGc                                                                                                                         >  1:1589392/1‑139 (MQ=255)
          cacaGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGc                                                                                                                         >  1:1111649/1‑139 (MQ=255)
              gCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAg                                                                                                                     >  2:512984/1‑139 (MQ=255)
               cTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGc                                                                                                                    >  1:1499932/1‑139 (MQ=255)
                  tACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  2:470414/139‑1 (MQ=255)
                  tACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  1:512984/139‑1 (MQ=255)
                  tACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  1:719536/139‑1 (MQ=255)
                   aCAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGca                                                                                                                   >  2:91323/1‑136 (MQ=255)
                   aCAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGca                                                                                                                   <  1:91323/136‑1 (MQ=255)
                     aGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTg                                                                                                              >  2:1128770/1‑139 (MQ=255)
                        ggAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGtat                                                                                                           <  2:1111649/139‑1 (MQ=255)
                         gagaCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGt                                                                                                                               >  1:1275023/1‑118 (MQ=255)
                         gagaCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGt                                                                                                                               <  2:1275023/118‑1 (MQ=255)
                         gagaCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                      <  2:437667/127‑1 (MQ=255)
                         gagaCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                      >  1:437667/1‑127 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcac                                                                                                                  >  1:1053997/1‑128 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcac                                                                                                                  <  2:1053997/128‑1 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCacac                                                                                                                <  2:216565/130‑1 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCacac                                                                                                                >  1:216565/1‑130 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGtat                                                                                                           <  1:981053/135‑1 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGtat                                                                                                           >  2:981053/1‑135 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATg                                                                                                       >  2:794004/1‑139 (MQ=255)
                            aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATg                                                                                                       >  1:790462/1‑139 (MQ=255)
                              aaTGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTc                                                                                                     <  2:138753/139‑1 (MQ=255)
                                 ggAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTGCGGCTGGTAACCGCAAAGcaca                                                                                                                 >  1:1471845/1‑124 (MQ=255)
                                 ggAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTGCGGCTGGTAACCGCAAAGcaca                                                                                                                 <  2:1471845/124‑1 (MQ=255)
                                   aaaaTTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCacac                                                                                                                >  1:1550839/1‑123 (MQ=255)
                                   aaaaTTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCacac                                                                                                                <  2:1550839/123‑1 (MQ=255)
                                               cAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  2:566987/110‑1 (MQ=255)
                                               cAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 >  1:566987/1‑110 (MQ=255)
                                                            aGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                      <  1:75069/92‑1 (MQ=255)
                                                            aGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                      >  2:75069/1‑92 (MQ=255)
                                                            aGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATAc                                                                                     >  2:862735/1‑125 (MQ=255)
                                                            aGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATAc                                                                                     <  1:862735/125‑1 (MQ=255)
                                                            aGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGa                                                                       >  1:161321/1‑139 (MQ=255)
                                                              gggTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAAc                                                                     >  2:1458608/1‑139 (MQ=255)
                                                                 ttGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAAcgcg                                                                  <  2:1499932/139‑1 (MQ=255)
                                                                   gATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  1:60361/90‑1 (MQ=255)
                                                                   gATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 >  2:60361/1‑90 (MQ=255)
                                                                   gATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  2:570602/90‑1 (MQ=255)
                                                                   gATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 >  1:570602/1‑90 (MQ=255)
                                                                   gATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCa                                                                >  2:917547/1‑139 (MQ=255)
                                                                           tttGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGc                                                        >  2:1013592/1‑139 (MQ=255)
                                                                           tttGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGc                                                        >  2:748553/1‑139 (MQ=255)
                                                                             tGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAGCACTGaa                                                                                            <  1:1095973/101‑1 (MQ=255)
                                                                             tGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGaa                                                                                            >  2:1095973/1‑101 (MQ=255)
                                                                              gTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACt                                                                                               <  2:193539/97‑1 (MQ=255)
                                                                              gTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACt                                                                                               >  1:193539/1‑97 (MQ=255)
                                                                               tACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACt                                                                                               >  2:466947/1‑96 (MQ=255)
                                                                               tACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACt                                                                                               <  1:466947/96‑1 (MQ=255)
                                                                               tACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCAAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  1:609091/78‑1 (MQ=38)
                                                                               tACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCAAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 >  2:609091/1‑78 (MQ=38)
                                                                                      ttGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGa                                             <  1:1013592/139‑1 (MQ=255)
                                                                                       tGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGAc                                            <  1:1458608/139‑1 (MQ=255)
                                                                                       tGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGAc                                            <  1:917547/139‑1 (MQ=255)
                                                                                         cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTaa                                                                                                                             >  2:977544/1‑56 (MQ=255)
                                                                                         cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTaa                                                                                                                             <  1:977544/56‑1 (MQ=255)
                                                                                         cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 >  2:1278262/1‑68 (MQ=255)
                                                                                         cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  1:1278262/68‑1 (MQ=255)
                                                                                         cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGa                                                                       <  1:194261/110‑1 (MQ=255)
                                                                                         cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGa                                                                       >  2:194261/1‑110 (MQ=255)
                                                                                         cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGt                                          >  2:1607532/1‑139 (MQ=255)
                                                                                              aaCAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGa                                                                                             <  2:784022/83‑1 (MQ=255)
                                                                                              aaCAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGa                                                                                             >  1:784022/1‑83 (MQ=255)
                                                                                              aaCAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTa                                     >  1:137643/1‑139 (MQ=255)
                                                                                              aaCAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTa                                     <  2:1589392/139‑1 (MQ=255)
                                                                                                cAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGt                                   >  2:536756/1‑139 (MQ=255)
                                                                                                   aaaaCCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCa                                <  1:1607532/139‑1 (MQ=255)
                                                                                                   aaaaCCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCa                                <  2:137643/139‑1 (MQ=255)
                                                                                                          cgccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 <  1:594691/51‑1 (MQ=255)
                                                                                                          cgccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                                 >  2:594691/1‑51 (MQ=255)
                                                                                                          cgccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTGCGCGCAGAACGCGCAATTTCGGCACGAATTTTGAc                                            <  2:1554679/120‑1 (MQ=255)
                                                                                                          cgccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCcgcg                                                                           >  1:139676/1‑89 (MQ=255)
                                                                                                          cgccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCcgcg                                                                           <  2:139676/89‑1 (MQ=255)
                                                                                                          cgccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGAc                                            >  1:1554679/1‑120 (MQ=255)
                                                                                                          cgccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGa                         >  1:905946/1‑139 (MQ=255)
                                                                                                           gccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGCGCATAg                             >  2:1239126/1‑134 (MQ=255)
                                                                                                           gccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGCGCATAg                             <  1:1239126/134‑1 (MQ=255)
                                                                                                            ccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGt                                          <  2:1458154/120‑1 (MQ=255)
                                                                                                            ccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGt                                          >  1:1458154/1‑120 (MQ=255)
                                                                                                            ccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGt                       <  1:748553/139‑1 (MQ=255)
                                                                                                             cgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTAtt                                       >  2:412284/1‑122 (MQ=255)
                                                                                                             cgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTAtt                                       <  1:412284/122‑1 (MQ=255)
                                                                                                                aGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCa                                                                <  1:1659338/94‑1 (MQ=255)
                                                                                                                aGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCa                                                                >  2:124923/1‑94 (MQ=255)
                                                                                                                aGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCa                                                                <  1:124923/94‑1 (MQ=255)
                                                                                                                aGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCa                                                                >  2:1659338/1‑94 (MQ=255)
                                                                                                                aGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTa                                     <  2:351930/121‑1 (MQ=255)
                                                                                                                aGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTa                                     >  1:351930/1‑121 (MQ=255)
                                                                                                                       tttCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAg            >  1:276952/1‑139 (MQ=255)
                                                                                                                               gTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGtt    <  1:794004/139‑1 (MQ=255)
                                                                                                                               gTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGtt    <  1:536756/139‑1 (MQ=255)
                                                                                                                               gTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGtt    <  2:790462/139‑1 (MQ=255)
                                                                                                                               gTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGtt    <  2:905946/139‑1 (MQ=255)
                                                                                                                               gTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGtt    <  2:161321/139‑1 (MQ=255)
                                                                                                                                tCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTAtt                                       >  2:934492/1‑103 (MQ=255)
                                                                                                                                tCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTAtt                                       <  1:934492/103‑1 (MQ=255)
                                                                                                                                 cGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGtttt  >  1:173495/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TTTATACAAGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTGCGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTT  >  NC_000913/3853021‑3853288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: