Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,075,454	G→A	*262* (TAG→TAA)	yihW →	putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,075,454	0	G	A	100.0%	224.3 / NA	69	*262* (TAG→TAA)	yihW	putative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/35);  total (34/35)

ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTG  >  NC_000913/4075318‑4075586                                                                                                                                         |                                                                                                                                     aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGATCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                                    >  1:259949/1‑139 (MQ=255)     ttCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAttttt                                                                                                                                >  2:939678/1‑139 (MQ=255)       cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                              >  1:1302132/1‑139 (MQ=255)           ggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGAcc                                                                                                                          <  2:786452/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                   <  1:1572484/139‑1 (MQ=255)                   aaaTTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCt                                                                                                                  <  1:920704/139‑1 (MQ=255)                                       gTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCt                                                                                                      <  1:315556/131‑1 (MQ=255)                                       gTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCt                                                                                                      >  2:315556/1‑131 (MQ=255)                                          aCTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                           <  2:1302132/139‑1 (MQ=255)                                                ctctTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAAcc                                                                                     <  2:581240/139‑1 (MQ=255)                                                ctctTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAAcc                                                                                     <  2:55442/139‑1 (MQ=255)                                                   tttACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCAt                                                                                  >  1:613792/1‑139 (MQ=255)                                                      aCCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCt                                                                               >  1:1440772/1‑139 (MQ=255)                                                          acgaGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTcc                                                                           >  1:756797/1‑139 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                        <  2:1440772/139‑1 (MQ=255)                                                             aGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGc                                                                        >  2:329982/1‑139 (MQ=255)                                                                 gccgccCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                              >  1:1658393/1‑81 (MQ=255)                                                                 gccgccCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                              <  2:1658393/81‑1 (MQ=255)                                                                 gccgccCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                           >  2:1166112/1‑116 (MQ=255)                                                                 gccgccCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGc                                                                                           <  1:1166112/116‑1 (MQ=255)                                                                            cgcTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCtt                                                         <  1:939678/139‑1 (MQ=255)                                                                                      aCGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGt                                                                  >  1:1249428/1‑120 (MQ=255)                                                                                      aCGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGt                                                                  <  2:1249428/120‑1 (MQ=255)                                                                                       cGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtg                                                                                                                               <  2:764957/58‑1 (MQ=255)                                                                                       cGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtg                                                                                                                               >  1:764957/1‑58 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                              >  1:408317/1‑45 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                              <  2:408317/45‑1 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCt                                                                                                                  <  2:288922/57‑1 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCt                                                                                                                  >  1:288922/1‑57 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCtt                                                                                                     >  1:1352236/1‑70 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCtt                                                                                                     <  2:1352236/70‑1 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                                 <  2:1011133/138‑1 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                                 >  1:1011133/1‑138 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                   >  2:208137/1‑55 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                                   <  1:208137/55‑1 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGct                               >  2:637586/1‑139 (MQ=255)                                                                                                       aaaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctc                              <  1:1325059/139‑1 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctct                             >  1:319720/1‑139 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctct                             <  2:319720/139‑1 (MQ=255)                                                                                                          ttGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtg                                                        >  2:355786/1‑110 (MQ=255)                                                                                                          ttGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtg                                                        <  1:355786/110‑1 (MQ=255)                                                                                                              aaTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt                       <  2:1207988/139‑1 (MQ=255)                                                                                                              aaTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt                       <  1:637586/139‑1 (MQ=255)                                                                                                              aaTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCt                       <  1:329982/139‑1 (MQ=255)                                                                                                                   ttcttcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                         >  1:207631/1‑116 (MQ=255)                                                                                                                   ttcttcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                         <  2:207631/116‑1 (MQ=255)                                                                                                                    tcttcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAtt                 >  1:1207839/1‑139 (MQ=255)                                                                                                                       tcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCa                                                                                                           <  2:348937/46‑1 (MQ=255)                                                                                                                       tcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCa                                                                                                           >  1:348937/1‑46 (MQ=255)                                                                                                                       tcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat                           <  2:1212567/126‑1 (MQ=255)                                                                                                                       tcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat                           >  1:1212567/1‑126 (MQ=255)                                                                                                                         cccAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGt            <  2:1207839/139‑1 (MQ=255)                                                                                                                              gAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAACATGCTCTATATCTTTGATTTAGGTAAtgt       >  2:378218/1‑139 (MQ=255)                                                                                                                              gAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTaa          <  1:1090485/136‑1 (MQ=255)                                                                                                                              gAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACATTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTaa          >  2:1090485/1‑136 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTa               >  2:1269862/1‑127 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTa               <  1:1269862/127‑1 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGAtt   >  1:96325/1‑139 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcac                                                                                                >  2:348381/1‑45 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcac                                                                                                <  1:348381/45‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGcc                                                                                          <  2:836485/51‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGcc                                                                                          >  1:836485/1‑51 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCAt                                                                                  >  2:568000/1‑59 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCAt                                                                                  <  1:568000/59‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                                      >  1:147973/1‑71 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                                      <  2:147973/71‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtgt                                                       >  1:92887/1‑86 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtgt                                                       <  2:92887/86‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTg  >  1:860101/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTG  >  NC_000913/4075318‑4075586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑