| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,553,337 | C→T | P146L (CCG→CTG) | uxuB → | D‑mannonate oxidoreductase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,553,337 | 0 | C | T | 97.6% | 126.7 / ‑6.3 | 42 | P146L (CCG→CTG) | uxuB | D‑mannonate oxidoreductase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (19/22); minor base A (0/1); total (19/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATA > NC_000913/4553205‑4553459 | aTCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGAt < 2:1482763/139‑1 (MQ=255) ggAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAg < 1:1560262/139‑1 (MQ=255) gAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGc < 1:1621144/139‑1 (MQ=255) cgcgATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGa < 1:68837/128‑1 (MQ=255) cgcgATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGa > 2:68837/1‑128 (MQ=255) gcgATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGt < 2:393056/139‑1 (MQ=255) cgATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTc < 1:795854/139‑1 (MQ=255) cGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCAc < 1:629702/98‑1 (MQ=255) cGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCAc > 2:629702/1‑98 (MQ=255) cAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCAAAGTCCGCGATTGGTTACa < 1:1241029/139‑1 (MQ=255) cGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCg < 1:1470867/113‑1 (MQ=255) cGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCg < 2:7050/113‑1 (MQ=255) cGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCg > 2:1470867/1‑113 (MQ=255) cGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCg > 1:7050/1‑113 (MQ=255) aCGGACACGGAAGAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCAGCTGATCAAGTACGTTCTGGTAAGCCCGGCTGCGCCGAAGTCCGCGATTGGTTACATCGTCGTAGCGTtgcgtctgc < 1:1375562/139‑1 (MQ=255) gTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGaa < 2:848030/98‑1 (MQ=255) gTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGaa > 1:848030/1‑98 (MQ=255) tCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCcgcg > 1:486790/1‑104 (MQ=255) tCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCcgcg < 2:486790/104‑1 (MQ=255) aCGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGa > 1:1413900/1‑85 (MQ=255) aCGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGa < 2:1413900/85‑1 (MQ=255) aCGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTg > 1:422484/1‑139 (MQ=255) aGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGc > 2:549302/1‑59 (MQ=255) aGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGc < 1:549302/59‑1 (MQ=255) cTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGa < 2:265652/89‑1 (MQ=255) cTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGa > 1:265652/1‑89 (MQ=255) cTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGtt > 2:227979/1‑139 (MQ=255) aTGCGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTAc > 2:419519/1‑139 (MQ=255) cGGCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGt > 2:1355934/1‑139 (MQ=255) gCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 1:313727/1‑139 (MQ=255) gCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa < 2:313727/139‑1 (MQ=255) gCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 1:24421/1‑139 (MQ=255) gCAAGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGa > 2:73189/1‑139 (MQ=255) aaGCGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAATCCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATg < 1:1392053/139‑1 (MQ=255) cgctCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACg < 2:1085377/127‑1 (MQ=255) cGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCg > 2:1079061/1‑91 (MQ=255) cGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCg < 1:1079061/91‑1 (MQ=255) cGGTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACg > 1:1085377/1‑130 (MQ=255) ggTCAACTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCt < 1:419519/139‑1 (MQ=255) cTGGATCTCAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATa < 2:422484/139‑1 (MQ=255) tctcAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTtgcgtc < 1:342164/86‑1 (MQ=255) tctcAATAACCTGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTtgcgtc > 2:342164/1‑86 (MQ=255) | ATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCCGCGATTGGTTACATCGTCGAAGCGTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATA > NC_000913/4553205‑4553459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |