Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,462	C→T	A350V (GCG→GTG)	prfC →	peptide chain release factor RF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,462	0	C	T	100.0%	182.0 / NA	56	A350V (GCG→GTG)	prfC	peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/32);  total (24/32)

CAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTT  >  NC_000913/4610328‑4610591                                                                                                                                       |                                                                                                                                  cAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGt                                                                                                                               <  2:994683/139‑1 (MQ=255)          cccGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCg                                                                                                                      <  1:663740/139‑1 (MQ=255)              aaaCACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCg                                                                                                                      <  1:1160613/135‑1 (MQ=255)              aaaCACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCg                                                                                                                      >  2:1160613/1‑135 (MQ=255)              aaaCACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg                                                                                                                  >  2:958062/1‑139 (MQ=255)                     gcgaccgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCAc                                                                                                                   <  1:642514/131‑1 (MQ=255)                     gcgaccgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCAc                                                                                                                   >  2:642514/1‑131 (MQ=255)                       gaccgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGaagaag                                                                                                         <  1:958062/139‑1 (MQ=255)                              tGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATcc                                                                                                  <  2:1524026/139‑1 (MQ=255)                                    ttATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTACCCGGGCGa                                                                                            <  1:349707/139‑1 (MQ=255)                                          gtgtGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCt                                                                                      <  1:568851/139‑1 (MQ=255)                                           tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATc                                                                                                   <  2:1304710/125‑1 (MQ=255)                                           tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATc                                                                                                   >  1:1304710/1‑125 (MQ=255)                                            gtggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCg                                                                                    <  1:576994/139‑1 (MQ=255)                                             tggtgTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCgg                                                                                   <  1:67382/139‑1 (MQ=255)                                                           tatGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACg                                                                     <  1:943622/139‑1 (MQ=255)                                                             tGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTga                                                                                                              >  1:1555931/1‑96 (MQ=255)                                                             tGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTga                                                                                                              <  2:1555931/96‑1 (MQ=255)                                                               aaaaaGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCAc                                                                 >  1:393833/1‑139 (MQ=255)                                                                     gCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGa                                                                                            >  2:903247/1‑106 (MQ=255)                                                                     gCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGa                                                                                            <  1:903247/106‑1 (MQ=255)                                                                     gCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCa                                                           >  1:450383/1‑139 (MQ=255)                                                                     gCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCa                                                           >  2:903609/1‑139 (MQ=255)                                                                              tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  <  2:450383/139‑1 (MQ=255)                                                                                cgcCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                  >  2:883773/1‑121 (MQ=255)                                                                                cgcCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                  <  1:883773/121‑1 (MQ=255)                                                                                     ggTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTca                                                                                                                      >  1:1088757/1‑63 (MQ=255)                                                                                     ggTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCg                                                                                                                      <  2:1088757/64‑1 (MQ=255)                                                                                       tGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCTGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  >  1:987126/1‑130 (MQ=255)                                                                                       tGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  <  2:987126/130‑1 (MQ=255)                                                                                        gcgcACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTAcc                                        <  2:183744/139‑1 (MQ=255)                                                                                          gcACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                  >  2:229068/1‑111 (MQ=255)                                                                                          gcACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                  <  1:229068/111‑1 (MQ=255)                                                                                                cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGt                                                                                                                               <  1:1482434/44‑1 (MQ=255)                                                                                                cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGt                                                                                                                               >  2:1482434/1‑44 (MQ=255)                                                                                                cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa                                                                       >  2:1364010/1‑100 (MQ=255)                                                                                                cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa                                                                       <  1:1364010/100‑1 (MQ=255)                                                                                                       tgtgGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGatgatg                         <  1:903609/139‑1 (MQ=255)                                                                                                        gtggtgATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                  <  1:667544/97‑1 (MQ=255)                                                                                                        gtggtgATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCa                                                                  >  2:667544/1‑97 (MQ=255)                                                                                                        gtggtgATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  <  2:1409841/113‑1 (MQ=255)                                                                                                        gtggtgATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  >  1:1409841/1‑113 (MQ=255)                                                                                                                     aCGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  >  1:715439/1‑100 (MQ=255)                                                                                                                     aCGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  <  2:715439/100‑1 (MQ=255)                                                                                                                        cgcTGACCTTTATGGTGGGTGACCGTTCGCACGTTgaag                                                                                                            <  2:1287195/39‑1 (MQ=38)                                                                                                                        cgcTGACCTTTATGGTGGGTGACCGTTCGCACGTTgaag                                                                                                            >  1:1287195/1‑39 (MQ=38)                                                                                                                          cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGa                                                         <  2:1446545/88‑1 (MQ=255)                                                                                                                          cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGa                                                         >  1:1446545/1‑88 (MQ=255)                                                                                                                          cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  >  1:97163/1‑95 (MQ=255)                                                                                                                          cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa                                                  <  2:97163/95‑1 (MQ=255)                                                                                                                           tGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGa                                                                                            >  1:749176/1‑52 (MQ=255)                                                                                                                           tGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGa                                                                                            <  2:749176/52‑1 (MQ=255)                                                                                                                             aCCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACt   >  2:442727/1‑139 (MQ=255)                                                                                                                              ccTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACtt  <  1:442727/139‑1 (MQ=255)                                                                                                                                  tATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGc                                                                                  <  2:1450933/55‑1 (MQ=255)                                                                                                                                  tATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGc                                                                                  >  1:1450933/1‑55 (MQ=255)                                                                                                                                       |                                                                                                                                  CAACATGGACCCGAAACACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACTT  >  NC_000913/4610328‑4610591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑