Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,939,219 G→A *331* (TAG→TAA
S465F (TCT→TTT) 
rbsR →
hsrA ←
transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,939,2190GA100.0% 339.8 / NA 103*331* (TAG→TAA
S465F (TCT→TTT) 
rbsR
hsrA
transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/56);  total (47/56)

Only 100 of 103 total aligned reads displayed.

ACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGT  >  NC_000913/3939088‑3939346
                                                                                                                                     |                                                                                                                               
aCTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  2:111976/139‑1 (MQ=255)
aCTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  2:1159172/139‑1 (MQ=255)
aCTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  1:1330881/139‑1 (MQ=255)
    tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGt                                                                                                                      >  2:638524/1‑139 (MQ=255)
    tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGt                                                                                                                      >  2:497958/1‑139 (MQ=255)
           aCCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGa                                                                                                               >  1:1327325/1‑139 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              <  1:351682/139‑1 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              <  2:1327325/139‑1 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              <  2:312957/139‑1 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              >  2:417056/1‑139 (MQ=255)
                       aCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAACAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                        >  1:1005245/1‑118 (MQ=255)
                       aCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAACAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                        <  2:1005245/118‑1 (MQ=255)
                          gggggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                <  1:1436500/139‑1 (MQ=255)
                           ggggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                         >  2:1029309/1‑113 (MQ=255)
                           ggggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                         <  1:1029309/113‑1 (MQ=255)
                            gggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCg                                                                                              >  1:1060202/1‑139 (MQ=255)
                             ggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                             <  2:1060202/139‑1 (MQ=255)
                                gCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttg                                                                                          >  2:1226881/1‑139 (MQ=255)
                                 cTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  2:407023/106‑1 (MQ=255)
                                 cTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          >  1:407023/1‑106 (MQ=255)
                                   ggCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                             >  2:1247676/1‑133 (MQ=255)
                                   ggCGATTGATGTACTAATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                             <  1:1247676/133‑1 (MQ=255)
                                     cGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTc                                                                                     >  1:104913/1‑139 (MQ=255)
                                      gATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    <  1:497958/139‑1 (MQ=255)
                                       aTTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAg                                                                                   <  1:1226881/139‑1 (MQ=255)
                                           aTGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttgt                                                                                         >  1:625055/1‑129 (MQ=255)
                                           aTGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttgt                                                                                         <  2:625055/129‑1 (MQ=255)
                                                cTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGaa                                                                          <  2:560630/139‑1 (MQ=255)
                                                  catccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          >  1:284127/1‑89 (MQ=255)
                                                  catccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  2:284127/89‑1 (MQ=255)
                                                   atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              >  2:651790/1‑100 (MQ=255)
                                                   atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              <  1:651790/100‑1 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                             <  1:1630922/115‑1 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                             >  2:1630922/1‑115 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                     >  2:13935/1‑139 (MQ=255)
                                                        tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctg                                                                  >  2:1216636/1‑139 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                             <  2:1620265/89‑1 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                             >  1:1620265/1‑89 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           <  2:1323052/139‑1 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           <  2:104913/139‑1 (MQ=255)
                                                                cccAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          >  2:982707/1‑75 (MQ=255)
                                                                cccAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  1:982707/75‑1 (MQ=255)
                                                                  cAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAAc                                                        <  2:168641/139‑1 (MQ=255)
                                                                   aGCCGACCCTTCAGCAACAACTATTACAACTTACTCTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                             <  1:988440/103‑1 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAAttt                                                                                                                             >  2:680786/1‑67 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAAttt                                                                                                                             <  1:680786/67‑1 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  1:615921/70‑1 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          >  2:615921/1‑70 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              >  2:579981/1‑82 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                              <  1:579981/82‑1 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    <  2:1517020/108‑1 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    >  1:1517020/1‑108 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    >  2:1311329/1‑108 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    <  1:1311329/108‑1 (MQ=255)
                                                                      cGACCCTTCAGCAACAACGATTGCAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           <  1:1078612/132‑1 (MQ=255)
                                                                      cGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCACGAACATTGCTGCTGAAGc                                                           >  2:1078612/1‑132 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAatta                                                                                                       <  1:767317/84‑1 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAatta                                                                                                       >  2:767317/1‑84 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                         >  1:1000511/1‑130 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                         <  2:1000511/130‑1 (MQ=255)
                                                                               aGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           <  2:580772/123‑1 (MQ=255)
                                                                                      acGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    >  1:50805/1‑91 (MQ=255)
                                                                                      acGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    <  2:50805/91‑1 (MQ=255)
                                                                                      acGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                      >  2:874128/1‑121 (MQ=255)
                                                                                      acGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                      <  1:874128/121‑1 (MQ=255)
                                                                                       cGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGAt                                   <  1:1435567/139‑1 (MQ=255)
                                                                                         aTTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           >  2:955940/1‑113 (MQ=255)
                                                                                         aTTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           <  1:955940/113‑1 (MQ=255)
                                                                                         aTTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaa                                 <  1:38524/139‑1 (MQ=255)
                                                                                           tACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAAc                               >  2:1454211/1‑139 (MQ=255)
                                                                                            aCAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                              >  2:214555/1‑139 (MQ=255)
                                                                                            aCAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                              <  1:1454211/139‑1 (MQ=255)
                                                                                              aaCTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                    >  1:59981/1‑131 (MQ=255)
                                                                                              aaCTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                    <  2:59981/131‑1 (MQ=255)
                                                                                                cTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGtata                          <  2:1598929/139‑1 (MQ=255)
                                                                                                   a‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCtt                                                                                                                   <  1:836425/47‑1 (MQ=255)
                                                                                                   a‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCtt                                                                                                                   >  2:836425/1‑47 (MQ=255)
                                                                                                   a‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTg                       >  2:1294771/1‑139 (MQ=255)
                                                                                                      cTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          <  1:1403539/39‑1 (MQ=255)
                                                                                                      cTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                          >  2:1403539/1‑39 (MQ=255)
                                                                                                            ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                      >  2:448347/1‑101 (MQ=255)
                                                                                                            ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                      <  1:448347/101‑1 (MQ=255)
                                                                                                            ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                              <  2:1541311/125‑1 (MQ=255)
                                                                                                            ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                              >  1:1541311/1‑125 (MQ=255)
                                                                                                            ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGt                >  1:1155476/1‑139 (MQ=255)
                                                                                                             tCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGtt               <  1:417056/139‑1 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                             >  1:1140069/1‑41 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                             <  2:1140069/41‑1 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctgc                                                                 >  1:263883/1‑85 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctgc                                                                 <  2:263883/85‑1 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           <  1:877532/91‑1 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           >  2:877532/1‑91 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGAc           <  2:1155476/139‑1 (MQ=255)
                                                                                                                 aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGAc           <  1:214555/139‑1 (MQ=255)
                                                                                                                      aCGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg      <  2:206770/139‑1 (MQ=255)
                                                                                                                          ggTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgt  <  1:1294771/139‑1 (MQ=255)
                                                                                                                               ggCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    <  1:959850/52‑1 (MQ=255)
                                                                                                                               ggCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                    >  2:959850/1‑52 (MQ=255)
                                                                                                                               ggCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           >  1:136059/1‑77 (MQ=255)
                                                                                                                               ggCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                           <  2:136059/77‑1 (MQ=255)
                                                                                                                                     |                                                                                                                               
ACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTA‑‑CTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGT  >  NC_000913/3939088‑3939346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: