Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,499,595	A→G	pseudogene (940/942 nt)	yjgX ←	pseudogene fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,595	0	A	G	100.0%	261.8 / NA	74	pseudogene (940/942 nt)	yjgX	pseudogene fragment
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/37);  total (37/37)

CTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGT  >  NC_000913/4499460‑4499724                                                                                                                                        |                                                                                                                                  cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCt                                                                                                                                >  1:1170048/1‑139 (MQ=255) cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCt                                                                                                                                <  2:631260/139‑1 (MQ=255) cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCt                                                                                                                                <  1:476187/139‑1 (MQ=255)                    tAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGgaaga                                                                                                                   >  2:583882/1‑133 (MQ=255)                    tAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGgaaga                                                                                                                   <  1:583882/133‑1 (MQ=255)                            gTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATGCGGATATTTTATACAGAATACTTTTCAGTACAGGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAgg                                                                                                     >  2:1565457/1‑139 (MQ=255)                               tGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGttt                                                                                                  >  1:1088422/1‑139 (MQ=255)                                          gggCAAATCCACCTTGTGCTGATATGAATGCGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTg                                                                                       <  1:1565457/139‑1 (MQ=255)                                          gggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATCCGGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTg                                                                                       <  1:921107/139‑1 (MQ=255)                                           ggCAAATCCACCTTGTGCTGATATGAATACGGATATCTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGTCAGATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc                                                                                      >  2:921107/1‑139 (MQ=255)                                                         gtgCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCctct                                                                        <  2:1170048/139‑1 (MQ=255)                                                          tgCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc                                                                                      >  1:1573498/1‑124 (MQ=255)                                                          tgCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc                                                                                      <  2:1573498/124‑1 (MQ=255)                                                          tgCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCctctc                                                                       >  1:1297229/1‑139 (MQ=255)                                                          tgCTGATATGAATACGGAAATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACt                                                                                            <  2:721739/118‑1 (MQ=255)                                                          tgCTGATATGAATACGGAAATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACt                                                                                            >  1:721739/1‑118 (MQ=255)                                                                 atGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTa                                                                <  2:1088422/139‑1 (MQ=255)                                                                         ggATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGTATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCAt                                                        >  2:1666036/1‑139 (MQ=255)                                                                         ggATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc                                                                                      <  1:1510155/109‑1 (MQ=255)                                                                         ggATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc                                                                                      >  2:1510155/1‑109 (MQ=255)                                                                              ttttATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAg                                                   >  2:394058/1‑139 (MQ=255)                                                                                   taCAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTg                                                                                                        <  1:508845/81‑1 (MQ=255)                                                                                   taCAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTg                                                                                                        >  2:508845/1‑81 (MQ=255)                                                                                       gAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCg                                                                                    <  2:473264/97‑1 (MQ=255)                                                                                       gAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCg                                                                                    >  1:473264/1‑97 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAgg                                                                                                                       >  2:521192/1‑61 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAgg                                                                                                                       <  1:521192/61‑1 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCt                                                                                                         <  1:63098/75‑1 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCt                                                                                                         >  2:63098/1‑75 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTg                                                                                                        >  2:129133/1‑76 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTg                                                                                                        <  1:129133/76‑1 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTg                                                                                                        >  1:522414/1‑76 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTg                                                                                                        <  2:522414/76‑1 (MQ=255)                                                                                        aaTACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATc                                         >  2:505956/1‑139 (MQ=255)                                                                                          tACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCt                                                                                                                                >  1:579128/1‑50 (MQ=255)                                                                                          tACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCt                                                                                                                                <  2:579128/50‑1 (MQ=255)                                                                                                  aGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                                                                                              >  1:59406/1‑76 (MQ=255)                                                                                                  aGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                                                                                              <  2:59406/76‑1 (MQ=255)                                                                                                  aGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCg                                                                                    >  2:332937/1‑86 (MQ=255)                                                                                                  aGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCg                                                                                    <  1:332937/86‑1 (MQ=255)                                                                                                    tACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGt                                    >  1:1128248/1‑132 (MQ=255)                                                                                                    tACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGt                                    <  2:1128248/132‑1 (MQ=255)                                                                                                    tACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAg                             <  1:394058/139‑1 (MQ=255)                                                                                                    tACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAg                             >  2:1377344/1‑139 (MQ=255)                                                                                                             cTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGaa                    <  1:1377344/139‑1 (MQ=255)                                                                                                             cTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGaa                    <  1:505956/139‑1 (MQ=255)                                                                                                                tATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc                                                                                      >  1:1498017/1‑70 (MQ=255)                                                                                                                tATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc                                                                                      <  2:1498017/70‑1 (MQ=255)                                                                                                                        tCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCa                              <  1:1660848/118‑1 (MQ=255)                                                                                                                        tCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCa                              >  2:1660848/1‑118 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCctct                                                                        <  2:1345384/74‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCctct                                                                        >  1:1345384/1‑74 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCctct                                                                        <  2:706662/74‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCctct                                                                        >  1:706662/1‑74 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa                                                      >  2:1505351/1‑92 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa                                                      <  2:1475818/92‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa                                                      <  1:1505351/92‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATaa                                                      >  1:1475818/1‑92 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACa                                                    <  1:1174245/94‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACa                                                    >  2:1174245/1‑94 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgt                                                 <  1:1228782/97‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgt                                                 >  2:1228782/1‑97 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGc                <  1:120248/130‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGc                >  2:120248/1‑130 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGt             <  1:1385898/133‑1 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGt             >  2:1385898/1‑133 (MQ=255)                                                                                                                          tGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATAttt       >  1:150770/1‑139 (MQ=255)                                                                                                                            ccATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCg                           >  1:1176956/1‑117 (MQ=255)                                                                                                                            ccATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCg                           <  2:1176956/117‑1 (MQ=255)                                                                                                                            ccATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATgg                 >  2:614413/1‑127 (MQ=255)                                                                                                                            ccATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATgg                 <  1:614413/127‑1 (MQ=255)                                                                                                                            ccATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTc     <  1:816761/139‑1 (MQ=255)                                                                                                                            ccATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTc     >  2:816761/1‑139 (MQ=255)                                                                                                                               tatatGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGt  <  1:1666036/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  CTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGT  >  NC_000913/4499460‑4499724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑