Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,290,119 C→T C104C (TGC→TGT nrfC → formate‑dependent nitrite reductase, 4Fe4S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,290,1190CT99.0% 317.4 / NA 98C104C (TGC→TGTnrfCformate‑dependent nitrite reductase, 4Fe4S subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (49/48);  total (50/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGACGGC  >  NC_000913/4289986‑4290253
                                                                                                                                     |                                                                                                                                      
aCAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGAc                                                                                                                                   <  2:100474/139‑1 (MQ=255)
    ggTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGt                                                                                                                               >  1:1187711/1‑139 (MQ=255)
      tGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGc                                                                                                                             >  1:421843/1‑139 (MQ=255)
         tggAAGGCGTCTCGCGCCTGACGATTATTCGGAGCGAGCCGCAGGTCGAATTGACTGATGAGAAAGATCTAGTCTTCGGTAAGGCTTTCGAGCACTGCGATCATGCGCCGGGCGTGGACGTCTGTGCGACCGGTGAGTc                                                                                                                          >  1:923465/2‑139 (MQ=255)
              ggCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTc                                                                                                                     <  2:421843/139‑1 (MQ=255)
               gCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcg                                                                                                                    >  2:1508976/1‑139 (MQ=255)
               gCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcg                                                                                                                    <  2:97982/139‑1 (MQ=255)
                cGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcgc                                                                                                                   >  1:142516/1‑139 (MQ=255)
                  tctcGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTc                                                                                                                     <  2:541070/135‑1 (MQ=255)
                  tctcGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTc                                                                                                                     >  1:541070/1‑135 (MQ=255)
                  tctcGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGa                                                                                                                 <  2:142516/139‑1 (MQ=255)
                  tctcGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGa                                                                                                                 >  2:874377/1‑139 (MQ=255)
                    tcGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtg                                                                                                               <  1:350406/139‑1 (MQ=255)
                      gcgcCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             <  1:577787/139‑1 (MQ=255)
                        gcCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgctgc                                                                                                           <  1:1551090/139‑1 (MQ=255)
                                         aGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             <  2:211906/120‑1 (MQ=255)
                                         aGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             >  1:211906/1‑120 (MQ=255)
                                         aGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAg                                                                                                        >  1:870144/1‑125 (MQ=255)
                                         aGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAg                                                                                                        <  2:870144/125‑1 (MQ=255)
                                         aGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATg                                                                                          >  1:290271/1‑139 (MQ=255)
                                           cGAGCCGTAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGtt                                                                                        >  1:124988/1‑139 (MQ=255)
                                              gCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTg                                                                                                      <  1:1083165/122‑1 (MQ=255)
                                              gCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTg                                                                                                      >  2:1083165/1‑122 (MQ=255)
                                              gCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAAt                                                                                     <  1:874377/139‑1 (MQ=255)
                                               ccGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGa                                                                                                   <  1:199586/124‑1 (MQ=255)
                                               ccGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGa                                                                                                   >  2:199586/1‑124 (MQ=255)
                                                      gCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGAtct                                                                             <  2:1187711/139‑1 (MQ=255)
                                                              ccTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  2:290271/139‑1 (MQ=255)
                                                                 gATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGtt                                                                  <  2:124988/139‑1 (MQ=255)
                                                                  aTGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGa                                                                                                                 <  2:747375/91‑1 (MQ=255)
                                                                  aTGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGa                                                                                                                 >  1:747375/1‑91 (MQ=255)
                                                                       aaaTATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     >  2:77261/1‑130 (MQ=255)
                                                                       aaaTATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  1:77261/130‑1 (MQ=255)
                                                                       aaaTATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGt                                                            >  1:724835/1‑139 (MQ=255)
                                                                            tCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcg                                                                                                                    >  1:987186/1‑78 (MQ=255)
                                                                            tCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcg                                                                                                                    <  2:987186/78‑1 (MQ=255)
                                                                            tCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTc                                                                                                                     <  1:570648/77‑1 (MQ=255)
                                                                            tCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTc                                                                                                                     >  2:570648/1‑77 (MQ=255)
                                                                               cttcttCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTc                                                                                                                     >  1:935349/1‑74 (MQ=255)
                                                                               cttcttCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTc                                                                                                                     <  2:935349/74‑1 (MQ=255)
                                                                               cttcttCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATc                                                    >  1:1347716/1‑139 (MQ=255)
                                                                                  cttcCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgcc                                                 >  2:719786/1‑139 (MQ=255)
                                                                                    tcCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCg                                                                        <  2:1400498/114‑1 (MQ=255)
                                                                                    tcCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCg                                                                        >  1:1400498/1‑114 (MQ=255)
                                                                                      cGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcg                                                                                                                    <  2:1654721/68‑1 (MQ=255)
                                                                                      cGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTcg                                                                                                                    >  1:1654721/1‑68 (MQ=255)
                                                                                             cTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTa                                      >  2:1571395/1‑139 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGa                                                                                                   >  2:401827/1‑76 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGa                                                                                                   <  1:401827/76‑1 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATg                                                                                          <  1:1556621/85‑1 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATg                                                                                          >  2:1556621/1‑85 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGAt                                                                               <  2:1344228/96‑1 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGAt                                                                               >  1:1344228/1‑96 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  2:132778/127‑1 (MQ=255)
                                                                                               tGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                >  1:132778/1‑127 (MQ=255)
                                                                                                gCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTggg                                                                                                    >  1:1529301/1‑74 (MQ=255)
                                                                                                gCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTggg                                                                                                    <  2:1529301/74‑1 (MQ=255)
                                                                                                gCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACcg                                   >  2:387193/1‑139 (MQ=255)
                                                                                                  cAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACcgcg                                 <  1:1659902/139‑1 (MQ=255)
                                                                                                  cAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACcgcg                                 <  2:724835/139‑1 (MQ=255)
                                                                                                   aGCACTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGt                                <  1:66134/139‑1 (MQ=255)
                                                                                                      aCTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             >  1:556099/1‑59 (MQ=255)
                                                                                                      aCTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             <  2:556099/59‑1 (MQ=255)
                                                                                                      aCTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGt                                                                >  1:954350/1‑104 (MQ=255)
                                                                                                      aCTGCGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGt                                                                <  2:954350/104‑1 (MQ=255)
                                                                                                          cGATCATGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCgccgcc                                              <  2:996234/118‑1 (MQ=255)
                                                                                                          cGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCgccgcc                                              >  1:996234/1‑118 (MQ=255)
                                                                                                                tGCGCCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                >  2:1536877/1‑110 (MQ=255)
                                                                                                                tGCGCCGTGCATTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  1:1536877/110‑1 (MQ=255)
                                                                                                                  cgcCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             <  2:724534/47‑1 (MQ=255)
                                                                                                                  cgcCGTGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGAtgct                                                                                                             >  1:724534/1‑47 (MQ=255)
                                                                                                                       tGCGTTGGCGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  2:1399900/103‑1 (MQ=255)
                                                                                                                       tGCGTTGGCGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                >  1:1399900/1‑103 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  2:286293/82‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     >  2:2066/1‑82 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  2:930951/82‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     >  1:930951/1‑82 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  1:2066/82‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     >  2:53985/1‑82 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     <  1:53985/82‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCg                                                                     >  1:286293/1‑82 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGt                                                                <  1:84955/87‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGt                                                                >  2:84955/1‑87 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTTAGTACTGCATCGCCGCCTGTCCGTACcgcg                                 >  2:1230887/1‑118 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTTAGTACTGCATCGCCGCCTGTCCGTACcgcg                                 <  1:1230887/118‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                >  1:582029/1‑103 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  2:582029/103‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTatccatc                  >  2:1094964/1‑133 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTatccatc                  <  1:1094964/133‑1 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCa            >  1:863845/1‑139 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCa            >  2:354786/1‑139 (MQ=255)
                                                                                                                       tGCGTTGACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCa            >  2:1388952/1‑139 (MQ=255)
                                                                                                                             gACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTatccatc                  >  1:424264/1‑127 (MQ=255)
                                                                                                                             gACGTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTatccatc                  <  2:424264/127‑1 (MQ=255)
                                                                                                                                gTCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGAcgg   <  1:354786/139‑1 (MQ=255)
                                                                                                                                 tCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                <  1:228941/93‑1 (MQ=255)
                                                                                                                                 tCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATcgccg                                                >  2:228941/1‑93 (MQ=255)
                                                                                                                                 tCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGAcggc  >  1:133782/1‑139 (MQ=255)
                                                                                                                                 tCTGTCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCCTTATCCATCCGGTCACGAAGAcggc  >  1:455318/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                      
ACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTTCCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGGTGCGTCTTTTCGCGATGCTGCCAGTGGGATTGTCGATGTTAATCCGGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCGCCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGACGGC  >  NC_000913/4289986‑4290253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: