| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,926,801 | A→G | V68A (GTA→GCA) | asnC ← | transcriptional activator of asnA, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,926,801 | 0 | A | G | 98.5% | 237.9 / ‑5.0 | 68 | V68A (GTA→GCA) | asnC | transcriptional activator of asnA, autorepressor |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (39/28); total (40/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGC > NC_000913/3926686‑3926934 | gCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt > 1:1440717/1‑135 (MQ=255)gCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTGTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt < 2:1440717/135‑1 (MQ=255) cTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGc > 2:381355/1‑139 (MQ=255) gCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCa > 2:790886/1‑139 (MQ=255) ggTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATa > 2:365713/1‑139 (MQ=255) ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCa < 2:498309/107‑1 (MQ=255) ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCa > 1:498309/1‑107 (MQ=255) ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg < 1:365713/139‑1 (MQ=255) tAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc > 1:1450002/1‑139 (MQ=255) tAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc < 1:790886/139‑1 (MQ=255) ggCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTa > 1:1341388/1‑139 (MQ=255) ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAgctgct > 2:1591493/1‑107 (MQ=255) ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAgctgct < 1:1591493/107‑1 (MQ=255) ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcgcg < 1:447097/128‑1 (MQ=255) ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcgcg > 2:447097/1‑128 (MQ=255) cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTg > 2:972276/1‑113 (MQ=255) cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGCTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGAGCTg < 1:972276/113‑1 (MQ=255) ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGcc > 2:228891/1‑139 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc < 2:895532/119‑1 (MQ=255) atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc > 1:895532/1‑119 (MQ=255) cTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCAttt > 1:1317000/1‑139 (MQ=255) tttCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCAtttt < 2:1317000/139‑1 (MQ=255) ccAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAAc > 1:557099/1‑139 (MQ=255) cgcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCt > 2:1579443/1‑78 (MQ=255) cgcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCt < 1:1579443/78‑1 (MQ=255) aaGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGt > 1:1420164/1‑139 (MQ=255) gcgcTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGc > 2:429557/1‑139 (MQ=255) cgcTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGcc > 1:462191/1‑139 (MQ=255) tctTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCaaa > 2:358/1‑139 (MQ=255) ctTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt < 1:955728/106‑1 (MQ=255) ctTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt > 2:955728/1‑106 (MQ=255) tttAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAAt < 2:1434661/116‑1 (MQ=255) tttAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAAt > 1:1434661/1‑116 (MQ=255) aaTGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt < 1:443480/39‑1 (MQ=255) aaTGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCtt > 2:443480/1‑39 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg < 1:226724/41‑1 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg > 2:226724/1‑41 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGGCATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 1:1182353/1‑139 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc > 2:728308/1‑58 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc < 1:728308/58‑1 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGcccc < 2:313203/61‑1 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGcccc > 1:313203/1‑61 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTaa > 1:1602378/1‑66 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTaa < 2:1602378/66‑1 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGc > 1:1654892/1‑80 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGc < 2:1654892/80‑1 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt > 2:178519/1‑95 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACt < 1:178519/95‑1 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGt > 2:491360/1‑131 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGt < 1:491360/131‑1 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 1:122894/1‑139 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 2:102008/1‑139 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg > 1:1581511/1‑139 (MQ=255) tGCCGATAAAGCAGCCTGCGTCATAAACGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTATTTCGCCAg > 1:131514/1‑139 (MQ=255) gCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGt < 2:1420164/139‑1 (MQ=255) gCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGt < 2:1182353/139‑1 (MQ=255) ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt < 2:462191/139‑1 (MQ=255) ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt > 2:1015766/1‑139 (MQ=255) ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt > 2:620183/1‑139 (MQ=255) cGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTc < 2:1581511/139‑1 (MQ=255) aTAAAGCAGCCTGCGTCATAGCCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg > 1:981349/1‑96 (MQ=255) aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCt > 1:1659896/1‑76 (MQ=255) aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCt < 2:1659896/76‑1 (MQ=255) aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACg < 2:981349/96‑1 (MQ=255) aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCgg > 2:690062/1‑139 (MQ=255) agcCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGc < 1:690062/139‑1 (MQ=255) gcCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc < 1:467585/45‑1 (MQ=255) gcCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc > 2:467585/1‑45 (MQ=255) | GCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGC > NC_000913/3926686‑3926934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |