Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,051 G→A D118N (GAC→AAC)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,0510GA97.3% 229.0 / ‑2.0 74D118N (GAC→AAC) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (37/35);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

CCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTG  >  NC_000913/4423917‑4424188
                                                                                                                                      |                                                                                                                                         
ccGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAacta                                                                                                                                       <  2:761240/139‑1 (MQ=255)
       tGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCttt                                                                                                                                >  1:850347/1‑139 (MQ=255)
       tGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCttt                                                                                                                                >  1:1522534/1‑139 (MQ=255)
       tGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCttt                                                                                                                                >  2:1624624/1‑139 (MQ=255)
           aCGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCg                                                                                                                            >  1:769773/1‑139 (MQ=255)
              cATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACa                                                                                                                         >  2:1605205/1‑139 (MQ=255)
                tctcGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACAtt                                                                                                                       <  2:1230790/139‑1 (MQ=255)
                 ctcGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTc                                                                                                                      >  2:48539/1‑139 (MQ=255)
                          tACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACAtt                                                                                                                       >  2:171430/1‑129 (MQ=255)
                          tACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACAtt                                                                                                                       <  1:171430/129‑1 (MQ=255)
                           aCCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTAcgcg                                                                                                            <  2:1522534/139‑1 (MQ=255)
                           aCCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTAcgcg                                                                                                            <  1:1624624/139‑1 (MQ=255)
                                  ttACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACAtt                                                                                                                       >  2:1220220/1‑121 (MQ=255)
                                  ttACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACAtt                                                                                                                       <  1:1220220/121‑1 (MQ=255)
                                  ttACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTa                                                                                                     >  2:1125924/1‑139 (MQ=255)
                                  ttACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTa                                                                                                     >  2:544531/1‑139 (MQ=255)
                                         tCGCTTGGTGGCATTGTCCACACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaag                                                                                              >  1:981943/1‑139 (MQ=255)
                                            cTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGaagaag                                                                                           <  1:48539/139‑1 (MQ=255)
                                                  ggCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGc                                                                                     <  1:1125924/139‑1 (MQ=255)
                                                      ttGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 <  2:1272429/139‑1 (MQ=255)
                                                      ttGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 <  2:981943/139‑1 (MQ=255)
                                                                 cactccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                      <  2:502283/139‑1 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAg                                                                                                   <  2:18540/108‑1 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAg                                                                                                   >  1:18540/1‑108 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                            >  2:1483568/1‑115 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                            >  2:750711/1‑115 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                            >  2:319505/1‑115 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                            <  1:750711/115‑1 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                            <  1:319505/115‑1 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                            <  1:1483568/115‑1 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                                    >  2:72997/1‑139 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                                    >  2:1539083/1‑139 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                                    >  1:1374609/1‑139 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                                    >  2:287756/1‑139 (MQ=255)
                                                                   ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                                    >  2:587261/1‑139 (MQ=255)
                                                                    tccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTAcgcg                                                                                                            <  1:1308852/98‑1 (MQ=255)
                                                                    tccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTAcgcg                                                                                                            >  2:1308852/1‑98 (MQ=255)
                                                                     ccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactgaa                                                                  <  1:937694/139‑1 (MQ=255)
                                                                        ctcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAacac                                                               >  2:879868/1‑139 (MQ=255)
                                                                         tcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACAcc                                                              >  1:267408/1‑139 (MQ=255)
                                                                              ccACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCaa                                                         >  1:723259/1‑139 (MQ=255)
                                                                                  cGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                            <  1:1393666/116‑1 (MQ=255)
                                                                                  cGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                            >  2:1393666/1‑116 (MQ=255)
                                                                                    cATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGAt                                                   <  2:769773/139‑1 (MQ=255)
                                                                                       gggCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTAt                                                >  1:791632/1‑139 (MQ=255)
                                                                                       gggCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTAt                                                >  1:1418157/1‑139 (MQ=255)
                                                                                        ggCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGc                                                           <  2:1054061/127‑1 (MQ=255)
                                                                                        ggCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGAGCAGGGCGAGTATGAACTGAACACCGGc                                                           >  1:1054061/1‑127 (MQ=255)
                                                                                         gcgcAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCg                                              <  1:600100/139‑1 (MQ=255)
                                                                                            cAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTAcgc                                                                                                             >  2:383069/1‑73 (MQ=255)
                                                                                            cAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTAcgc                                                                                                             <  1:383069/73‑1 (MQ=255)
                                                                                                   ggCTGGCGATCCCGGCGTTAGGCACCACGCACTCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 >  2:40208/1‑94 (MQ=255)
                                                                                                   ggCTGGCGATCCCGGCGTTAGGCACCACGCACTCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 <  1:40208/94‑1 (MQ=255)
                                                                                                   ggCTGGCAATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTggg                                    >  1:392283/1‑139 (MQ=255)
                                                                                                           aTCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCg                            <  2:1461711/139‑1 (MQ=255)
                                                                                                           aTCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCg                            <  1:287756/139‑1 (MQ=255)
                                                                                                           aTCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCg                            <  1:544531/139‑1 (MQ=255)
                                                                                                             cccGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAgcac                                                               >  1:1052481/1‑102 (MQ=255)
                                                                                                             cccGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAacac                                                               <  2:1052481/102‑1 (MQ=255)
                                                                                                               cGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGcg                        <  1:1605205/139‑2 (MQ=255)
                                                                                                                ggCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCg                       <  1:72997/139‑1 (MQ=255)
                                                                                                                      aGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                      >  2:1289524/1‑86 (MQ=255)
                                                                                                                      aGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                      <  1:1289524/86‑1 (MQ=255)
                                                                                                                      aGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCAt                 >  1:498287/1‑139 (MQ=255)
                                                                                                                           ccacGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGcc            <  2:723259/139‑1 (MQ=255)
                                                                                                                            cacgcacgCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                            >  2:677051/1‑74 (MQ=255)
                                                                                                                            cacgcacgCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                            <  1:677051/74‑1 (MQ=255)
                                                                                                                              cgcacgcCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCggg         >  2:979329/1‑139 (MQ=255)
                                                                                                                              cgcacgcCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 <  2:754817/67‑1 (MQ=255)
                                                                                                                              cgcacgcCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 >  1:754817/1‑67 (MQ=255)
                                                                                                                                cacgcCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGaa       <  2:392283/139‑1 (MQ=255)
                                                                                                                                 acgcCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 >  1:385032/1‑64 (MQ=255)
                                                                                                                                 acgcCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                                 <  2:385032/64‑1 (MQ=255)
                                                                                                                                     cGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATtgtg  <  1:979329/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
CCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTG  >  NC_000913/4423917‑4424188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: