Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,515,284	C→T	G465D (GGC→GAC)	fecA ←	TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,515,284	0	C	T	100.0%	185.1 / NA	57	G465D (GGC→GAC)	fecA	TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

ACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTG  >  NC_000913/4515162‑4515406                                                                                                                           |                                                                                                                           aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc                                                                                                            >  2:491563/1‑139 (MQ=255) aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc                                                                                                            >  2:301014/1‑139 (MQ=255)       tatGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCgctgct                                                                                                      >  2:840980/1‑139 (MQ=255)           ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtg                                                                                                  >  1:1635126/1‑139 (MQ=255)           ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtg                                                                                                  >  2:824321/1‑139 (MQ=255)            tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 <  1:840980/139‑1 (MQ=255)            tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 <  2:429823/139‑1 (MQ=255)                         ccGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACg                                                                                    <  1:301014/139‑1 (MQ=255)                             cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCAtt                                                                                >  2:1195074/1‑139 (MQ=255)                                                aTGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGAttcattca                                                             >  2:1590301/1‑139 (MQ=255)                                                         ttGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGAt                                                    <  1:1273172/139‑1 (MQ=255)                                                          tGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgtagt                                                                                        >  2:1110926/1‑102 (MQ=255)                                                          tGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgtagt                                                                                        <  1:1110926/102‑1 (MQ=255)                                                             cATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                <  1:1195074/139‑1 (MQ=255)                                                             cATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                <  2:1635126/139‑1 (MQ=255)                                                              aTCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 <  2:1288387/89‑1 (MQ=255)                                                              aTCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 >  1:685753/1‑89 (MQ=255)                                                              aTCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 >  1:1288387/1‑89 (MQ=255)                                                              aTCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 <  2:685753/89‑1 (MQ=255)                                                                    aTACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                <  1:138702/84‑1 (MQ=255)                                                                    aTACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                >  2:138702/1‑84 (MQ=255)                                                                          ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                <  1:1634155/126‑1 (MQ=255)                                                                          ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                >  2:1634155/1‑126 (MQ=255)                                                                           gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGc                                                                                                                    >  1:497633/1‑57 (MQ=255)                                                                           gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGc                                                                                                                    <  2:497633/57‑1 (MQ=255)                                                                            cGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTg                                 <  1:491563/139‑1 (MQ=255)                                                                              tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACt                                                                                                                >  1:510628/1‑58 (MQ=255)                                                                              tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACt                                                                                                                <  2:510628/58‑1 (MQ=255)                                                                              tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGAt                                                    >  1:653842/1‑118 (MQ=255)                                                                              tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGAt                                                    <  2:653842/118‑1 (MQ=255)                                                                                cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 >  1:1196001/1‑71 (MQ=255)                                                                                cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                 <  2:1196001/71‑1 (MQ=255)                                                                                cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                >  2:1006985/1‑72 (MQ=255)                                                                                cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                <  1:1006985/72‑1 (MQ=255)                                                                                        tGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGcc                     <  2:777663/139‑1 (MQ=255)                                                                                        tGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGcc                     >  1:777663/1‑139 (MQ=255)                                                                                         gCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                    <  2:786746/139‑1 (MQ=255)                                                                                                  cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggt                                                                                                   >  2:242138/1‑51 (MQ=255)                                                                                                  cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggt                                                                                                   <  1:242138/51‑1 (MQ=255)                                                                                                  cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGgtg                                                                                            >  1:904357/1‑58 (MQ=255)                                                                                                  cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGgtg                                                                                            <  2:904357/58‑1 (MQ=255)                                                                                                  cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAg           >  2:1333445/1‑139 (MQ=255)                                                                                                  cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAg           >  1:1210931/1‑139 (MQ=255)                                                                                                    tATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGAt         <  1:1333445/139‑1 (MQ=255)                                                                                                          ggTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc                      <  1:284817/120‑1 (MQ=255)                                                                                                          ggTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc                      >  2:284817/1‑120 (MQ=255)                                                                                                           gTCGTAAGGACTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTg  >  2:129764/1‑139 (MQ=255)                                                                                                            tCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                              >  1:668206/1‑46 (MQ=255)                                                                                                            tCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                              <  2:668206/46‑1 (MQ=255)                                                                                                             cGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtgg                                                                                                    <  1:955175/39‑1 (MQ=255)                                                                                                             cGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtgg                                                                                                    >  2:955175/1‑39 (MQ=255)                                                                                                                      ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc                                                                              >  2:327352/1‑52 (MQ=255)                                                                                                                      ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc                                                                              <  1:327352/52‑1 (MQ=255)                                                                                                                      ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCgg                            >  2:101482/1‑102 (MQ=255)                                                                                                                      ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCgg                            <  1:101482/102‑1 (MQ=255)                                                                                                                      ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                    <  2:683199/110‑1 (MQ=255)                                                                                                                      ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                    >  1:683199/1‑110 (MQ=255)                                                                                                                           |                                                                                                                           ACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTG  >  NC_000913/4515162‑4515406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑