Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,821,867 (A)8→7 intergenic (‑248/+51) chbB ← / ← osmE N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,8600A.97.4% 304.7 / 1.9 77intergenic (‑241/+58)chbB/osmEN,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (43/32);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

ACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821747‑1821994
                                                                                                                 |                                                                                                                                      
aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   >  2:1143960/1‑134 (MQ=255)
aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   <  1:1143960/134‑1 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCgt                                                                                                          >  1:132705/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATgg                                                                                                                     <  1:409677/127‑1 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATgg                                                                                                                     >  2:409677/1‑127 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:810192/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:112611/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:1087420/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:57631/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:244138/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:512064/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:357126/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:406587/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:490566/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:263771/1‑139 (MQ=255)
     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑AAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        >  2:1197469/1‑139 (MQ=255)
          tAGTATTTCTTTGACCTTATAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACt                                                                                                   >  2:165053/1‑139 (MQ=255)
             tATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCa                                                                                                 <  2:406587/139‑1 (MQ=255)
                  cTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCtt                                                                                            >  2:255254/1‑139 (MQ=255)
                      gACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                        >  2:239873/1‑139 (MQ=255)
                        ccTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  1:244138/139‑1 (MQ=255)
                                    tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                                  <  2:764444/115‑1 (MQ=255)
                                    tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                                  >  1:764444/1‑115 (MQ=255)
                                      tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  1:941600/1‑139 (MQ=255)
                                       ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:263771/139‑1 (MQ=255)
                                         ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:255254/139‑1 (MQ=255)
                                         ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:357126/139‑1 (MQ=255)
                                          cTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:132705/139‑1 (MQ=255)
                                            tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaacc                                                                                                                                >  2:402312/1‑76 (MQ=255)
                                            tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaacc                                                                                                                                <  1:402312/78‑3 (MQ=255)
                                            tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGcc                                                                  >  2:236035/1‑139 (MQ=255)
                                              aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  2:512064/139‑1 (MQ=255)
                                                  gtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGa                                                            >  1:580496/1‑139 (MQ=255)
                                                   tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  1:112611/139‑1 (MQ=255)
                                                    ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGcc                                                                  >  2:1189313/1‑131 (MQ=255)
                                                    ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGcc                                                                  <  1:1189313/131‑1 (MQ=255)
                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             <  2:221275/84‑1 (MQ=255)
                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             >  1:221275/1‑84 (MQ=255)
                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  2:733057/1‑139 (MQ=255)
                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  1:130474/1‑139 (MQ=255)
                                                         tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:1215830/123‑1 (MQ=255)
                                                         tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     >  1:1215830/1‑123 (MQ=255)
                                                          gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:1087420/139‑1 (MQ=255)
                                                          gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  2:580496/139‑1 (MQ=255)
                                                             ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:905180/1‑139 (MQ=255)
                                                               tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               >  2:292491/1‑139 (MQ=255)
                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCttt                                                                                           <  1:216353/91‑1 (MQ=255)
                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCttt                                                                                           >  2:216353/1‑91 (MQ=255)
                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:280817/113‑1 (MQ=255)
                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     >  2:280817/1‑113 (MQ=255)
                                                                    acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:341313/139‑1 (MQ=255)
                                                                           gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                   >  1:8790/1‑139 (MQ=255)
                                                                                   aatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCg                           <  2:57631/139‑1 (MQ=255)
                                                                                     ttCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTTTGATAACCGGa                         <  2:905180/139‑1 (MQ=255)
                                                                                      tCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAg                        <  1:503694/139‑1 (MQ=255)
                                                                                       cgcgAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGt                       >  2:81998/1‑139 (MQ=255)
                                                                                             gATAATTAAGAACTCAGGCC‑‑AAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACTAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:1197469/139‑1 (MQ=255)
                                                                                              aTAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:490566/139‑1 (MQ=255)
                                                                                              aTAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:292491/139‑1 (MQ=255)
                                                                                              aTAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:239873/139‑1 (MQ=255)
                                                                                                   tAAGAACTCAGGCCAAAAAAACCTGCGCAATGGCCGGTTTCCGTTGTTACTCAATCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  1:802637/139‑1 (MQ=255)
                                                                                                   tAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:941600/139‑1 (MQ=255)
                                                                                                       aaCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGt       >  2:1186354/1‑139 (MQ=255)
                                                                                                        aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                             <  2:839946/68‑1 (MQ=255)
                                                                                                        aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                             >  1:839946/1‑68 (MQ=255)
                                                                                                        aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  1:1152541/1‑139 (MQ=255)
                                                                                                         cTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:1030200/92‑1 (MQ=255)
                                                                                                         cTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  2:1030200/1‑92 (MQ=255)
                                                                                                         cTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTat     <  2:130474/139‑1 (MQ=255)
                                                                                                          tCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatc    >  2:1164351/1‑139 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      >  2:503715/1‑55 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  1:503715/55‑1 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAg                        <  2:429080/117‑1 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAg                        >  1:429080/1‑117 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:633959/1‑139 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  1:514608/1‑139 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  1:521372/1‑139 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:126622/1‑139 (MQ=255)
                                                                                                            aGGCCAAAAAAACCGGCGAAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:802637/1‑139 (MQ=255)
                                                                                                                 |                                                                                                                                      
ACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821747‑1821994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: