Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,765,829 (C)5→4 intergenic (‑53/‑89) yfjM ← / → rnlA CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,765,8250C.100.0% 276.8 / NA 66intergenic (‑49/‑93)yfjM/rnlACP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (33/33);  total (33/33)

CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGC  >  NC_000913/2765687‑2765958
                                                                                                                                             |                                                                                                                                     
cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGc                                                                                                                                       <  1:1133442/139‑1 (MQ=255)
   gTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                                                                     >  1:352992/1‑138 (MQ=255)
   gTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                                                                     <  2:352992/138‑1 (MQ=255)
        ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                                                                      >  1:541771/1‑132 (MQ=255)
        ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                                                                      <  2:541771/132‑1 (MQ=255)
                   ttACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTaaa                                                                                                                   <  2:571643/139‑1 (MQ=255)
                           tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                              >  2:714388/1‑136 (MQ=255)
                           tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                              <  1:714388/136‑1 (MQ=255)
                           tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCaa                                                                                                           >  1:1218381/1‑139 (MQ=255)
                           tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCaa                                                                                                           >  2:1002756/1‑139 (MQ=255)
                             tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                         <  1:583957/139‑1 (MQ=255)
                              cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATc                                                                                                        >  2:150506/1‑139 (MQ=255)
                               gAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCa                                                                                                       <  2:531120/139‑1 (MQ=255)
                                aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCTCGGTGGACGATCCTGCCCTATAGTTGCAGTGCCCCTATTAAGGTGGTTAAATTTAGCAAACCa                                                                                                       >  1:755958/1‑139 (MQ=255)
                                aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCaa                                                                                                      >  2:1021727/1‑139 (MQ=255)
                                aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAAt                                                                                                     >  2:130056/1‑139 (MQ=255)
                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                    <  1:150506/139‑1 (MQ=255)
                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                         >  2:1053685/1‑131 (MQ=255)
                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                         <  1:1053685/131‑1 (MQ=255)
                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                 >  2:617276/1‑139 (MQ=255)
                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTCGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                 >  1:501037/1‑139 (MQ=255)
                                        ctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCa                                                                                                                             >  1:893090/1‑108 (MQ=255)
                                        ctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCa                                                                                                                             <  2:893090/108‑1 (MQ=255)
                                           gggtgtgttcggAAGGGGTTTACCGAAAGAAGGACCTCAACCCCCATTTTGTGGAACGCCCCCCCCGGTATACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGTCTAAATTTAGCAAATCAATACACTTCAg                                                                                            <  2:755958/129‑1 (MQ=255)
                                               agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                       <  1:617276/139‑1 (MQ=255)
                                                gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                     <  1:130056/139‑1 (MQ=255)
                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                     <  2:501037/139‑1 (MQ=255)
                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                     <  1:275396/139‑1 (MQ=255)
                                                        agagGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGt                                                                              <  1:1175588/139‑1 (MQ=255)
                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                    <  2:1198988/114‑1 (MQ=255)
                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                    >  1:1198988/1‑114 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                            <  1:106791/103‑1 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                            >  2:106791/1‑103 (MQ=255)
                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                         <  2:169742/136‑1 (MQ=255)
                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                         >  1:169742/1‑136 (MQ=255)
                                                                  cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                     <  2:1218381/139‑1 (MQ=255)
                                                                       cGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                                                                >  2:151275/1‑139 (MQ=255)
                                                                               atcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                                                        <  2:301984/139‑1 (MQ=255)
                                                                                tcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt                                                       <  1:1002756/139‑1 (MQ=255)
                                                                                        cATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTATCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCa                                               >  2:734208/1‑139 (MQ=255)
                                                                                                     tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                   >  1:86344/1‑139 (MQ=255)
                                                                                                      gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                     >  1:1154078/1‑88 (MQ=255)
                                                                                                      gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                     <  2:1154078/88‑1 (MQ=255)
                                                                                                       cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                            <  2:139128/64‑1 (MQ=255)
                                                                                                       cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                            >  1:139128/1‑64 (MQ=255)
                                                                                                       cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                 >  1:293692/1‑139 (MQ=255)
                                                                                                           cccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAg                             <  1:362743/139‑1 (MQ=255)
                                                                                                            ccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                      <  1:548228/113‑1 (MQ=255)
                                                                                                            ccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                      >  2:548228/1‑113 (MQ=255)
                                                                                                                  aGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                       <  2:86344/139‑1 (MQ=255)
                                                                                                                  aGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                       <  2:293692/139‑1 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                              >  1:574803/1‑43 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                              <  2:680732/43‑1 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                              <  2:574803/43‑1 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                              >  1:680732/1‑43 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTg                                                                               >  1:231452/1‑74 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTg                                                                               <  2:231452/74‑1 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAg                             <  2:1094543/124‑1 (MQ=255)
                                                                                                                           tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAg                             >  1:1094543/1‑124 (MQ=255)
                                                                                                                             cccTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                            >  2:94887/1‑43 (MQ=255)
                                                                                                                             cccTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                            <  1:94887/43‑1 (MQ=255)
                                                                                                                                  taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                    >  2:720658/1‑46 (MQ=255)
                                                                                                                                  taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                    <  1:720658/46‑1 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                         >  2:989572/1‑36 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                                                         <  1:989572/36‑1 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                       <  2:752760/54‑1 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                       >  1:752760/1‑54 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc  >  2:692234/1‑139 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc  >  2:109929/1‑139 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc  >  1:810480/1‑139 (MQ=255)
                                                                                                                                       ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc  >  1:43819/1‑139 (MQ=255)
                                                                                                                                             |                                                                                                                                     
CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGC  >  NC_000913/2765687‑2765958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: