Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,077	G→A	*349* (TAG→TAA)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,077	0	G	A	100.0%	290.7 / NA	89	*349* (TAG→TAA)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/45);  total (44/45)

CGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGG  >  NC_000913/3969955‑3970202                                                                                                                            |                                                                                                                              cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt                                                                                                                >  2:302775/1‑139 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt                                                                                                                <  2:546699/139‑1 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt                                                                                                                >  1:46476/1‑139 (MQ=255)     cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                            >  2:1513685/1‑123 (MQ=255)     cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                            <  1:1513685/123‑1 (MQ=255)           aagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAg                                                                                                      >  2:324964/1‑139 (MQ=255)                ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                                                                 <  1:1199484/139‑1 (MQ=255)                ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                                                                                 >  2:1199484/1‑139 (MQ=255)                  ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcg                                                                                               >  2:1278935/1‑139 (MQ=255)                       aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                          <  2:46476/139‑1 (MQ=255)                        aCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCtt                                                                                         >  1:1306702/1‑139 (MQ=255)                               aGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGGGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                  <  1:321595/139‑1 (MQ=255)                                   cACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAgaga                                                                              >  1:565907/1‑139 (MQ=255)                                          tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                       <  1:1278935/139‑1 (MQ=255)                                          tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                       >  2:525654/1‑139 (MQ=255)                                          tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                       >  2:115339/1‑139 (MQ=255)                                            ccTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                                                                  >  2:841046/1‑94 (MQ=255)                                            ccTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                                                                  <  1:841046/94‑1 (MQ=255)                                            ccTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg                                                                     <  1:1091316/139‑1 (MQ=255)                                               tCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                          >  2:1084653/1‑115 (MQ=255)                                               tCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                          <  1:1084653/115‑1 (MQ=255)                                                 cTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTa                                                                                        <  1:1596991/115‑1 (MQ=255)                                                 cTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTa                                                                                        >  2:1596991/1‑115 (MQ=255)                                                  tgatgaTTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                               <  2:565907/139‑1 (MQ=255)                                                     tgatTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                            <  1:115339/139‑1 (MQ=255)                                                       atTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac                                                          <  1:1547593/139‑1 (MQ=255)                                                       atTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac                                                          <  1:302775/139‑1 (MQ=255)                                                       atTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac                                                          <  1:525654/139‑1 (MQ=255)                                                         tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                                                                         >  2:360742/1‑122 (MQ=255)                                                         tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                                                                         <  1:360742/122‑1 (MQ=255)                                                         tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg                                                        >  1:931113/1‑139 (MQ=255)                                                           tgcggggCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa                                                      <  1:1244829/136‑1 (MQ=255)                                                               gggCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt                                                                <  1:140629/125‑1 (MQ=255)                                                               gggCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt                                                                >  2:140629/1‑125 (MQ=255)                                                                  cATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                            >  2:638080/1‑126 (MQ=255)                                                                  cATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg                                                            <  1:638080/126‑1 (MQ=255)                                                                   aTTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAc                                              >  1:576447/1‑139 (MQ=255)                                                                   aTTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAc                                              <  2:1306702/139‑1 (MQ=255)                                                                          ggggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                       <  1:1148736/107‑1 (MQ=255)                                                                          ggggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                       >  2:1148736/1‑107 (MQ=255)                                                                          ggggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCg                                       >  2:900853/1‑139 (MQ=255)                                                                            ggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                            <  2:300807/52‑1 (MQ=255)                                                                            ggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                            >  1:300807/1‑52 (MQ=255)                                                                            ggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                  >  2:1431328/1‑94 (MQ=255)                                                                            ggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                  <  1:1431328/94‑1 (MQ=255)                                                                             gggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGaa                                    >  2:529414/1‑139 (MQ=255)                                                                               gCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                                                                  >  2:1331361/1‑59 (MQ=255)                                                                               gCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                                                                  <  1:1331361/59‑1 (MQ=255)                                                                               gCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGc                                  >  1:611878/1‑139 (MQ=255)                                                                                 tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                                                                  >  1:1013900/1‑57 (MQ=255)                                                                                 tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                                                                  <  2:1013900/57‑1 (MQ=255)                                                                                 tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAgg                                                                                                     >  1:1082151/1‑70 (MQ=255)                                                                                 tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAgg                                                                                                     <  2:1082151/70‑1 (MQ=255)                                                                                      ggggCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAg                           <  1:324964/139‑1 (MQ=255)                                                                                         gCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                          >  1:785556/1‑73 (MQ=255)                                                                                         gCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                          <  2:785556/73‑1 (MQ=255)                                                                                          cTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATgg                       <  1:782609/139‑1 (MQ=255)                                                                                             gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgc                                                                                           >  2:839039/1‑68 (MQ=255)                                                                                             gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgc                                                                                           <  1:839039/68‑1 (MQ=255)                                                                                             gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac                                                          <  2:712170/101‑1 (MQ=255)                                                                                             gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac                                                          >  1:712170/1‑101 (MQ=255)                                                                                             gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGgcgc                    >  1:145547/1‑139 (MQ=255)                                                                                                  gCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCa                                                                                                         <  1:866934/49‑1 (MQ=255)                                                                                                  gCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCa                                                                                                         >  2:866934/1‑49 (MQ=255)                                                                                                  gCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTg               >  2:588577/1‑139 (MQ=255)                                                                                                     ttAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTg            <  2:576447/139‑1 (MQ=255)                                                                                                       aaCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCa          >  1:1214587/1‑139 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                            <  1:1646232/56‑1 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                            >  2:1646232/1‑56 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg                                                                     <  1:662675/79‑1 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg                                                                     >  2:662675/1‑79 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact                                                         >  2:902816/1‑91 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact                                                         <  1:902816/91‑1 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                           >  1:1209627/1‑89 (MQ=255)                                                                                                        aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                           <  2:1209627/89‑1 (MQ=255)                                                                                                          ccgccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                               >  1:512059/1‑99 (MQ=255)                                                                                                          ccgccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                               <  2:512059/99‑1 (MQ=255)                                                                                                           cgccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCg      <  1:588577/139‑1 (MQ=255)                                                                                                              cgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt                 <  2:957523/125‑1 (MQ=255)                                                                                                              cgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGAGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt                 >  1:957523/1‑125 (MQ=255)                                                                                                               gTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCAATCAAGATGGCGCCGTTGGTGCATGCGTTgg  <  1:900853/139‑1 (MQ=255)                                                                                                                  gCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGgc                                                                                                    <  2:1412179/38‑1 (MQ=255)                                                                                                                  gCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGgc                                                                                                    >  1:1412179/1‑38 (MQ=255)                                                                                                                  gCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                          <  2:1567007/48‑1 (MQ=255)                                                                                                                  gCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                          >  1:1567007/1‑48 (MQ=255)                                                                                                                  gCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact                                                         >  2:1319764/1‑81 (MQ=255)                                                                                                                  gCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgact                                                         <  1:1319764/81‑1 (MQ=255)                                                                                                                   cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                                                                           >  2:604820/1‑62 (MQ=255)                                                                                                                   cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                                                                           <  1:604820/62‑1 (MQ=255)                                                                                                                            |                                                                                                                              CGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGG  >  NC_000913/3969955‑3970202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑