Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,208,534 | Δ1 bp | coding (403/414 nt);coding (586/603 nt) | tfaP → tfaE ← |
e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,208,534 | 0 | C | . | 98.2% | 204.4 / ‑3.1 | 55 | coding (403/414 nt) coding (586/603 nt) | tfaP tfaE | e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (29/25); total (29/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAA > NC_000913/1208400‑1208662 | gTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGcc < 1:1055136/139‑1 (MQ=255) gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTTGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg < 1:51790/139‑1 (MQ=255) gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg < 1:805356/139‑1 (MQ=255) aGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGcgcg < 1:711766/139‑1 (MQ=255) aagaagATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTgg > 1:86603/1‑139 (MQ=255) gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGAt < 2:1218347/114‑1 (MQ=255) gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGAt > 1:1218347/1‑114 (MQ=255) gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGcgcg < 2:67278/127‑1 (MQ=255) gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGcgcg > 1:67278/1‑127 (MQ=255) gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCt > 2:854522/1‑139 (MQ=255) gCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGc > 2:1157246/1‑139 (MQ=255) gAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGcc < 2:955031/102‑1 (MQ=255) gAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGcc > 1:955031/1‑102 (MQ=255) aaCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCa < 2:487/139‑1 (MQ=255) aTTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt > 1:1153815/1‑136 (MQ=255) aTTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt < 2:1153815/136‑1 (MQ=255) ttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg < 2:86603/139‑1 (MQ=255) ttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg < 2:551310/139‑1 (MQ=255) ttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCACATATTGAATGGCTTACGCCTCCGGCAGTTC‑GGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg < 1:801991/139‑1 (MQ=255) cTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTg < 2:55410/103‑1 (MQ=255) cTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTg > 1:55410/1‑103 (MQ=255) cTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGcac > 1:1115122/1‑135 (MQ=255) cTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGcac < 2:1115122/135‑1 (MQ=255) aGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAg > 1:611301/1‑139 (MQ=255) cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg < 2:854440/80‑1 (MQ=17) cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg > 1:854440/1‑80 (MQ=17) cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg > 1:172758/1‑115 (MQ=255) cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg < 2:172758/115‑1 (MQ=255) cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTg > 1:177288/1‑139 (MQ=255) cATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCg < 1:616082/139‑1 (MQ=255) aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt < 2:611301/139‑1 (MQ=255) ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg < 1:1206016/96‑1 (MQ=255) ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg > 2:1206016/1‑96 (MQ=255) ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGcac < 2:109253/107‑1 (MQ=255) ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGcac > 1:109253/1‑107 (MQ=255) tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt > 2:432846/1‑139 (MQ=255) tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt > 1:214780/1‑139 (MQ=255) tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt > 1:771274/1‑139 (MQ=255) tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt > 1:391096/1‑139 (MQ=255) tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt > 2:953166/1‑139 (MQ=255) ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGt < 1:9750/139‑1 (MQ=255) cAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTAt > 2:1191925/1‑64 (MQ=11) cAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTAt < 1:1191925/64‑1 (MQ=11) cAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTc > 1:134036/1‑139 (MQ=255) cAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTc > 1:203784/1‑139 (MQ=255) tGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt > 2:1172655/1‑75 (MQ=11) tGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt < 1:1172655/75‑1 (MQ=11) gcttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAAttt > 1:303560/4‑139 (MQ=255) cttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg < 2:750049/36‑1 (MQ=14) cttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg > 1:750049/3‑38 (MQ=14) cttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTc > 2:923083/3‑139 (MQ=255) cttACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTc > 1:339106/3‑139 (MQ=255) tACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAg < 2:134036/139‑1 (MQ=255) gCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATCTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTg > 2:759421/1‑139 (MQ=255) cTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGaa < 1:1035135/139‑1 (MQ=255) | GTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAA > NC_000913/1208400‑1208662 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |