Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,655,360 (C)5→4 coding (314/327 nt) ynfA ← UPF0060 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,655,3560C.100.0% 164.8 / NA 40coding (318/327 nt)ynfAUPF0060 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (21/19);  total (21/19)

GAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAG  >  NC_000913/1655218‑1655489
                                                                                                                                          |                                                                                                                                     
gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTgcgc                                                                                                                                       >  1:1137091/1‑139 (MQ=255)
  aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                     <  1:138499/139‑1 (MQ=255)
  aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                     <  1:734522/139‑1 (MQ=255)
    aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                                   <  2:1137091/139‑2 (MQ=255)
    aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGcccca                                                                                                                                   <  2:429676/139‑2 (MQ=255)
       gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                                <  1:233652/139‑5 (MQ=255)
            aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                          <  2:1082307/139‑1 (MQ=255)
            aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                          <  1:302608/139‑1 (MQ=255)
              tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                        <  2:1078217/139‑1 (MQ=255)
                aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAAt                                                                                                                      <  1:988341/139‑1 (MQ=255)
                     aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCa                                                                                                                 >  1:1148214/1‑139 (MQ=255)
                                        cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                              >  2:126575/1‑139 (MQ=255)
                                            gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                          >  2:861670/1‑139 (MQ=255)
                                             tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                         >  1:750645/1‑139 (MQ=255)
                                                   gTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                                   <  2:1080196/139‑1 (MQ=255)
                                                       aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                               >  2:430484/1‑139 (MQ=255)
                                                       aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                               <  2:750645/139‑1 (MQ=255)
                                                                   aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                   <  2:1148214/139‑1 (MQ=255)
                                                                       aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATc                                                                                             >  2:1061106/1‑109 (MQ=255)
                                                                       aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATc                                                                                             <  1:1061106/109‑1 (MQ=255)
                                                                        aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                                <  1:904238/74‑5 (MQ=255)
                                                                        aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                                >  2:904238/1‑70 (MQ=255)
                                                                              tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  1:594764/114‑1 (MQ=255)
                                                                              tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  2:238012/114‑1 (MQ=255)
                                                                              tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 >  2:594764/1‑114 (MQ=255)
                                                                              tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 >  1:238012/1‑114 (MQ=255)
                                                                               ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                       >  2:537082/1‑139 (MQ=255)
                                                                                 gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCa                                                                                                                 >  1:1078604/1‑79 (MQ=255)
                                                                                 gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCa                                                                                                                 <  2:1078604/79‑1 (MQ=255)
                                                                                   cAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCACAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                          <  2:196625/132‑1 (MQ=255)
                                                                                   cAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCACAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                          >  1:196625/1‑132 (MQ=255)
                                                                                      cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                >  1:17323/1‑139 (MQ=255)
                                                                                         acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACCCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                             <  1:861670/139‑1 (MQ=255)
                                                                                           acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                           >  2:948240/1‑139 (MQ=255)
                                                                                               ccTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  1:537082/139‑1 (MQ=255)
                                                                                                          atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                            >  1:90164/1‑139 (MQ=255)
                                                                                                          atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                            >  1:528289/1‑139 (MQ=255)
                                                                                                           ttGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                      <  1:942639/80‑1 (MQ=255)
                                                                                                           ttGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                      >  2:942639/1‑80 (MQ=255)
                                                                                                           ttGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                           >  2:1103082/1‑139 (MQ=255)
                                                                                                             gATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGt                         >  1:880504/1‑139 (MQ=255)
                                                                                                                aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                       >  1:989904/1‑74 (MQ=255)
                                                                                                                aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                       <  2:989904/74‑1 (MQ=255)
                                                                                                                  aaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCTGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCATAAcg                                             >  2:823750/1‑114 (MQ=255)
                                                                                                                  aaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                             <  1:823750/114‑1 (MQ=255)
                                                                                                                                cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                         <  2:805722/86‑1 (MQ=255)
                                                                                                                                cttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                         >  1:805722/3‑88 (MQ=255)
                                                                                                                                    cGTGCGCCCCAGACCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg  <  2:90164/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAG  >  NC_000913/1655218‑1655489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: